Abstract
Thyroid tissue total RNAs from multinodular goiter(G2) and from hereditary goiter with defective Tg synthesis (JNA) were hybridized with a 5’albumin cDNA probe (F-47), a 3’ albumin cDNA probe (B-44) and a thyroglobulin cDNA probe (phTgM3). JNA refers to tissue obtained from a patient with virtual absence of Tg in thyroid tissue and the presence of increased concentration of an albumin-like labeled protein in the thyroid. No hybridization signal was detected in both G2 and JNA with albumin probes at Northern Blot studies. Those results were confirmed by dot-blot analysis of total RNA where no hybridization signal was detected in G2 and JNA. To confirm that thyroid tissues do not express thyroalbumin total RNA from JNA and normal control thyroid tissue (C) were amplified by PCR using albumin and Tg primers. An expected fragment of 592 bp was observed in a human liver sample with the albumin primers. However JNA and C samples showed absence of an amplification product of the same size. We concluded that thyroid cells do not contain the albumin transcript. Albumin is probably taken up from circulation and iodinated by the thyroid follicular cell with subsequent release of iodoalbumin into the circulation.
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Abbreviations
- PCR:
-
polymerase chain reaction
- nt:
-
nucleotide
- bp:
-
base pairs
- dNTP:
-
deoxyribonucleotide triphosphate
References
Christophe D., Vassart G. The thyroglobulin gene. Evolutionary and regulatory issues. Trends Endocrinol. Metab. 1: 351, 1990.
Lissitzky S., Codaccioni J.L., Cartouzou G., Mante S. Eumetabolic goitrous adult with iodoprealbumin in thyroid tissue and blood. J. Clin. Endocrinol. Metab. 24: 305, 1964.
Lissitzky S., Bismuth J., Codaccioni J.L., Cartouzou G. Congenital goiter with iodoalbumin replaeing thyroglobulin and defect of deiodination of iodotyrosines. Serum origin of the thyroid iodoalbumin. J. Clin. Endocrinol. Metab. 28: 1797, 1968.
Furth E.D., Agrawal R.B., Propp R.P. Secretion of iodoalbumin and iodoprealbumin by a congenital goiter containing thyroglobulin and the iodoalbumins. J. Clin. Endocrinol. Metab. 31: 60, 1970.
Lissitzky S., Bismuth J., Jaquet P., Castay M., Michel-Bechet M., Koutras D.A., Pharmakiotis A.D., Moschos A., Psarras A., Malamos B. Congenital goiter with impaired thyroglobulin synthesis. J. Clin. Endocrinol. Metab. 36: 17, 1973.
Desai K.B., Mehta M.N., Patel M.C., Sharma S.M., Ramanna L., Ganatra R.D. Familial goitre with absence of thyroglobulin and synthesis of thyroid hormones from thyroidal albumin. J. Endocrinol. 60: 389, 1974.
Cabrer B., Brocas H., Perez Castillo A., Pohl V., Navas J.J., Targovnik H., Centenera J.A., Vassart G. Normal level of thyroglobulin messenger ribonucleic acid and human congenital goiter with thyroglobulin deficiency. J. Clin. Endocrinol. Metab. 63: 931, 1986.
Targovnik H., Propato F., Varela V., Wajchenberg B., Knobel M., D’Ambronzo H.F., Medeiros-Neto G. Low levels of thyroglobulin messenger ribonucleic acid in congenital goitrous hypothyroidism with defective thyroglobulin synthesis. J. Clin. Endocrinol. Metab. 69: 1137, 1989.
Targovnik H.M., Varela V., Juvenal G.J., Propato F., Chester H.A., Krawiec L., Frechtel G., Moran D.H., Perinette H.A., Pisarev M.A. Differential levels of thyroid peroxidase and thyroglobulin messenger ribonucleic acids in congenital goiter with defective thyroglobulin synthesis. J. Endocrinol. Invest. 13: 797, 1990.
Lever E.G., Medeiros-Neto G.A., DeGroot L.J. Inherited disorders of thyroid metabolism. Endocr. Rev. 4: 213, 1983.
Otten J., Jonckheer M.H., Dumont J.E. Thyroid albumin II. In vitro synthesis of a thyroid albumin by normal human thyroid tissue. J. Clin. Endocrinol. Metab. 32: 18, 1971.
Targovnik H.M., Medeiros-Neto G., Varela V., Cochaux P., Wajchenberg B.L., Vassart G. A nonsense mutation causes human hereditary congenital goiter with preferential production of a 171 nt deleted thyroglobulin RNA messenger. J. Clin. Endocrinol. Metab. 1993, in press.
Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinum thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162: 156, 1987.
Fourney R.M., Miyakoshi J., Day III R.S., Paterson M.C. Northern Blotting: Efficient RNA staining and transfer. Focus. 10: 5, 1988.
Lawn R.M., Adelman J., Bock S.C., Franke A.F., Houck C.M., Najarian R.C., Seeburg P.H., Wion K.L. The sequence of human serum albumin cDNA and its expression in E. coli. Nucl. Ac. Res. 9: 6103, 1981.
Bergé-Lefranc J.L., Cartouzou G., Malthiery Y., Perrin F., Jarry B., Lissitzky S. Cloning of four DNA fragments complementary to human thyroglobulin messenger RNA. Eur. J. Biochem. 120: 1, 1981.
Feinberg A.P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity (Addendum). Anal. Biochem. 137: 266, 1984.
White B.A., Bancroft F.C. Cytoplasmic dot hybridization. Simple analysis of relative mRNA levels in multiple small cell or tissue samples. J. Biol. Chem. 257: 8569, 1982.
Lamas L., Dorris M.L., Taurog A. Evidence for a catalytic role of thyroid peroxidase in the conversion of deiodotyrosine to thyroxine. Endocrinology 90: 1417, 1972.
de Vijlder J.J.M., Veenboer G.J.M., van Dijk J.E. Thyroid albumin originates from blood. Endocrinology 131: 578, 1992.
van Dijk J.E. Congenital goitre in Dutch goats: a pathological characterization and an investigation of the origin of abnormal iodoproteins. Doctoral Thesis, University of Utrecht, Elinkwijk BV Printers, Utrecht, pp. 107–126, 1981.
Smeds S. A microgel electrophoretic analysis of the colloid proteins in single rat thyroid follicles. II. The protein concentration of the colloid in single rat thyroid follicles. Endocrinology 91: 1300, 1972.
Pretell E.A., DeGroot L.J., Vickery A.L. Albumin penetration into the rat thyroid gland. Endocrinology. 82: 1103, 1968.
Herzog V. Pathways of endocytosis in thyroid follicle cells. Int. Rev. Cytol. 91: 107, 1984.
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Targovnik, H.M., Frechtel, G.D., Varela, V. et al. Human thyroid tissue do not express thyroalbumin. J Endocrinol Invest 16, 415–419 (1993). https://doi.org/10.1007/BF03348869
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DOI: https://doi.org/10.1007/BF03348869