Abstract
LATS containing sera and a number of Graves’ disease sera stimulated T4 release from mouse thyroids in vitro as, determined by RIA, thus confirming the presence of a thyroid hormone releasing factor in sera of thyrotoxic patients. The pattern of stimulation was similar to that previously shown for TSH in terms of T4 release time sequence, cAMP increase and catecholamine inhibition via or-adrenergic receptors. In the same in vitro system, neutralization with a human thyroid homogenate showed presence of LATS-Protector (LPA) in LATS negative thyrotoxic sera. The present study describes a simpler procedure for estimating LATS or similar activity, as compared to the McKenzie assay, and suggests identical receptor sites for TSH and other thyroid stimulators.
Similar content being viewed by others
References
McKenzie J.M. Long acting thyroid stimulator of Graves’ disease. In: Greer M.A. and Solomon D.H., (Eds), Handbook of Physiology, Endocrinology III. The Thyroid. Am. Physiol. Soc. Wash., D.C., p. 285, 1974.
Gafni M., Sirkis N., Gross J. Inhibition of the response of mouse thyroid to thyrotropin induced by chronic triiodothyronine treatment. Endocrinology 97: 1256, 1975.
Brown J., Munro D.S. A new in vitro assay for thyroid stimulating hormone. J. Endocrinol. 38: 439, 1967.
Williams JA, Wolff J. Thyroid secretion in vitro: multiple actions of agents affecting secretion. Endocrinology 88: 206, 1971.
Maayan M.L., Debons A.F., Krimsky I., Volpert E.M., From A., Dawry F., Siclari E. Inhibition of thyrotropin and dibutyryl cyclic AMP induced secretion of thyroxine and triiodothyronine by catecholamines. Endocrinology 101: 284, 1977.
Adams D.D., Kennedy T.H. Occurrence in thyrotoxicosis of a gamma globulin which protects LATS from neutralization by an extract of thyroid gland. J. Clin. Endocrinol. Metab. 27: 173, 1970.
Solomon D.H., Chopra I.J., Chopra V., Smith F.J. Identification of sub-groups of euthyroid Graves’ ophthalmopathy. N. Engl. J. Med. 296: 181, 1977.
Knox A.J.S., VonWestarp C., Row V.V., Volpé R. Thyroid antigen stimulates lymphocytes from patients with Graves’ disease to produce thyroid stimulating immunoglobulins. J. Clin. Endocrinol. Metab. 43: 330, 1976.
Volpert E.M. Iodoamino acids in normal and iodine-deficient rat thyroids; comparison between 125l and 127l distribution. Endocrinology 93: 1241, 1973.
Wood L.C., Burger A., Peterson M., Ingbar S.H. Induction of a LATS-like response by thyroglobulin in the McKenzie assay system. Endocrinology 92: 1538, 1973.
Wolff J., Cook G.H. Charge effects in the activation of adenylate cyclase. J. Biol. Chem. 250: 6897, 1975.
Hall R., Smith B.R., Mukhtar E.D. Thyroid stimulators in health and disease. Clin. Endocrinol. (Oxf.) 4: 213, 1975.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Maayan, M.L., Volpert, E.M. & Dawry, F.P. An in vitro procedure for the estimation of thyroid hormone releasing factors in sera of thyrotoxic patients. J Endocrinol Invest 1, 299–304 (1978). https://doi.org/10.1007/BF03350973
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF03350973