Abstract
Biliary excretion of thyroxine (T4), 3,5,3’-triiodothyronine (T3), 3,3,5’-triiodothyronine (rT3) and diiodothyronines (3, 3’-T2, 3, 5-T2 and 3’, 5’-T2) was estimated with the aid of radioimmunoassay in 3–4 subsequent 2-h samples of bile obtained from pentobarbital anesthetized rats through the tubing inserted in bile duct. The excretion of T3 was significantly decreased during 4-h infusion of 2400 ng/kg/min adrenaline in normal rats or during 6-h infusion of the latter dose in the animals preinjected with 2 µg T4. Moreover, the excretion of rT3 was significantly increased after the infusion of 1200 and 2400 ng/kg/min adrenaline. Such increase after 1200 and 2400 ng/kg/min adrenaline was prevented by a single dose of 10 mg/kg phentolamine (alpha1–2-antagonist) and that after 2400 ng/kg/min adrenaline also by 2.5 mg/kg prazosin (alpha1-antagonist) injected at the beginning of the infusion, but not by 6 mg/kg yohimbine (alpha2-antagonist) injected every 60 min during 4-h infusion. In addition, increased rT3 excretion was found during the infusion of alpha1-agonist methoxamine (1.5 mg/kg/4 h), while no such effect of the infusion of alpha2-agonist azepexol (10 mg/kg/4 h) was observed. It may be suggested that the effect of adrenaline was mediated predominantly by alpha1-adrenoceptors and that the observed changes in biliary excretion of T3 and rT3 were related to the inhibition of 5’-monodeiodination in the liver.
Similar content being viewed by others
References
Wartofsky L., Burman K.D. Alterations in thyroid function in patients with systemic illness: the “Euthyroid sick syndrome”. Endocr. Rev. 3: 164, 1982.
Cavalieri R.R., Pitt-Rivers R. The effects of drugs on the distribution and metabolism of thyroid hormones. Pharmacol. Rev. 33: 55, 1981.
Yamamoto M., Sakurada T., Yoshida K., Kaise K., Kaise N., Fukuzawa H., Suzuki M., Nomura T., Itagaki Y., Saito S., Yoshinaga K. Effect of Carteolol, indenolol and metoprolol on the thyroid hormone of hyperthyroid patients. Tohoku J. Exp. Med. 146: 386, 1985.
Perrild H., Hansen J.M., Skovsted L., Korsgaard Christensen L. Different effects of propranolol, alprenolol, Sotalol, atenolol and metoprolol on serum T3 and serum rT3 in hyperthyroidism. Clin. Endocrinol. (Oxf.) 18: 139, 1983.
Jurney T.H., Smallridge R.C., Routledge P.A., Shand D.G., Wartofsky L. Propranolol decreases serum thyroxine as well as triiodothyronine in rats: a protein-binding effect. Endocrinology 112: 727, 1983.
Heyma P., Larkins R.G., Campbell D.G. Inhibition by propranolol of 3, 5, 3′-triiodothyronine formation from thyroxine in isolated rat renal tubules: an effect independent of α-adrenergic blockade. Endocrinology 106: 1437, 1980.
Wiersinga W.M, Modderman P., Touber J.L. The effect of α- and β-adrenoceptor agonists and antagonists on the in vitro conversion of thyroxine into triiodothyronine. Horm. Metab. Res. 12: 346, 1980.
Ceremuzynski L., Herbaczynska-Cedro K., Broniszowska-Ardelt B., Nauman J., Nauman A., Wozniewicz B., Lawecki J. Evidence for the detrimental effect of adrenaline infused to healthy dogs in doses imitating spontaneous secretion after coronary occlusion. Cardiovasc. Res. 12; 179, 1978.
Nauman A., Kaminski T., Herbaczynska-Cedro K. In vivo and in vitro effects of adrenaline on conversion of thyroxine to triiodothyronine and to reverse-triiodothyronine in dog liver and heart. Eur. J. Clin. Invest. 10: 189, 1980.
Nauman A., Porta S., Bardowska U., Fiedorowicz K., Sadjak A., Korsatko W., Nauman J. The effect of adrenaline pretreatment on the in vitro generation of 3,5,3′-triiodothyronine and 3, 3′, 5′-triiodothyronine in rat liver preparation. Horm. Metab. Res. 16: 471, 1984.
Aanderud S., Aarbakke J., Sundzfjord J. Effect of different α-blocking drugs and adrenaline on the conversion of thyroxine to triiodothyronine in isolated rat hepatocytes. Horm. Metab. Res. 18: 110, 1986.
Keck F.S., Loos V., Wolf Ch.-F., Meyerhoff C., Pfeiffer E.F. Adrenergic modulation of T4-conversion in rat liver microsomes (Abstract). Ann. Endocrinol. (Paris) 47: 24, 1986.
Földes O., Langer P., Straussova K., Brozmanova H., Gschwendtova K. Direct quantitative estimation of several iodothyronines in rat bile by radioimmunoassay and basal data on their biliary excretion. Biochim. Biophys. Acta 716: 383, 1982.
Földes O., Langer P., Brozmanova H., Straussova K., Gschwendtova K. In vivo study of iodothyronine deiodination in rat liver: effect of salicylate on biliary excretion of several iodothyronines. Horm. Metab. Res. 15: 147, 1983.
Langer P., Földes O., Straussova K., Gschwendtova K. Preliminary observations on the absorption of biliary iodothyronines from the intestine in vivo in rats. Endocrinol. Exp. 16: 117, 1982.
Langer P., Suzuki M., Kakegawa T., Földes O., Gschwendtova K. Acute changes of iodothyronine excretion by bile after a single and repeated administration of dexamethasone in rats. Endocrinol. Exp. 19: 253, 1985.
Klaassen C.D., Watkins III J.B. Mechanisms of bile formation, hepatic uptake and biliary excretion. Pharmacol. Rev. 36: 1, 1984.
Kuipers F., Dijkstra T., Havinga R., van Asselt W., Vonk R.J. Acute effects of pentobarbital-anesthesia on bile secretion. Biochem. Pharmacol. 34: 1731, 1985.
Lucas P.D. Effects of stroptozotocin-induced diabetes and noradrenaline infusion on cardiac output and its regional distribution in pithed rats. Diabetologia 28: 108, 1985.
Pang C.C.Y., Tabrizchi R. The effects of noradrenaline, B-HT 920, methoxamine, angiotensin II and vasopressin on mean circulatory filling pressure in conscious rats. Br. J. Pharmacol. 89: 389, 1986.
Silva J.E., Larsen P.R. Hormonal regulation of iodothyronine 5′-deiodinase in rat brown adipose tissue. Am. J. Physiol. 251: E639, 1986.
Obregon M.J., Mills I., Silva J.E., Larsen P.R. Catecholamine stimulation of iodothyronine 5′-deiodinase activity in rat dispersed brown adipocytes. Endocrinology 120: 1069, 1987.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Langer, P., Földes, O. Effect of adrenaline on biliary excretion of triiodothyronines in rats mediated by alpha1-adrenoceptors and related to the inhibition of 5’-monodeiodination in liver. J Endocrinol Invest 11, 471–476 (1988). https://doi.org/10.1007/BF03350162
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF03350162