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Susceptibility of Greek alfalfa and clover cultivars to Fusarium oxysporum f. sp. medicaginis and potential methods of disease control

Anfälligkeit griechischer Luzerne- und Kleesorten gegenüber Fusarium oxysporum f. sp. medicaginis und potenzielle Bekämpfungsverfahren

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Abstract

The fungus Fusarium oxysporum f. sp. medicaginis (Fom), as verified following Koch’s postulates, was isolated from diseased alfalfa plants, cv. Yliki, in Greece. The susceptibility of eight most cultivated in Greece alfalfa and two clover cultivars was tested using seedlings that were artificially inoculated according to the root-dipping method. All alfalfa cultivars were susceptible to Fom, while the fungus was non pathogenic to clover cultivars. The effectiveness of the biological agents 4F3 (nit mutant of non-pathogenic F. oxysporum isolate) and 618–12B17 (non-pathogenic benomyl resistant F. oxysporum mutant), the chemical substances ß-aminobutyric acid and Bion and fungicide carbendazim was evaluated regarding disease control. Disease incidence was suppressed in association with carbendazim, 4F3, 618–12B17 and Bion treatments.

Zusammenfassung

Der anhand von Koch’s Postulaten identifizierte Pilz Fusarium oxysporum f. sp. medicaginis (Fom) wurde aus infizierten Luzernepflanzen der Sorte Yliki in Griechenland isoliert. Die Anfälligkeit der acht verbreitetsten Luzernesorten Griechenlands und zweier Kleesorten gegenüber dem Pilz wurde mit der Wurzeltauchmethode untersucht. Während alle Luzernesorten anfällig gegenüber Fom waren, war der Pilz gegenüber den beiden Kleesorten nicht pathogen. Die Effizienz der Antagonisten 4F3 (nit-Mutante eines nicht-pathogenen F.-oxyspo- rum-Isolats) und 618-12B17 (nicht-pathogene Benomyl-resis- tente F.-oxysporum-Mutante), der chemischen Substanzen ß-Aminobuttersäure und Bion sowie des Fungizids Carbenda- zim wurde hinsichtlich einer Kontrolle der Erkrankung untersucht. Die Befallshäufigkeit sank gegenüber der Kontrolle nach Behandlungen mit 4F3, 618–12B17 und Bion.

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Literature

  • Alabouvette, C., Y. Couteaudier, 1992: Biological control of Fusarium wilt with nonpathogenic Fusaria. In: E.C. Tjamos, G. C. Papavizas, R.J. Cook (eds.): Biological Control of Plant Diseases, pp. 415–426. Plenum Press, New York, USA.

    Chapter  Google Scholar 

  • Anfoka, F.H., 2000: Benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester induces systemic resistance in tomato (Lycopersicon esculentum Mill. cv. Vollendung) to Cucumber Mosaic Virus. Crop Prot. 19, 401–405.

    Article  CAS  Google Scholar 

  • Antonopoulos, D.F., K. Elena, E.C. Tjamos, 2003: First report of Fusarium wilt of alfalfa in Greece. Plant Dis. 87, 751.

    Article  CAS  Google Scholar 

  • Armstrong, G.M., J.K. Armstrong, 1954: Alfalfa-A common host for the wilt Fusaria from alfalfa, cotton and cassia. Plant Dis. Rep. 38, 221–222.

    Google Scholar 

  • Armstrong, G.M., J.K. Armstrong, 1965: Further studies on the pathogenicity of three forms of Fusarium oxysporum causing wilt of alfalfa. Plant Dis. Rep. 49, 412–416.

    Google Scholar 

  • Bao, J.R., G. Lazarovits, 2001: Differential colonization of tomato roots by nonpathogenic and pathogenic Fusarium oxysporum strains may influence Fusarium wilt control. Phytopathology 91, 449–456.

    Article  CAS  Google Scholar 

  • Benhamou, N., R.R. Belanger, 1998: Benzothiadazole-mediat- ed induced resistance to Fusarium oxysporum f. sp. radicis-ly- copersici in tomato. Plant Physiol. 118, 1203–1212.

    Article  CAS  Google Scholar 

  • Campbell, C.L., L.V. Madden, 1990: Introduction to Plant Disease Epidemiology. John Wiley & Sons Inc., New York.

    Google Scholar 

  • Cohen, Y., 1994: ß-aminobutyric acid induces systemic resistance against Peronospora tabacina. Physiol. Mol. Plant Pathol. 44, 273–288.

    Article  CAS  Google Scholar 

  • Cole, D.L., 1999: The efficacy of acibenzolar-S-methyl, an inducer of systemic acquired resistance, against bacterial and fungal diseases of tobacco. Crop Prot. 18, 267–273.

    Article  CAS  Google Scholar 

  • Corell, J.C., C.J.R. Klittich, J.F. Leslie, 1987: Nitrate nonutilizing mutants of Fusarium oxysporum and their use in vegetative compatibility tests. Phytopathology 77, 1640–1644.

    Article  Google Scholar 

  • Davidse, L.C., 1986: Benzimidazole fungicides: mechanisms of action and biological impact. Annu. Rev. Phytopathol. 24, 43–65.

    Article  CAS  Google Scholar 

  • Elena, K., E.J. Paplomatas, C. Lampropoulos, 2001: Biological control of Fusarium oxysporum f. sp. dianthi using nonpathogenic strains of F. osysporum. Proceedings, Ninth Hellenic Phytopathological Conference, Athens Greece 20–22 October 1998: Phytopathol. Medit. 40, 93.

    Google Scholar 

  • Emberger, G., R.E. Welty, 1983: Evaluation of virulence of Fusarium oxysporum f. sp. medicaginis and Fusarium Wilt resistance in alfalfa. Plant Dis. 67, 94–98.

    Article  Google Scholar 

  • Erwin, D.C., 1990: Fusarium wilt. In: D. Studevill, D.C. Erwin (eds.): Compendium of alfalfa diseases, pp 33–34. Second edition. Phytopathological Society, St. Paul, MN, USA.

    Google Scholar 

  • Frosheiser, F.I., D.K. Barnes, 1978: Field reaction of artificially inoculated alfalfa populations to the Fusarium and Bacterial wilt pathogens alone and in combination. Phytopathology 68, 943–946.

    Article  Google Scholar 

  • Garibaldi, A., F. Brunatti, M.L. Gullino, 1987: Evaluation of several antagonists and different methods of application against Fusarium wilt of Carnation. EPPO Bull. 17, 625–629.

    Article  Google Scholar 

  • He, C.Y., T. Hsiang, D.J. Wolyn, 2002: Induction of systemic disease resistance and pathogen defense responses in Asparagus officinalis inoculated with nonpathogenic strains of Fusarium oxysporum. Plant Pathol. 51, 225–230.

    Article  Google Scholar 

  • JENKINS, J.H., G.D. LINDBERG, 1961: Fusarium wilt of alfalfa in Louisiana. Southern Division Report and Abstracts, 643.

  • Kessmann, H., T. Stau, C. Hofman, T. Maetzke, J. Herttzog, E. Ward, S. Uknes, J. Ryals, 1994: Induction of systemic acquired disease resistance in plants by chemicals. Annu. Rev. Phytopathol. 32, 439–459.

    Article  CAS  Google Scholar 

  • Korolev, N., E. Pèrez-Artèz, J. Bejarano-Alcázar, D. Rodríguez-Jurado, J. Katan, T. Katan, R.M. Jimènez-Duípaz, 2001: Comparative study of genetic diversity and pathogenicity among populations of Verticillium dahliae from cotton in Spain and israel. Eur. J. Plant Pathol. 107, 443–456.

    Article  CAS  Google Scholar 

  • Larkin, R.P., D.R. Fravel, 1999: Field efficacy of selected non-pathogenic Fusarium spp. and other biocontrol agents for control of Fusarium wilt of tomato. Biol. Cult. Tests 14, 116.

    Google Scholar 

  • Larkin, R.P., D.R. Fravel, 2002: Effects of varying environmental conditions on biological control of Fusarium wilt of tomato by non-pathogenic Fusarium spp. Phytopathology 92, 1160–1166.

    Article  Google Scholar 

  • Lawton, K.A., L. Friedrich, M. Hunt, K. Weymann, T. Delaney, H. Kessmann, T. Staub, J.A. Ryals, 1996: Benzothiadazole induces disease resistance in Arabidopsis by activation of the systemic acquired resistance signal transduction pathway. Plant J. 10, 71–82.

    Article  CAS  Google Scholar 

  • Leath, K.T., 1993: Interaction of Fusarium oxysporum f. sp. medicaginis with feeding activity of clover root curculio larvae in alfalfa. Plant Dis. 77, 799–802.

    Article  Google Scholar 

  • Mauch-Mani, B., J.-P. Métraux, 1998: Salicylic acid and systemic acquired resistance to pathogen attack. Ann. Bot. 82, 535–540.

    Article  CAS  Google Scholar 

  • Oostendorp, M., W. Kunz, B. Dietrich, T. Syaub, 2001: Induced disease resistance in plants by chemicals. Eur. J. Plant Pathol. 107, 19–28.

    Article  CAS  Google Scholar 

  • Postma, J., H. Rattink, 1992: Biological control of Fusarium wilt of carnation with nonpathogenic isolate of Fusarium oxysporum. Can. J. Bot. 70, 1199–1205.

    Article  Google Scholar 

  • Puhalla, J.E., 1985: Classification of strains of Fusarium oxysporum on the basis of vegetative compatibility. Can. J. Bot. 63, 179–183.

    Article  Google Scholar 

  • Rouxe, F., D. Bouhot, 1971: Recherches sur l’ ecology des champignons parasites dans le sool. IV. Nouvelles mises au point concernant l’ analyse sélective et quantitative des Fusarium oxysporum et F. solani dans le sol. Ann. Phytopathol. 3, 171–188.

    Google Scholar 

  • Siegrist, J., M. Orober, H. Buchenauer, 2000: ß-aminobutyric acid-mediated enhancement of resistance in tobacco to tobacco mosaic virus depends on the accumulation of salicylic acid. Physiol. Mol. Plant Pathol. 56, 95–106.

    Article  CAS  Google Scholar 

  • Sinha, A.K., R.K.S. Wood, 1968: Studies on the nature of resistance in tomato plants to Verticillium albo-atrum. Ann. Appl. Biol. 62, 319–327.

    Article  Google Scholar 

  • Snyder, W.C., H.N. Hansen, 1940: The species concept in Fusarium. Am. J. Bot. 27, 65–67.

    Article  Google Scholar 

  • Stephens, C.T., W.H. Elmer, 1988: Comparison of techniques for eliminating contaminants from asparagus seeds. HortScience 23, 1031–1032.

    Google Scholar 

  • Sticher, L., B. Mach-Mani, J.-P. Métraux, 1997: Systemic acquired resistance. Annu. Rev. Phytopathol. 35, 235–270.

    Article  CAS  Google Scholar 

  • Tamietti, G., R. Pramotton, 1987: Une méthode très simple de sélection de souches de Fusarium spp. non pathogenes, antagonistes des fusarioses vasculaires. EPPO Bull. 17, 549–557.

    Article  Google Scholar 

  • Weimer, J.L., 1928: A wilt disease of alfalfa caused by Fusarium oxysporum var. medicaginis. J. Agric. Res. 37, 419–433.

    Google Scholar 

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  1. Laboratory of Mycology, Benaki Phytopathological Institute, 8 St. Delta, Kifissia, 14 561, Greece

    D. F. Antonopoulos & K. Elena

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  1. D. F. Antonopoulos
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  2. K. Elena
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Correspondence to D. F. Antonopoulos.

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Antonopoulos, D.F., Elena, K. Susceptibility of Greek alfalfa and clover cultivars to Fusarium oxysporum f. sp. medicaginis and potential methods of disease control. J Plant Dis Prot 115, 162–166 (2008). https://doi.org/10.1007/BF03356260

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  • DOI: https://doi.org/10.1007/BF03356260

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