Abstract
ß-aminobutyric acid (BABA) is a simple, nonprotein amino acid that exhibits postinfectional activity against nematodes, fungi, bacteria and tobacco mosaic virus (TMV), but its activity against phytoplasmas has never been examined before. The aim of this research was to examine possible postinfectional activity of BABA against phytoplasma reference strains AY-A and KVI in Catharanthus roseus shoots grown in vitro. Shoots were grown on Murashige and Skoog (MS) medium supplemented with different concentrations of BABA (10 μM, 100 μM, 1 mM, 10 mM, 100mM), through five subcultures. The length of the shoots infected with phytoplasma strain AY-A was measured at the beginning and at the end of the first three subcultures. Only in the first subculture BABA supported shoot elongation. The presence of phytoplasmas was confirmed by nested PCR in both AY-A and KVI-infected shoots after the first and the fifth subculture, showing that in these experimental conditions BABA had no curative effect against phytoplasmas.
Zusammenfassung
ß-Aminobuttersäure (BABA) ist eine einfache nicht proteinogene Aminosäure mit postinfektioneller Aktivität gegenüber Nematoden, Pilzen, Bakterien und dem Tabakmosaikvirus (TMV), aber ihre Wirkung gegenüber Phytoplasmen wurde bisher nicht untersucht. Das Ziel dieser Untersuchung war die überprüfung der möglichen postinfektionellen Aktivität gegenüber den Phytoplasmen-Referenz-stämmen AY-A und KVI in in-vitro-Sprosskulturen von Catharanthus roseus. Die Sprosse wurden in Murashige-Skoog-Medium (MS) in Gegenwart unterschiedlicher BABA-Konzentrationen (10 μM, 100 μM, 1 mM, 10 mM, 100 mM) fünfmal subkultiviert. Die Länge der mit dem Phytoplasmenstamm AY-A infizierten Sprosse wurde zu Beginn und am Ende der ersten drei Subkulturen gemessen. BABA verstärkte das Sprosswachstum nur in der ersten Subkultur. Phytoplasmen beider Stämme wurden mit Hilfe der nested PCR nach der ersten und der letzten Subkultivierung nachgewiesen, so dass BABA unter den gewählten experimentellen Bedingungen keinen kurativen Effekt gegenüber Phytoplasmen aufwies.
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Ćurković Perica, M., Šeruga Musić, M. Effect of β-aminobutyric acid on phytoplasma-infected Catharan-thus roseus shoots. J Plant Dis Prot 112, 544–549 (2005). https://doi.org/10.1007/BF03356151
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DOI: https://doi.org/10.1007/BF03356151