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The effect of sulphydryl reagents on the human thyroid microsomal antigen

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Abstract

High sensitivity of the microsomal antigen epitopes recognized by autoantibodies in sera of patients with thyroid autoimmune diseases to disulphide bridge reducing agents have been detected. The shape of the inactivation curve is different for sera obtained from different patients, but for most sera 50% inactivation is obtained below 1 mM of dithioerythritol. The inactivation strongly depends on the pH of the incubation buffer and is most pronounced at alkaline pH (9.6). Cross-linking of the soluble microsomal antigen by glutaraldehyde makes the epitopes resistant to DTE. Binding of the microsomal antigen to polystyrene makes it up to 5 times more resistant to DTE. The microsomal peroxidase enzymatic activity is destroyed by low concentration of dithioerythritol and the inactivation is not dependent on the buffer pH (7.4–9.6). The data demonstrated that the major microsomal antigen epitope is discontinuous (conformation dependent).

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References

  1. Bottazzo G.F., Drexhange H.A., Khoury E.L. Thyroid antibodies in thyroid diseases. In: Receptors antibodies and diseses. London Pitman, Ciba Foundation Symposium, no. 153, 1982.

  2. Khoury E.L., Bottazzo G.F., Roitt I.M. The thyroid microsomal antibody revisited: its paradoxical binding in vivo to the apical surface of the follicular epithelium. J. Exp. Med. 159: 577, 1984.

    Article  CAS  PubMed  Google Scholar 

  3. Pinchera A., Fenzi G.F., Bartalena L., Chiovato L., Marcocci C., Baschieri L. Antigen-antibody system involved in thyroid autoimmunity. In: Pinchera A., Doniach D., Fenzi G.F., Baschieri L. (Eds.), Autoimmune aspects of endocrine disorders. Academic Press, London, 1980, p. 57.

    Google Scholar 

  4. Czarnocka B., Ruf J., Ferrand M., Carayon P., Lissitzky S. Purification of the human thyroid peroxidase and its identification as the microsomal antigen involved in autoimmune thyroid diseases. FEBS Lett. 190: 147, 1985.

    Article  CAS  PubMed  Google Scholar 

  5. Kotani T., Umeki K., Matsunaga S., Kato E., Ohtaki S. Detection of autoantibodies to thyroid peroxidase in autoimmune thyroid diseases by micro-ELISA and immunoblotting. J. Clin. Endocrinol. Metab. 62: 928, 1986.

    Article  CAS  PubMed  Google Scholar 

  6. Garcia-Amaijeras A., Duque A., Rossell E., Corcoy R. Identification of different immunoglobulin G epitopes in Graves’ disease. In: Drexhage H.A., Wiersinga W.M. (Eds), The thyroid and autoimmunity. Excerpta Medica, Amsterdam, 1986, p. 19.

    Google Scholar 

  7. Islam M.N., Briones-Urbina R., Bako G., Farid N.R. Both TSH and thyroid-stimulating antibody of Graves’ disease bind to an Mr 197, 000 holoreceptor. Endocrinology 113: 436, 1983.

    Article  CAS  PubMed  Google Scholar 

  8. Hamada N., Jaeduck N., Portman L., Kunihiko I., De-Groot L.J. Antibodies against denatured and reduced thyroid microsomal antigen in autoimmune thyroid disease. J. Clin. Endocrinol. Metab. 64: 230, 1987.

    Article  CAS  PubMed  Google Scholar 

  9. Nakajima Y., Howells R.D., Pegg C., Jones E.D., Smith B.R. Structure-activity analysis of microsomal antigen/thyroid peroxidase. Mol. Cell. Endocrinol. 53: 15, 1987.

    Article  CAS  PubMed  Google Scholar 

  10. Mariotti S., Russova A., Pisani S., Pinchera A. A new solid phase immunoradiometric assay for antithyroid microsomal antibody. J. Clin. Endocrinol. Metab. 56: 467, 1983.

    Article  CAS  PubMed  Google Scholar 

  11. Gardas A., Rives K.L. Enzyme linked immunosorbent assay of autoantibodies reacting with thyroid plasma membrane antigens in sera of patients with autoimmune thyroid diseases. Acta Endocrinol. (Kbh.) 113: 255, 1986.

    CAS  Google Scholar 

  12. Schardt C.W., McLanchlan S.M., Matheson J., Smith B.R. An enzyme-linked immunoassay forthyroid microsomal antibodies. J. Immunol. Methods 55: 155, 1982.

    Article  CAS  PubMed  Google Scholar 

  13. Benjamin D.C., Berzofsky J.A., East I.L., Gurd F.R.N., Hannum C., Leach S.J., Margoliash E., Michael J.G., Miler A., Prager E.M., Reichlin M., Sercarz E.E., Smith-Gill S.J., Todd P., Wilson A. The antigenic structure of proteins: a reappraisal. Ann. Rev. Immunol. 2: 67, 1984.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Medical Center of Postgraduate Education, Biochemistry Department, Marymoncka 99, 01 -813, Warsaw, Poland

    A. Gardas M.D. & H. Domek

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  1. A. Gardas M.D.
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  2. H. Domek
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The work was supported by the grant MZX-1 from Polish Ministry of Health.

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Gardas, A., Domek, H. The effect of sulphydryl reagents on the human thyroid microsomal antigen. J Endocrinol Invest 11, 385–388 (1988). https://doi.org/10.1007/BF03349061

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  • DOI: https://doi.org/10.1007/BF03349061

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