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Nano-Micro Letters

, Volume 5, Issue 3, pp 213–222 | Cite as

One Step Quick Detection of Cancer Cell Surface Marker by Integrated NiFe-based Magnetic Biosensing Cell Cultural Chip

  • Chenchen Bao
  • Lei Chen
  • Tao Wang
  • Chong Lei
  • Furong Tian
  • Daxiang CuiEmail author
  • Yong Zhou
Open Access
Article

Abstract

RGD peptides has been used to detect cell surface integrin and direct clinical effective therapeutic drug selection. Herein we report that a quick one step detection of cell surface marker that was realized by a specially designed NiFe-based magnetic biosensing cell chip combined with functionalized magnetic nanoparticles. Magnetic nanoparticles with 20-30 nm in diameter were prepared by coprecipitation and modified with RGD-4C, and the resultant RGD-functionalized magnetic nanoparticles were used for targeting cancer cells cultured on the NiFe-based magnetic biosensing chip and distinguish the amount of cell surface receptor-integrin. Cell lines such as Calu3, Hela, A549, CaFbr, HEK293 and HUVEC exhibiting different integrin expression were chosen as test samples. Calu3, Hela, HEK293 and HUVEC cells were successfully identified. This approach has advantages in the qualitative screening test. Compared with traditional method, it is fast, sensitive, low cost, easy-operative, and needs very little human intervention. The novel method has great potential in applications such as fast clinical cell surface marker detection, and diagnosis of early cancer, and can be easily extended to other biomedical applications based on molecular recognition.

Keywords

Magnetic impedance Cancer cell Targeted detection Biosensing system Cell cultural chip 

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Copyright information

© Shanghai Jiao Tong University (SJTU) Press 2010

Authors and Affiliations

  • Chenchen Bao
    • 1
  • Lei Chen
    • 1
  • Tao Wang
    • 1
  • Chong Lei
    • 1
  • Furong Tian
    • 2
  • Daxiang Cui
    • 1
    Email author
  • Yong Zhou
    • 1
  1. 1.Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Research Institute of Micro/Nano Science and TechnologyShanghai Jiao Tong UniversityShanghaiChina
  2. 2.FOCAS Research InstituteDublin Institute of TechnologyDublin 8Ireland

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