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Two cases of Graves’ disease with antithyroid hormone antibodies: Implication on the role of thyroglobulin as an antigen

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Abstract

We have experienced two cases of Graves’ disease with antithyroid hormone autoantibodies (Case 1: anti-T4; Case 2: anti-T3) who finally underwent subtotal thyroidectomy after antithyroid drug treatment. Using serial sera obtained before and after operation, the correlation between titers of antithyroglobulin (anti-Tg) and anti-T4 or anti-T3 autoantibodies was examined in each case. There was a significant positive correlation between titers of anti-T4 (Case 1, r = 0.90, p < 0.05), or anti-T3 (Case 2, r = 0.64, p < 0.01) and anti-Tg antibodies. Using the homogenate of the thyroid tissue, it was found that the sole iodoprotein in the thyroid gland in each patient was 660 KDa Tg. In addition, Tg purified from the thyroid gland from Case 2 showed different immunological activity with normal Tg in two out of four murine monoclonal anti-Tg antibodies tested. On the other hand, Tg from Case 1 had identical immunological activity with normal Tg in every four monoclonal antibodies. These results are consistent with the view that the antigen responsible for the development of antithyroid hormone autoantibodies is Tg, at least in our two cases. The reason for the persistence of anti-T3 autoantibodies in Case 2, despite the subtotal thyroidectomy, could be due to some unidentified structural abnormalities of Tg which was detected only by the monoclonal anti-Tg antibodies.

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References

  1. Sakata S., Nakamura S., Miura K. Autoantibodies against thyroid hormone or iodothy- ronines: Implication in diagnosis, thyroid function, treatment, and pathogenesis. Ann. Intern. Med. 103: 579, 1985.

    Article  CAS  PubMed  Google Scholar 

  2. Premachandra B.N., Blumenthal H.T. Significance of thyroid hormone autoantibodies. In: Walfish P.G., Wall J.R., Volpe R. (Eds.), Autoimmunity and the thyroid. Academic Press Inc. Orlando, FL, 1985, p. 190.

    Google Scholar 

  3. Benvenga S., Trimarchi F., Robbins J. Circulating thyroid hormone autoantibodies. J. Endocrinol. Invest. 10: 605, 1987.

    CAS  PubMed  Google Scholar 

  4. Sakata S., Komaki T., Nakamura S., Suzuki S., Torigai K., Kojima M., Miura K. A case of systemic lupus erythematosus (SLE) and Sjogren’s syndrome associated with anti-T3 autoantibodies. Endocrinol. Jpn. 34: 497, 1987.

    Article  CAS  PubMed  Google Scholar 

  5. Pearce C.J., Byfield P.E.G., Edomons C.J., Lalloz M.R.A., Himsworth R.L Autoantibodies to thyroglobulin cross reacting with iodothyronines. Clin. Endocrinol. (Oxf.) 75:1, 1981.

    Article  Google Scholar 

  6. Ochi Y., Shiomi T., Hachiya T, Yoshimura M., Miya zaki T. Immunological analysis of abnormal binding of thyroid hormone in the gamma-globulin. J. Clin. Endocrinol. Metab. 35: 743, 1972.

    Article  CAS  PubMed  Google Scholar 

  7. Byfield P.G.H., Bond A., Copping S., Himsworth R.L. Restricted antigenicity of thyroxine in human thyro globulin. Biochem. J. 207:471, 1982.

    CAS  PubMed Central  PubMed  Google Scholar 

  8. Izumi M., Larsen P.R. Triiodothyronine, thyroxine and iodine in purified thyroglobulin from patients with Graves’ disease. J. Clin. Invest. 59:1105, 1977.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  9. Nilsson L, Rose N.R., Witebsky E. Spontaneous thyroiditis in the obese strain of chickens. VI. Thyroxine-binding antibodies. J. Immunol. 707: 997, 1971.

    Google Scholar 

  10. Brown T.R., Bagchi N., Sundick R.S. Analysis of triiodothyronine and thyroxine-binding autoantibodies in chickens susceptible to autoimmune thyroiditis. J. Immunol. 134:3845, 1985.

    CAS  PubMed  Google Scholar 

  11. Premachandra B.N., Ray A.K., Hirata Y., Blumenthal HT. Electrophoretic studies of thyroxine and triiodothyronine binding by the sera of guinea pigs immunized against thyroglobulin. Endocrinology 73:133, 1963.

    Google Scholar 

  12. Sakata S., Nakamura S., Komaki T., Miura K. Production of anti-human thyroglobulin and anti-thy-roid hormone antibodies in rabbits immunized with human thyroglobulin. Endocrinol. Jpn. 32: 65, 1985.

    Article  CAS  PubMed  Google Scholar 

  13. Sakata S, Okuda K., Aihara Y., Tanaka Y., Nakamura S., Komaki T., Tarutani O., Miura K. Genetic control of the production of anti-thyroid hormone antibodies in mice immunized with human thyroglobulin. Immunol. Invest. 15: 481, 1986.

    Article  CAS  PubMed  Google Scholar 

  14. Sakata S., Nakamura S., Komaki T., Matsuda M., Kojima N., Tarutani O., Miura K. Autoimmune recognition of thyroglobulin and thyroid hormones in rabbits. J. Prot. Chem. 5:407, 1986.

    Article  CAS  Google Scholar 

  15. Nakamura S., Sakata S., Shima H, Komaki T., Kojima N., Kamikubo K., Yasuda K., Miura K. Thyroid hormone autoantibodies (THAA) in two cases of Graves’ disease. Effect of antithyroid drugs, prednisolone, and subtotal thyroidectomy. Endocrinol. Jpn. 33: 751, 1986.

    Article  CAS  PubMed  Google Scholar 

  16. Kohno Y., Tarutani O, Sakata S., Nakashima Monoclonal antibodies to thyroglobulin elucidate differences in protein structure of thyroglobulin in healthy individuals and those with papillary adenocarcinoma. J. Clin. Endocrinol. Metab. 61: 343, 1985.

    Article  CAS  PubMed  Google Scholar 

  17. Hunter W.M., Greenwood F.C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature 794:495, 1962.

    Article  Google Scholar 

  18. Robbins Rail J.E., Rawson R.W. An unusual instance of thyroxine binding by human serum gamma globulin. J. Clin. Endocrinol. Metab. 16: 573, 1956.

    Article  CAS  PubMed  Google Scholar 

  19. Sakata S. On the role of thyroglobulin as an antigen of thyroid hormone autoantibodies. Clin. Chim. Acta 1990 in press.

    Google Scholar 

  20. Mercken L, Simons Swillens Massaer M., Vassart G. Primary structure of bovine thyroglobulin deduced from sequence of its 8, 431-base complementary DNA. Nature 316: 647, 1985.

    Article  CAS  PubMed  Google Scholar 

  21. Malthiery Y., Lissitzky S. Primary structure of human thyroglobulin from sequence of its 8448-base complementary DNA. Eur. J. Biochem. 165: 491, 1987.

    Article  CAS  PubMed  Google Scholar 

  22. Robbins J., Salvatore G., Vecchio G., Ui N. Thyroglobulin and 27-S iodoprotein. lodination and ultracentrifugai heterogeneity. Biochim. Biophys. Acta 127:101, 1966.

    Article  CAS  PubMed  Google Scholar 

  23. Thomson J.A., Goldberg I.H. Biosynthesis of thyroglobulin and its subunit in the rat thyroid gland. Endocrinology 82: 805, 1968.

    Article  CAS  Google Scholar 

  24. Kimata Y., Sakata S., Kojima N., Horiya Y., Tarao M., Komaki T., Miura K. Anti-thyroglobulin autoantibodies in patients with benign and malignant thyroid tumors. In: Lee M., Koh C.S., Eastman C.J., Nagataki S., (Eds.), Progress in thyroidology 1989. Korea Medical Publishing Co, Seoul, 1989, p. 499.

    Google Scholar 

  25. Sakata S., Nakamura S., Komaki T., Kojima N., Takuno H., Miura K., Kamikubo K. Theoretical consideration of antigenicity of hormo-genic tyrosines in human thyroglobulin. Folia. Endocrinol. Jpn. 64: 941, 1988. (Abstract)

    Google Scholar 

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Authors and Affiliations

  1. The Third Department of Internal Medicine, Gifu University School of Medicine, Gifu, 500, Japan

    S. Sakata, T. Komaki, H. Takuno, M. Matsuda, T. Ogawa & K. Miura

  2. Department of Internal Medicine, Takayama Red Cross Hospital, Takayama, 506, Japan

    K. Nagai & N. Tokimitsu

  3. Division of Biological Chemistry, Science University of Tokyo, Noda, 278, Japan

    O. Tarutani

  4. Department of Pediatrics, Chiba University School of Medicine, Chiba, 280, Japan

    Y. Kohno & K. Saito

Authors
  1. S. Sakata
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  2. K. Nagai
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  3. O. Tarutani
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  4. Y. Kohno
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  5. K. Saito
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  6. T. Komaki
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  7. H. Takuno
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  8. M. Matsuda
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  9. T. Ogawa
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  10. N. Tokimitsu
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  11. K. Miura
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Sakata, S., Nagai, K., Tarutani, O. et al. Two cases of Graves’ disease with antithyroid hormone antibodies: Implication on the role of thyroglobulin as an antigen. J Endocrinol Invest 13, 825–832 (1990). https://doi.org/10.1007/BF03349632

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  • DOI: https://doi.org/10.1007/BF03349632

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