Abstract
The adenylate cyclase-cyclic AMP-phosphodiesterase system of human thyroid tissues adjacent to cold nodules (control), two follicular adenomas, one hyperplastic thyroid and one hyperfunctioning follicular carcinoma have been compared. In the hyperfunctional follicular carcinoma the basal adenylate cyclase is much higherthan in control tissue; carcinoma adenylate cyclase does not respond to TSH and prostaglandin E1, whereas it responds normally to fluoride. In the hyperplastic, but hypofunctional thyroid the basal adenylate cyclase is higher than in normal tissue whereas the response to TSH, PGE1, and fluoride is normal. No difference between the follicular adenomas and normal thyroid stimulated and unstimulated adenylate cyclase was observed. Furthermore in various thyroid tissues no changes in the level of cyclic AMP phosphodiesterase was found. Our data indicate a greater change in the synthesis rather than in degradation of cyclic AMP in the human pathological thyroids studied.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Haran-Ghera N., Pullar R., Furth, J. Induction of thyrotropin dependent thyroid tumors by thyrotropes. Endocrinology 66: 694, 1960.
Isler H. Effects of iodine on thyroid tumors induced in the rat by a low iodine diet. J. Natl. Cancer Inst. 23: 675, 1959.
Wollman S.H. Effects of feeding thiouracil on thyroid gland of rats. J. Natl. Cancer Inst. 26: 473, 1961.
Wollman S.H. Production and properties of transplantable tumors of thyroid gland in the Fisher rats. Recent Prog. Horm. Res. 19: 579, 1963.
Pastan I., Katzen R. Activation of adenyl-cyclase in thyroid homogenates by thyroid stimulating hormone. Biochem. Biophys. Res. Comm. 29: 792, 1967.
Macchia V., Meldolesi M.F., Maselli P. Effect of cyclic 3′, 5′-AMP on glucose metabolism in thyroid homogenates. Endocrinology 85: 895, 1969.
Macchia V., Tenore A., Varrone S. Metabolic activities of thyroid gland from theophylline treated rats. Exp. Mol. Pathol. 17: 94, 1972.
Peery C.V., Johnson G.S., Pastan I. Adenyl-cyclase in normal and trasformed fibroblasts in tissue culture. J. Biol. Chem. 246: 5785, 1971.
Macchia V., Meldolesi M.F., Chiariello M. Adenyl-cyclase in a transplantable thyroid tumor-loss of ability to respond to TSH. Endocrinology 90: 1483, 1972.
Mandato E., Meldolesi M.F., Macchia V. Diminished binding of thyroid-stimulating hormone in a transplantable rat thyroid tumor as a possible cause of hormone unresponsiveness. Cancer Res. 35: 3089, 1975.
Krishna G.B., Weiss B., Brodie R. A simple sensitive method for the assay of adenyl-cyclase. J. Pharmacol. Exp. Ther. 163: 379, 1968.
White A.A., Zenzer T.V. Separation of cyclic 3′, 5′ nucleotide monophosphates from other nucleotides on aluminum oxide columns. Application to the assay of adenyl-cyclase and guanyl cyclase. Anal. Biochem. 11: 372, 1971.
Giannattasio M., Macchia V. Adenylate cyclase and cyclic 3′–5′ AMPdiesterase in Jerusalem artichoke tubers. Plant Sci. Lett. 1: 259, 1973.
Lowry O.H., Rosebrough N.J., Farr A.L, Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265, 1951.
Verrier B., Fayet G., Lissitzy S. Thyrotropin binding properties of isolated thyroid cells and their purified plasma membrane. Eur. J. Biochem. 42: 355, 1974.
Wolff J., Jones A.B. The purification of bovine thyroid plasma membranes and the properties of membranes bound adenyl cyclase. J. Biol. Chem. 246: 3939, 1971.
Meldolesi M.F., Fishman P.H., Aloj S.M., Kohn L.D., Brady R.O. Relationship of gangliosides to the structure and function of thyrotropin receptors: their absence on plasma membranes of a thyroid tumor defective in thyrotropin receptor activity Proc. Natl. Acad. Sci. USA 73: 4060, 1976.
Macchia V., Wolff J. The effect of a purified preparation of lecithinase on iodine preparations. Febs Lett. 10: 219, 1970.
De Rubertis F., Yamashita K., Dekker A., Larsen R., Field J. Effects of TSH on adenyl cyclase activity and intermediary metabolism of cold thyroid nodules and normal human thyroid tissue. J. Clin. Invest. 51: 1109, 1972.
Orgiazzi J., Munari Y., Rastegnat A., Morney R. Supranormal sensitivity of adenylate cyclase from toxic thyroid nodules. 11 th Ann. Meet. Europ. Soc. Clin. Invest. 1977, Rotterdam, Abst. no. 153.
Field J.B., Larsen P.R., Kotani M., Kariya T., Kerins M., Bloom G. Biochemical heterogeneity and diminished TSH responsiveness in human thyroid carcinoma. In: Robbins J., Braverman L.E. (Eds.), Thyroid Research. Excerpta Medica, Amsterdam, 1975, p. 551.
Sand G., Jortay A., Pochet R., Dumont J. Adenylate cyclase and protein Phosphokinase activities in human thyroid. Comparison of normal glands, hyper-functional nodules and carcinomas. Eur. J. Cancer. 12: 447, 1976.
Abe Y., Ichikawa Y., Homma M., Ito K., Mimura T. TSH receptor and adenylate cyclase in undifferentiated thyroid carcinoma. Lancet 2: 506, 1977.
Macchia V., Meldolesi M.F., Mandato E. Alterations in TSH receptor in two transplantable rat thyroid tumors. Ann. Radiol. (Paris) 20: 695, 1977.
Takaso N., Stato S., Yamada T, Makiuchi M., Furihata R., Miyata, M. The different modes of action of TSH and PGEi on cyclic AMP synthesis in human thyroid, as studied by sequential stimulations. Horm. Metab. Res. 8: 206, 1976.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Macchia, V., Mandato, E., Carella, C. et al. The adenylate cyclase-cyclic AMP-phosphodiesterase system in pathological human thyroid. J Endocrinol Invest 1, 337–345 (1978). https://doi.org/10.1007/BF03350979
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF03350979