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Effect of amiodarone on L-triiodothyronine stimulation of [3H] thymidine incorporation into GH3 cells

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Abstract

The antiarrhythmic agent amiodarone was found to inhibit the stimulatory effects of L-triiodothyronine on [3H ] thymidine incorporation into GH3 rat pituitary tumor cells. This inhibitory effect of amiodarone was detected at concentrations as low as 0.5 μM; at 2 μM greater than 50% of the stimulatory effect of L-triiodothyronine was inhibited. The effect of amiodarone was present at all concentrations of L-triiodothyronine tested (50 pM to 10 nM), suggesting that amiodarone acted as a non-competitive antagonist. These studies raise the possibility, therefore, that the effect of amiodarone on thyroid hormone metabolism may be mediated in part by an inhibition of thyroid hormone action at the cellular level.

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Authors and Affiliations

  1. Cell Biology Laboratory and Department of Medicine, Mount Zion Hospital and Medical Center, P.O.Box 7921, San Francisco, CA, 94120, USA

    I. D. Goldfine, B. Maddux & K. A. Woeber

  2. Department of Medicine, University of California, San Francisco, CA, 94143, USA

    I. D. Goldfine & K. A. Woeber

  3. Department of Physiology, University of California, San Francisco, CA, 94143, USA

    I. D. Goldfine

Authors
  1. I. D. Goldfine
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  2. B. Maddux
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  3. K. A. Woeber
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Goldfine, I.D., Maddux, B. & Woeber, K.A. Effect of amiodarone on L-triiodothyronine stimulation of [3H] thymidine incorporation into GH3 cells. J Endocrinol Invest 5, 165–168 (1982). https://doi.org/10.1007/BF03349473

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  • DOI: https://doi.org/10.1007/BF03349473

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