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Qualitative and quantitative defects of thyroglobulin resulting in congenital goiter. Absence of gross gene deletion of coding sequences in the TG gene structure

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Abstract

Seven subjects belonging to three families (ME, MA, MO), with congenital goiter and various degrees of thyroid hypofunction, were investigated from the standpoints of clinical, biochemical, and molecular biology. In two of these families (ME, MA), 6 individuals had low serum levels of Tg-related antigens with a minor increase after bovine TSH (bTSH) stimulation. A large proportion of the tracer was incorporated into serum albumin, and Tg antigens in the thyroid extracts were barely detectable by RIA. (0.19 mg/g tissue; normal, 70–90 mg/g). Gel filtration (CL6B Sepharose gel) showed absence of a normal Tg peak, and SDS agarose gel electrophoresis indicated complete absence of Tg dimer and monomer. Immunoelectrophoresis confirmed the absence of Tg-related antigens. Thus, in these patients a quantitative defect of Tg gene expression was characterized. By contrast, in the MO family a high basal serum concentration of immunoreactive Tg was present, with an exaggerated response to bTSH. Thyroid extracts revealed elevated TPO activity and normal levels of Tg-related antigens. Tg was also eluted in the gel filtration columns with the same mobility as standard 19S Tg. Immunoelectrophoresis against rabbit and human Tg was abnormal, with two precipitin arcs being detected. The Tg molecule after hydrolysis yielded only DIT and MIT, with poor formation of iodothyronines. Microscopic studies revealed a pronounced lack of colloid in the follicular lumina, and overdistended endoplasmic reticulum cisternae. It was concluded that in this family the defective Tg gene expression is qualitative, with an abnormal Tg being formed and only partially transported to the follicular lumen. Southern blotting experiments to probe Tg gene structure indicated no significant differences in pattern between DNA samples from controls and those from goitrous patients. This suggests defective translation or abnormal routing of translation product through the membrane system of the cell. Further studies of the Tg mRNA and DNA structure will be needed to clarify the exact nature of both defects.

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Authors and Affiliations

  1. Thyroid Laboratory, Division of Endocrinology, Hospital das Clinicas, Univ. São Paulo Med. School, Brazil

    G. Medeiros-Neto, M. Knobel, M. Alkmin, S. Barbosa & B. L. Wajchenberg

  2. Lab. Biol. Molecular, Hospital das Clinicas “José De San Martin”, Fac. Med. Univ. Buenos Aires, Argentina

    H. Targovnik, F. Propato & V. Varela

Authors
  1. G. Medeiros-Neto
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  2. H. Targovnik
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  3. M. Knobel
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  4. F. Propato
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  5. V. Varela
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  6. M. Alkmin
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  7. S. Barbosa
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  8. B. L. Wajchenberg
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Additional information

Supported in part by grants from SANBRA (São Paulo, Brazil), Fundación Bunge & Born, Programa Nacional de Biotecnologia, SECYT, Argentina (326/86), CONICET (19844/85, 9780/86) and Universidad de Buenos Aires (017 ME/87). H. Targovnik is a member of the “Carrera del Investigador”, CONICET, Argentina.

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Medeiros-Neto, G., Targovnik, H., Knobel, M. et al. Qualitative and quantitative defects of thyroglobulin resulting in congenital goiter. Absence of gross gene deletion of coding sequences in the TG gene structure. J Endocrinol Invest 12, 805–813 (1989). https://doi.org/10.1007/BF03350067

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