Abstract
Trypan blue was previously shown to directly inhibit thyroid secretion following TSH stimulation. Inhibition of both colloid droplet formation and thyroglobulin proteolysis was demonstrated. By observing the characteristic bright red fluorescence of the dye-protein complex, we have demonstrated that trypan blue rapidly enters the colloid space and combines with thyroglobulin. In addition, the dye in association with thyroglobulin has been demonstrated within phagosomes and phagolysosomes by centrifugation of the lysosomal (P15) fraction on both sucrose and Percoll density gradients. Lability or latency of the dye with the phagolysosomal contents was demonstrated and the dye was found in association with thyroglobulin by column chromatography. It is proposed that the complexing of trypan blue to thyroglobulin alters its attachment to specific follicular cell receptors, inhibits pinocytosis, and, thus, thyroid hormone secretion.
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Peake R.L., Battle C.L., Harrison R.L., Payer A.F. Direct effects of trypan blue on thyroid secretion. Horm. Res. 10:191, 1979.
Battle C.L., Payer A.F., Peake R.L. Effects of trypan blue on thyroid secretion Inhibition of purified cathepsin D from bovine thyroid. Biochim. Biophys. Acta 660:8, 1981.
Payer A.F., Battle C.L., Peake R.L Ultrastructural and cytochemical effects of trypan blue on TSH stimulation of thyroid follicular cells. Cell tissue Res. 218:547, 1981.
Lloyd J.B., Beck F., Griffiths A., Parry I.M. The mechanism of action of acid bisazo dyes In: Campbell P.N. (Ed.), The interaction of drugs and subcellular components in animal cells. Little Brown and Co., Boston, 1968, p. 171.
Davis H.W., Sauter R.W. Fluorescence of trypan blue in frozen-dried embryos of the rat. Histochemistry 54:177, 1977.
Williams J.A., Wolff J. Thyroid secretion in vitro: Multiple actions of agents affecting secretion. Endocrinology 88:206, 1971.
Balasubramaniam K., Deiss W.P., Jr. Characteristics of thyroid lysosomal cathepsin. Biochim. Biophys. Acta 7 70:564, 1965.
Peake R.L., Cates R.J., Deiss W.P., Jr. Thyroglobulin degradation: particulate intermediates produced in vivo. Endocrinology 87:494, 1970.
Bailey J.L Estimation of protein Techniques in protein chemistry, 2nd Ed., Elsevier, Amsterdam, 1967, p. 340.
De Duve C. Lysosomes, a new group of cytoplasmic particles In: Hayashi T. (Ed.), Subcellular particles. Ronald Press, New York, 1959, p. 128.
Pertoft H., Laurent T.C. Isopycnic separation of cells and cell organelles by centrifugation in modified colloidal silica gradients. In: Catsimpoolas N., (Ed.), Methods of cell separation. Plenum Press, New York, 1977, Vol. 1, p. 53.
Callebaut M., Harrisson F., Vakaet L. Peripheral avian yolk assemblage and its persistance in the blastoderm, studied by trypan blue-induced fluorescence. Anat. Embryol. (Berl.) 163:55, 1981.
Hamberger A., Hamberger B. Uptake of catecholamines and penetration of trypan blue after blood-brain barrier lesions. Z. Zeilforsch. 70:386, 1966.
Steinwall O., Klatzo I. Selective vulnerability of the blood-brain barrier in chemically induced lesions. J. Neuropathol. Exp. Neurol. 25:542, 1966.
Ericson L.E. Exocytosis and endocytosis in the thyroid follicle cell. Mol. Cell. Endocrinol. 22:1, 1981.
Seljelid R. Endocytosis in thyroid follicle cells III. An electron microscopic study of the cell surface and related structures. J. Ultrastruct. Res. 18:1, 1967.
Seljelid R. Endocytosis in thyroid follicle cells IV. On the acid phosphatase activity in thyroid follicle cells, with special reference to the quantitative aspects. J. Ultrastruct. Res. 18:237, 1967.
Wattiaux R., De Duve C. Release of bound hydrolases by means of Triton X-100. Biochem. J. 63:606, 1956.
Hashizume K., Fenzi G.F., DeGroot L. Thyroglobulin inhibition of thyrotropin binding to thyroid plasma membrane. J. Clin. Endocrinol.Metab. 46:679, 1978.
Consiglio E., Salvatore G., Rall J., Kohn L Thyroglobulin interactions with thyroid plasma membranes. J. Biol. Chem. 254:5065, 1979.
Williams K., Roberts G., Kidston M.E., Beck F., Lloyd J. Inhibition of pinocytosis in rat yolk sac by trypan blue. Teratology 14:343, 1976.
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Peake, R.L., Payer, A.F. & Battle, C.L. Effects of trypan blue on thyroid secretion: localization of trypan blue within the colloid space and phagolysosomes of thyroid follicles. J Endocrinol Invest 6, 161–167 (1983). https://doi.org/10.1007/BF03350601
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DOI: https://doi.org/10.1007/BF03350601