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Inhibitory effect of the somatostatin analog octreotide on rat pituitary tumor cell (GH3) proliferation in vitro

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Abstract

The effects of somatostatin-14 (SS-14) and the somatostatin-analog octreotide (SMS 201-995, Sandostatin) on proliferation of GH3 pituitary tumor cells were investigated in vitro. SMS 201-995 exerted a significant, but transient, inhibition on GH3 cell growth which reached a maximum at 24 h and was no longer detectable at 48 h. The concentration that evoked the strongest inhibitory effect was 10 nM SMS 201-995, while lower and higher doses resulted in a less pronounced effect. The inhibitory effect SMS 201-995 exerted on cell proliferation was associated with a dose- and time-related reduction in both c-myc and c-fos mRNA levels. SS-14 had no noteworthy influence on either cell proliferation or c-myc and c-fos protooncogene expression. These data demonstrate that SS-analogs transiently inhibit pituitary tumor cell proliferation in vitro.

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  1. Istituto di Clinica Medica I, Università di Perugia, Policlinico Monteluce, I-06100, Perugia, Italy

    G. Pelicci, M. C. Pagliacci, L. Lanfrancone, P. G. Pelicci, F. Grignani & Ildo Nicoletti M.D.

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  1. G. Pelicci
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  2. M. C. Pagliacci
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  3. L. Lanfrancone
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  4. P. G. Pelicci
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  5. F. Grignani
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  6. Ildo Nicoletti M.D.
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Pelicci, G., Pagliacci, M.C., Lanfrancone, L. et al. Inhibitory effect of the somatostatin analog octreotide on rat pituitary tumor cell (GH3) proliferation in vitro . J Endocrinol Invest 13, 657–662 (1990). https://doi.org/10.1007/BF03349589

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  • DOI: https://doi.org/10.1007/BF03349589

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