Occurrence of Peanut stripe virus on patchouli and raising of virus-free patchouli plants by meristem tip culture

Vorkommen des Peanut stripe virus in Indischem Patschuli und Erzeugung virusfreier Patschulipflanzen durch Meristemspitzenkultur

Abstract

Patchouli (Pogostemon cablin) was found infected with a viral disease showing mosaic symptoms on the leaves in the Chand-pur farm at Institute of Himalayan Bioresource Technology campus, Palampur, Himachal Pradesh, India. After ELISA, RT-PCR, IC-PCR and sequencing, the virus was identified as Peanut stripe virus (PStV). Sequence comparison with corresponding sequences of established potyviruses showed 52–93% homol-ogy at nucleotide level. Peanut stripe virus was eliminated from Patchouli plants using meristem tip culture. MS medium amended with benzylaminopurine (2 mg l−1) and indole3-bu-tyric acid (0.05 mg l−1) was used for shoot proliferation and rooting of plants, respectively. More than 88.89% virus-free plants were obtained from 0.2 mm of meristem as shown by indirect ELISA whereas 80.55% virus-free plants were obtained from 0.3 mm size of meristem as indicated by RT-PCR.

Zusammenfassung

Auf der Chandpur Farm des Institute of Himalayan Bioresour-ce Technology, Campus Palampur, Himachal Pradesh, Indien, wurde eine Virose mit Blattmosaik-Symptomen an Indischem Patschuli (Pogostemon cablin) entdeckt. Mittels ELISA, RT-PCR, IC-PCR und Gensequenzierung wurde der Erreger als Peanut stripe virus (PStV) identifiziert. Ein Sequenzvergleich mit bekannten Potyviren ergab Homologien von 52–93% auf dem Nucleotidlevel. Das Peanut stripe virus konnte mit Hilfe der Meristemspitzenkultur aus den Patschulipflanzen elimi-niert werden. Zur Spro ß - und Wurzelbildung der Calli wurde MS-Medium verwendet, dem 2 mg ml−1 Benzylaminopurin bzw. 0,05 mg ml−1 Indolbuttersäure zugegeben wurde. Mehr als 88,89% virusfreie Pflanzen wurden, wie die indirekte ELISA zeigte, aus 0,2 mm gro ß en Meristemstücken gewon-nen, während die RT-PCR ergab, dass sich aus 0,3 mm gro ß en Meristemen 80,55% virusfreie Pflanzen entwickelten.

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References

  1. Anonymous, 1977: Isolation and structural analysis of pogos-tone, an antifungal component from the Chinese drug Dwang Ho Hsiang (Pogostemon cablin (Blanco) Benth). Ko Hsueeh Pao 22, 318.

    Google Scholar 

  2. Brants, D.H., H. Vermeulen, 1965: Production of virus free freesias by means of meristem culture. Eur. J. Plant Pathol. 71, 25–27.

    CAS  Google Scholar 

  3. Chenna, R., H. Sugawara, T. Koike, R. Lopez, T.J. Gibson, D.G. Higgins, J.D. THOMPSON, 2003: Multiple sequence alignment with the Clustal series of programs. Nucleic Acids Res. 31, 3497–3500.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  4. Clark, M.F., A.N. Adams, 1977: Characterization of micro plate method of enzyme linked immunosorbent assay for detection of plant viruses. J. Gen. Virol. 34, 478–483.

    Article  Google Scholar 

  5. Corpet, F., 1988: Multiple sequence alignment with hierarchical clustering. Nucleic Acids Res. 16, 10881–10890.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  6. Gomez, K.A., A.A. Gomez, 1984: Statistical Procedure for Agricultural Research. 2nd edition. John Wiley and Sons, Singapore.

    Google Scholar 

  7. Jain, R.K., I. Lahiri, A. Varma, 2000: Peanut stripe potyvirus: prevalence, detection and serological relationship. Indian Phytopathol. 53, 14–18.

    Google Scholar 

  8. Kukreja, A.K., A.K. Mathur, M. Zaim, 1990: Mass production of virus-free patchouli plants [Pogostemon cablin (Blanco) Benth.] by vitro culture. Trop. Agric. 67, 101–104.

    Google Scholar 

  9. Mckern, N.M., D.D. Shukla, O.W. Barnett, H.J. Vetten, J. Dijkstra, L.A. Whittaker, C.W. Ward, 1992: Coat protein properties suggest that Azuki bean mosaic virus, Blackeye cowpea mosaic virus, Peanut stripe virus and three strains from soybean are all strains of the same potyvirus. Intervi-rology 33, 121–134

    CAS  Google Scholar 

  10. Murashige, T., F.F. Skoog, 1962: Revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 15, 473–497.

    CAS  Article  Google Scholar 

  11. Page, R.D.M., 1996: TreevIEW–An application to display phylogenetic trees on personal computers. Comp. Appl. Biosci. 12, 357–358.

    CAS  PubMed  Google Scholar 

  12. Rao, R.D., V.J. Chakrabarty, A.S. Reddy, 1989: Peanut stripe virus occurrence in India. Indian Phytopathol. 42, 487–491.

    Google Scholar 

  13. Saiz, M., J. Dopazo, S. Castro, J. Romero, 1994: Evolutionary relationships among BCMV strains and closely related potyviruses. Virus Res. 31, 39–48.

    CAS  Article  PubMed  Google Scholar 

  14. Sanger, F., S. Nickelen, A.R. Coulson, 1977: DnA sequencing with chain termination inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463–5467.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  15. Sharma, R., 2002: Medicinal Plants of India- an Encylopaedia, pp. 197–198. Daya Publishing House, New Delhi.

    Google Scholar 

  16. Sugimura, Y., M.S. Padayhag, K.N. Ceniza, S. Eguchi, T. Natsuaki, S. Okuda, 1995: Essential oil production increased by using virus free patchouli plants derived from meristem-tip culture. Plant Pathol. 44, 510–515.

    Article  Google Scholar 

  17. Tatusova, A. T., T.L. Maiden, 1999: Blast 2 sequences. A new tool for comparing protein and nucleotide sequences. FEMS Microbiol. Lett. 174, 247–250.

    CAS  Article  PubMed  Google Scholar 

  18. Van der Vlugt, R.A.A., P. Steffens, C. Cuperus, E. Barg, D.E. Lesemann, L. Bos, H.J. Vetten, 1999: Further evidence that Shallot yellow stripe virus (SYSV) is a distinct potyvirus and reidentification of Welsh onion stripe virus as a Sysv strain. Phytopathology 89, 148–155.

    Article  PubMed  Google Scholar 

  19. Vetten, H.J., D.E. Lesemann, E. Maiss, 1992: Serotype A and B strains of BCMV are two distinct potyviruses. In: O.W. Barnett (ed.): Potyvirus Taxonomy, pp. 415–431. Springer, Wien, NewYork.

    Chapter  Google Scholar 

  20. Xu, Z., Z. Yu, J. Lui, O.W. Barnett, 1983: Virus causing peanut mild mottle in Hubei province, China Plant Dis. 67, 1029–1032.

    Article  Google Scholar 

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Correspondence to A. A. Zaidi.

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Singh, M.K., Chandel, V., Hallan, V. et al. Occurrence of Peanut stripe virus on patchouli and raising of virus-free patchouli plants by meristem tip culture. J Plant Dis Prot 116, 2–6 (2009). https://doi.org/10.1007/BF03356278

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Key words

  • IC-PCR
  • indirect ELISA
  • meristem tip culture
  • patchouli
  • Peanut stripe virus
  • RT-PCR

Stichwörter

  • IC-PCR
  • indirekte ELISA
  • Meristemspitzen-kultur
  • Patschuli
  • Peanut stripe virus
  • RT-PCR