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Occurrence of Peanut stripe virus on patchouli and raising of virus-free patchouli plants by meristem tip culture

Vorkommen des Peanut stripe virus in Indischem Patschuli und Erzeugung virusfreier Patschulipflanzen durch Meristemspitzenkultur


Patchouli (Pogostemon cablin) was found infected with a viral disease showing mosaic symptoms on the leaves in the Chand-pur farm at Institute of Himalayan Bioresource Technology campus, Palampur, Himachal Pradesh, India. After ELISA, RT-PCR, IC-PCR and sequencing, the virus was identified as Peanut stripe virus (PStV). Sequence comparison with corresponding sequences of established potyviruses showed 52–93% homol-ogy at nucleotide level. Peanut stripe virus was eliminated from Patchouli plants using meristem tip culture. MS medium amended with benzylaminopurine (2 mg l−1) and indole3-bu-tyric acid (0.05 mg l−1) was used for shoot proliferation and rooting of plants, respectively. More than 88.89% virus-free plants were obtained from 0.2 mm of meristem as shown by indirect ELISA whereas 80.55% virus-free plants were obtained from 0.3 mm size of meristem as indicated by RT-PCR.


Auf der Chandpur Farm des Institute of Himalayan Bioresour-ce Technology, Campus Palampur, Himachal Pradesh, Indien, wurde eine Virose mit Blattmosaik-Symptomen an Indischem Patschuli (Pogostemon cablin) entdeckt. Mittels ELISA, RT-PCR, IC-PCR und Gensequenzierung wurde der Erreger als Peanut stripe virus (PStV) identifiziert. Ein Sequenzvergleich mit bekannten Potyviren ergab Homologien von 52–93% auf dem Nucleotidlevel. Das Peanut stripe virus konnte mit Hilfe der Meristemspitzenkultur aus den Patschulipflanzen elimi-niert werden. Zur Spro ß - und Wurzelbildung der Calli wurde MS-Medium verwendet, dem 2 mg ml−1 Benzylaminopurin bzw. 0,05 mg ml−1 Indolbuttersäure zugegeben wurde. Mehr als 88,89% virusfreie Pflanzen wurden, wie die indirekte ELISA zeigte, aus 0,2 mm gro ß en Meristemstücken gewon-nen, während die RT-PCR ergab, dass sich aus 0,3 mm gro ß en Meristemen 80,55% virusfreie Pflanzen entwickelten.

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Singh, M.K., Chandel, V., Hallan, V. et al. Occurrence of Peanut stripe virus on patchouli and raising of virus-free patchouli plants by meristem tip culture. J Plant Dis Prot 116, 2–6 (2009).

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Key words

  • IC-PCR
  • indirect ELISA
  • meristem tip culture
  • patchouli
  • Peanut stripe virus
  • RT-PCR


  • IC-PCR
  • indirekte ELISA
  • Meristemspitzen-kultur
  • Patschuli
  • Peanut stripe virus
  • RT-PCR