Skip to main content
SpringerLink
Log in

Genetic variability of Colletotrichum lindemuthianum isolates from Slovenia and resistance of local Phaseolus vulgaris germplasm

Genetische Variabilität slowenischer Isolate von Colletotrichum lindemuthianum und Resistenz lokalen Keimplasmas von Phaseolus vulgaris

  • Published:
Journal of Plant Diseases and Protection Aims and scope Submit manuscript

Abstract

Bean anthracnose caused by Colletotrichum lindemuthianum is one of the most destructive diseases in all bean growing areas. AFLP analysis was used to assess genetic variability and relationships among C. lindemuthianum isolates from Slovenia. Isolates were obtained from different common bean growing regions in Slovenia and have previously been characterized as races 23, 55, 103 and 131. AFLP clustering revealed two main groups that did not clearly correspond to race classification based on pathogenicity towards differential common bean cultivars. Local common bean germplasm as well as the set of 12 international differential bean cultivars were evaluated for resistance against the four C. lindemuth-ianum races that occur in Slovenia. None of the 26 local common bean landraces and cultivars was resistant to all four races; however, six genotypes reacted as moderately resistant. Considerably higher level of resistance was recorded among differential common bean cultivars, particularly those derived from the Mesoamerican germplasm. Introgression of some major anthracnose resistance genes from the Mesoamerican germplasm is therefore recommended to develop resistant common bean cultivars for our production system.

Zusammenfassung

Die durch Colletotrichum lindemuthianum verursachte Brenn-fleckenkrankheit (Anthraknose) ist eine der bedeutendsten Erkrankungen der Gartenbohne (Phaseolus vulgaris) in allen Anbaugebieten. Die genetische Variabilität und die Verwandt-schaft slowenischer Isolate von C. lindemuthianum wurde mit-tels AFLP untersucht. Die Isolate stammten aus verschiedenen slowenischen Anbaugebieten der Gartenbohne und wurden bereits früher als den Rassen 23, 55, 103 und 131 zugehörig charakterisiert. Eine AFLP-Clusteranalyse ergab zwei Haupt-gruppen, die nicht eindeutig mit der auf der Pathogenität ge-genüber Differentialsorten basierenden Rassenklassifikation übereinstimmte. Sowohl lokales Keimplasma als auch eine Gruppe von 12 internationalen Differentialsorten der Garten-bohne wurde hinsichtlich ihrer Resistenz gegenüber den vier nachgewiesenen slowenischen Rassen von C. lindemuthianum untersucht. Keine der 26 lokalen Landrassen war gegenüber allen vier Rassen des Erregers resistent, allerdings zeigten sechs Genotypen eine moderate Resistenzreaktion. Die Diffe-rentialsorten der Gartenbohne, insbesondere jene aus meso-amerikanischem Keimplasma gezüchteten, besa ß en beträcht-lich höhere Resistenzgrade als die lokalen Landrassen. Eine Inrogression bedeutender Anthraknose-Resistenzgene aus mesoamerikanischem Keimplasma kann daher für die Züchtung resistenter Gartenbohnensorten in unserem Produk-tionssystem empfohlen werden.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Alam, M., K. Rudolph, 1988: Recent occurrence and characterization of the races of bean anthracnose (Colletotrichum lindemuthianum) in West Germany. J. Plant Dis. Protect. 95, 406–413.

    Google Scholar 

  • Alam, M., K. Rudolph, 1993: Occurrence and characterization of the races of bean anthracnose (Colletotrichum lindemuth-ianum) in Turkey. Phytopathol. Medit. 32, 228–234.

    Google Scholar 

  • Alzate-Marin, A.L., G.S. Baia, T.J. Paula Jr., G.A. Carvalho, E.G. Barros, M.A. Moreira, 1997: Inheritance of anthracnose resistance in common bean differential cultivar AB 136. Plant Dis. 81, 996–998.

    Article  Google Scholar 

  • Alzate-Marin, A.L., M.R. Costa, K.M. Arruda, E.G. Barros, M.A. Moreira, 2003: Characterization of the anthracnose resistance gene present in Ouro Negro (Honduras 35) common bean cultivar. Euphytica 133, 165–169.

    Article  CAS  Google Scholar 

  • Alzate-Marin, A.L., K.A. Souza, M.G. Silva, E.J. Oliveira, M.A. Moreira, E.G. Barros, 2007: Genetic characterization of anthracnose resistance genes Co-43 and Co-9 in common bean cultivar tlalnepantla 64 (PI 207262). Euphytica 154, 1–8.

    Article  CAS  Google Scholar 

  • Ansari, K.I., N. Palacios, C. Araya, T. Langin, D. Egan, F.M. Doohan, 2004: Pathogenic and genetic variability among Colletotrichum lindemuthianum isolates of different geographic origins. Plant Pathol. 53, 635–642.

    Article  CAS  Google Scholar 

  • Balardin, R.S., A.M. Jarosz, J.D. Kelly, 1997: Virulence and molecular diversity in Colletotrichum lindemuthianum from South, Central, and North America. Phytopathology 87, 1187–1191.

    Article  Google Scholar 

  • Fernández, M.T., M. Fernández, A. Casares, R. Rodriguez, M. Fueyo, 2000: Bean germplasm evaluation for anthracnose resistance and characterization of agronomic traits: A new physiological strain of Colletotrichum lindemuthianum infecting Phaseolus vulgaris L. in Spain. Euphytica 114, 143–149.

    Article  Google Scholar 

  • Fouilloux, G., 1976: Bean anthracnose: new genes of resistance. Ann. Rep. Bean Improvement Coop. 19, 36–37.

    Google Scholar 

  • Gonçalves-Vidigal, M.C., J.D. Kelly, 2006: Inheritance of anthracnose resistance in the common bean cultivar Widusa. Euphytica 151, 411–419.

    Article  Google Scholar 

  • Gonçalves-Vidigal, M.C., C.R. Silva, P.S. Vidigal Filho, A. GONELA, M.V. KVITSCHAL, 2007: Allelic relationships of anthra -cnose (Colletotrichum lindemuthianum) resistance in the common bean (Phaseolus vulgaris L.) cultivar Michelite and the proposal of a new anthracnose resistance gene, Co-11. Genet. Mol. Biol. 30, 589–593.

    Article  Google Scholar 

  • González, M., R. Rodríguez, M.E. Zavala, J.L. Jacobo, F. Hernández, J. Acosta, O. Martínez, J. Simpson, 1998: Characterization of Mexican isolates of Colletotrichum lindemuth-ianum by using differential cultivars and molecular markers. Phytopathology 88, 292–199.

    Article  PubMed  Google Scholar 

  • Gonzáles-Chavira, M., R. Rodríguez Guerra, F. Hernández-Godínez, J.A. Acosta-Gallegos, O. Martínez de la Vega, J. Simpson, 2004: Analysis of pathotypes of Colletotrichum lindemuthianum found in the central region of Mexico and resistance in elite germplasm of Phaseolus vulgaris. Plant Dis. 88, 152–156.

    Article  Google Scholar 

  • Hubbeling, N., 1977: The new iota race of Colletotrichum lindemuthianum. Ann. Rep. Bean Improvement Coop. 20, 58.

    Google Scholar 

  • Kelly, J.D., L. Afanador, L.S. Cameron, 1994: New races of Colletotrichum lindemuthianum in Michigan and implications in dry bean resistance breeding. Plant Dis. 78, 892–894.

  • Kelly, J.D., V.A. Vallejo, 2004: A comprehensive review of the major genes conditioning resistance to anthracnose in common bean. Hortscience 39, 1196–1207.

    CAS  Google Scholar 

  • Krüger, J., G.M. Hoffmann, N. Hubbeling, 1977: The kappa ra ce of Colletotrichum lindemuthianum and sources of resistance to anthracnose in Phaseolus beans. Euphytica 26, 23–25.

    Article  Google Scholar 

  • Kump, B., B. Javornik, 1996: Evaluation of genetic variability among common buckwheat (Fagopyrum esculentum Moench) populations by RAPD markers. Plant Sci. 114, 149–158.

    Article  CAS  Google Scholar 

  • Mahuku, G.S., C.E. Jara, C. Cajiao, S. Beebe, 2002: Sources of resistance to Colletotrichum lindemuthianum in the secondary gene pool of Phaseolus vulgaris and in crosses of primary and secondary gene pools. Plant Dis. 86, 1383–1387.

    Article  CAS  Google Scholar 

  • Mahuku, G.S., J.J. Riascos, 2004: Virulence and molecular diversity within Colletotrichum lindemuthianum isolates from Andean and Mesoamerican bean varieties and regions. Eur. J. Plant Pathol. 110, 253–263.

    Article  CAS  Google Scholar 

  • Mastenbroek, C., 1960: A breeding programme for resistance to anthracnose in dry shell haricot beans based on a new gene. Euphytica 9, 177–184.

    Article  Google Scholar 

  • Melotto, M., J.D. Kelly, 2000: An allelic series at the Co-1 locus conditioning resistance to anthracnose in common bean of Andean origin. Euphytica 116, 143–149.

    Article  Google Scholar 

  • Munda, A., M. Žerjav, J. ŠUŠtar-Vozlic, 2002: Identification of physiological races of Colletotrichum lindemuthianum occurring in Slovenia. Acta Biologica Slovenica 45, 3–7.

    Google Scholar 

  • Pastor-Corrales, M.A., 1991: Estandarización de variedades diferenciales y de designación de razas de Colletotrichum lindemuthianum. Phytopathology 81, 694.

    Google Scholar 

  • Pavlicek, A., S. Hrda, J. Flegr, 1999: FreeTree–freeware program for construction of phylogenetic trees on the basis of distance data and bootstrap/jackknife analysis of the tree robustness, Application in the RapD analysis of genus Frenkelia. Folia Biol. 45, 97–99.

    CAS  Google Scholar 

  • Radišek, S., J. Jakše, B. Javornik, 2006: Genetic variability and virulence among Verticillium albo-atrum isolates from hop. Eur. J. Plant Pathol. 116, 301–314.

    Article  Google Scholar 

  • Rodríguez-Guerra, R., M.T. Ramírez-Rueda, M. Cabral-Enciso, M. GarcíA-Serrano, Z. Lira-Maldonado, R.G. Guevara-Gon-Zález, M. González-Chavira, J. Simpson, 2005: Heterothallic mating observed between Mexican isolates of Glomerella lindemuthiana. Mycologia 97, 793–803.

    Article  PubMed  Google Scholar 

  • Rodríguez-Guerra, R., M.T. Ramírez-Rueda, O. Martínez de la Vega, J. Simpson, 2003: Variation in genotype, pathotype and anastomosis groups of Colletotrichum lindemuthianum isolates from Mexico. Plant Pathol. 52, 228–235.

    Article  Google Scholar 

  • Rodríguez-Suárez, C., B. Méndez-Vigo, A. Pañeda, J.J. Ferreira, R. Giraldez, 2007: A genetic linkage map of Phaseolus vulgaris L. and localization of genes for specific resistance to six races of anthracnose (Colletotrichum lindemuthianum). Theor. Appl. Genet. 114, 713–722.

    Article  PubMed  Google Scholar 

  • Rohlf, J.F., 1998: Ntsys: Numerical Taxonomy and Multi-variate Analysis System. Exeter Software, New York, UsA.

  • Sicard, D., Y. Michalakis, M. Dron, C. Neema, 1997: Genetic diversity and pathogenic variation of Colletotrichum linde-muthianum in the three centres of diversity of its host Phaseo-lus vulgaris. Phytopahthology 81, 807–813.

    Article  Google Scholar 

  • Šuštar-Vozlic, J., M. Maras, B. Javornik, V. Meglic, 2006: Genetic diversity and origin of Slovene common bean (Phaseolus vulgais L.) germplasm revealed by Aflp markers and phaseolin analysis. J. Am. Soc. Hort. Sci. 131, 242–249.

    Google Scholar 

  • Tu, J.C., J.W. Aylesworth, 1980: An effective method of screening white (pea) bean seedlings (Phaseolus vulgaris L.) for resistance to Colletotrichum lindemuthianum. Phytopathol. Z. 99, 131–137.

    Article  Google Scholar 

  • Young, R.A., J.D. Kelly, 1996: Characterization of the genetic resistance to Colletotrichum lindemuthianum in common bean differential cultivars. Plant Dis. 80, 650–654.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Agricultural Institute of Slovenia, Ljubljana, Slovenia

    A. Munda & J. Šuštar-Vozlic

  2. Slovenian Institute for Hop Research and Brewing, Žalec, Slovenia

    S. Radisek

  3. Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia

    B. Javornik

Authors
  1. A. Munda
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Radisek
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Šuštar-Vozlic
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. B. Javornik
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. Munda.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Munda, A., Radisek, S., Šuštar-Vozlic, J. et al. Genetic variability of Colletotrichum lindemuthianum isolates from Slovenia and resistance of local Phaseolus vulgaris germplasm. J Plant Dis Prot 116, 23–29 (2009). https://doi.org/10.1007/BF03356282

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03356282

Key words

Stichwörter

Search

Navigation