Abstract
Although the role of biogenic amines in the regulation of gonadotropin release has been studied extensively, the precise role of dopamine (DA) in stimulating LHRH and/or LH release is still controversial. In the present study 6 eugonadal women, aged 20–30, were given an LHRH infusion on day 6 of the menstrual cycle and the pattern and magnitude of LH and FSH responses were estimated. On day 6 of the next cycle, the experiment was repeated and DA was also infused beginning 60 min after the start of the LHRH infusion. Following LHRH infusion plasma LH showed a marked and significant rise with a mean peak increment at 4 h, and with a cumulative response (CR) of 9136 ± 955 mlU/ml/6h. The pattern of FSH response tended to parallel that of LH; however, mean peak increment at 4 h and a CR of 2640±169 mlU/ml/6h were markedly lower. Plasma prolactin levels remained unchanged. Addition of DA to LHRH at 60 min evoked a significantly greater mean peakLH increment at 4 h and a CR of 15514±1836 mlU/ml/6h (p < 0.001). There were no significant changes in either mean FSH peak at 4h or in the CR (3257 ± 309 mlU/ml/6h). As expected, a highly significant (p < 0.001) decrease in circulating PRL from 12.1 ± 3.1 to 3.0 ± 1.5 ng/ml was seen during DA infusion. In conclusion, DA given by iv infusion enhances LH response to LHRH in eugonadal women under the conditions of the present investigation, supporting a role for a dopaminergic component in the control of LH release in women.
Similar content being viewed by others
References
Schneider H.P.C., McCann S.M. Release of LH releasing factor (LRF) into the peripheral circulation of hypophysectomized rats by dopamine and its blockage by estradiol. Endocrinology 87: 249, 1970.
Kamberi I.A., Mical R.S., Porter J.C. Effect of anterior pituitary perfusion and intraventricular injection of catecholamines and indoleamines on LH release. Endocrinology 87: 1, 1970.
McCann S.M., Klara P.S., Donoso A.O., Bishop W., Schneider H.P.C., Fawcett C.P., Krulich W. The role of monoamines in the control of gonadotrophin and prolactin secretion. In: Knigge K.M., Scott D.E., Weinol A., (Eds.), Brain endo-crine interaction. Median eminence: structure and function. Karger, Basel, 1972.
Rotsezejn S., Martin M., Grymm Y. Demonstration of LRF, FRL and IF in pituitary stalk. Recent Prog. Horm. Res. 28: 229, 1972.
Delitala G., Masala A., Rolandi E. Effect of L-dopa on plasma HGH, LH, FSH and 11-OH-CS in normal adult subjects. Folia Endocrinol. 27: 279, 1974.
Leblanc H., Lachelin G.C.L., Abu-Fadil S., Yen S.S.C. Effects of dopamine infusion on pituitary hormone secretion in humans. J. Clin. Enodcrinol. Metab. 43: 668, 1976.
Fuxe K., Loftrom A., Eneroth P., Gustafsson J.A., Hokfelt T., Scett P., Wuttke W., Fraser H., Jeffcoate S. Interaction between hypothalamic catecholamine nerve terminals and LRF containing neurons. Further evidence for an inhibitory dopaminergic and a facilitory noradrenergic influence. In: Charro Salgado A.L., Fernandez-Durango A., Lopez del Campo J.C. (Eds.), Basic application and clinical uses of hypothalamic hormones. Excerpta Medica, Amsterdam, p. 165, 1976.
Wide L., Nillius S.J., Gemzel C., Roos P. Radioimmunosorbent assay of FSH and LH in serum and urine from men and women. Acta Endocrinol. (Kbh.) (Suppl. 174): 7, 1973.
Franchimont P., Demoulin A., Bourguignon J.P. Clinical use of LH-RH test as a diagnostic tool. Horm. Res. 6: 177, 1975.
Ciardella F., Galli P., Sgrilli R., Franchi F., Catarsi A.L., Birindelli R., Luisi M. Radioimmunoassay for plasma prolactin in humans. Bollettino della Societa di Medicina Chirurgica (Pisa) 44: 219, 1976.
Snedecor G.W., Cochran W.G. Statistical methods. The Iowa University Press, 1969.
Pucci E., Montanelli G., Bartolini E., Bernini G.P., Volpe L., Franchi F., Luisi M. Studio della riserva gonadotropinica tramite la somministrazione di LHRH In: Tronchetti F., Menchini-Fabris G.F. (Eds.), Atti delle Giornate Endocrinologiche Pisane Pacini Ed., Pisa, 1977, p. 277.
Hoff J.D., Lasley B.L., Wang C.F., Yen S.S.C. The two pools of pituitary gonadotropin: regulation during the menstrual cycle. J. Clin. Endocrinol. Metab. 44: 302, 1977.
Konincsky P., DeHertoghe R., Heyns W., Meulepas E., Brosens I., De Moor P. Secretion rates of LH and FSH during infusion of LH/FSH-RH in normal women and in patients with secondary amenorrhea: suggestive evidence of two pools of LH and FSH. J. Clin. Endocrinol. Metab. 43: 159, 1976.
Souvatzoglou A., Bottermann P., Werder K.V., Schwarz K. Effect of L-dopa on LH and GH levels in man. Acta Endocrinol. (Kbh.), (Suppl. 159): 6, 1972.
Smythe G.A. The role of serotonin and dopamine in hypothalamic-pituitary function. Clin. Endocrinol. (CM), 7: 325, 1977.
Judd S.J., Rakoff J.S., Yen. S.S.C. Inhibition of gonadotropin and prolactin release by dopamine: effect of endogenous estradiol levels. J. Clin. Endocrinol. Metab. 47: 494, 1978.
Judd S.J., Rigg L.A., Yen S.S.C. The effects of ovariectomy and estrogen treatment on the dopamine inhibition of gonadotropin and prolactin release. J. Clin. Endocrinol. Metab, 49: 182, 1979.
Diefenbach C.H. Suppression of prolactin secretion by L-dopa in stalk-sectioned monkeys. J. Clin. Endocrinol. Metab. 43: 638, 1976.
Schneider H.P.G., McCann S.M. Possible role of dopamine as transmitter to promote discharge of LH-releasing factor. Endocrinology 85: 121, 1969.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pucci, E., Franchi, F., Kicovic, P.M. et al. Amplification of LH response to LHRH by dopamine infusion in eugonadal women. J Endocrinol Invest 4, 55–58 (1981). https://doi.org/10.1007/BF03349415
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF03349415