Abstract
The axillary bud-break and multiple bud induction were obtained from the nodal explants of field-grown culms of Bambusa tulda in liquid Murashige and Skoog’s (MS) basal medium supplemented with 2.0 mg l−1 6-benzylaminopurine (BAP), 1.0 mg l−1 kinetin (Kn) and 8% coconut water. Multiple shoots regenerated and proliferated in the liquid MS medium fortified with 3.0 mg l−1 indolebutyric acid (IBA). While, in B. balcooa, MS medium supplemented with 2.5 mg l−1 BAP and 1.0 mg l−1 Kn induced axillary bud-break, bud multiplication and subsequently shoot elongation was obtained after three passages in the same medium. A clump with at least three shoots of both these bamboo species was used as propagule for successful root induction in half-strength MS liquid basal medium supplemented with 0.2 mg l−1 IBA. Sympodial type of microrhizomes developed in B. tulda and the regenerants acclimatized in the soil easily. Explants collected in the month of October produced best in vitro regeneration response in these two bamboo species. Endogenous phenol content proved detrimental for efficient shoot regeneration. The clonal fidelity of the regenerants was established by RAPD analysis advocating clonal propagation through axillary meristem culture of B. balcooa and B. tulda is reliable for commercial exploitation.
References
Bhatt BP, Singha LB, Singh K & Sachan MS, Bamboo Sci Cult, 17 (2003) 4.
Dransfield S & Widjaja EA, Plant resources of South-East Asia No 7. Bamboos, Backhuys Publishers, Leiden (1995).
Upreti TC & Sundriyal RC, Bamboo Sci Cult, 15 (2001) 20.
Sharma YML, In Recent research in bamboo (AN Rao, G Dhanarajan, CB Sastry, Editors), Proceedings of the International Bamboo Workshop, Hangzhou, China (1987) pp 14–17.
Saxena S, Plant Cell Rep, 9 (1990) 431.
Shenoy VB & Vasil IK, Theor Appl Genet, 83 (1992) 947.
Leory XJ, Leon K, Charles C & Branchard M, Plant Cell Rep, 19 (2000) 1102.
Gielis J, Peeters H, Gillis K, Oprins J & Debergh PC, In Strategies for new ornamentals (J van Huylebrock, E van Bockstaele, P Debergh, Editors) Proceedings of the 20th International Eucarpia Symposium (2002) pp 195–203.
Varshney A, Lakshmikumaran M, Srivastava PS & Dhawan V, In Vitro Cell Dev Biol Plant, 37 (2001) 227.
Murashige T & Skoog F, Physiol Plant, 15 (1962) 473.
Das M & Pal A, Plant Cell Tiss Org Cult, 81 (2005) 109.
Basak J, Das M, Ghose TK & Pal A, In Biotechnology for a better future (L D’souza, M Anuradha, N Shashikiran, S Hegde, K Rajendra, Editors) SAC Publications, Mangalore (2004) pp 99–105.
Ramanayake SMSD & Yakandawal K, Plant Sci, 129 (1997) 213.
Chowdhury P, Das M, Sikdar SR & Pal A, Plant Cell Biotech Mol Biol, 5 (2004) 45.
Lin CS & Chang WC, Plant Cell Rep, 17 (1998) 617.
Schoofs J, In Rapid propagation of fast growing woody species (FWG Baker, Editor) CAB International, Oxon, UK (1992) pp 29–40.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Das, M., Pal, A. Clonal Propagation and Production of Genetically Uniform Regenerants from Axillary Meristems of Adult Bamboo. J. Plant Biochem. Biotechnol. 14, 185–188 (2005). https://doi.org/10.1007/BF03355956
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF03355956