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Epidermal growth factor receptor in human brain tumors

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Abstract

The expression of epidermal growth factor receptor (EGF-R) was examined in 27 primary human brain tumors (7 glioblastomas, 10 astrocytomas, 5 oligodendrogliomas, 1 schwannoma, 1 ganglioneuroma, 1 medulloblastoma, 1 ependimoma, 1 histiocitic lymphoma), in 6 brain metastases from lung carcinomas and in 20 meningiomas. Peritumoral tissues histologically normal excised surgically along with a large tumor were used as control. All plasma membranes from brain tissues tested showed specific EGF binding. The EGF receptor is expressed at low levels in the control human brain and at very high levels in 60% of the total intracranial tumors studied. When the various histological types of tumors were analyzed, the higher percentage of positive tumors was found with the meningiomas (85%) and the glioblastomas (71%), while the lower percentage of positivity was found with the oligodendrogliomas (40%) and the astrocytomas (30%). A good correlation between binding and total amount of EGF-R protein detected by Western Blot was also observed.

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References

  1. Carpenter G. Receptors for epidermal growth factor and other polypeptide mitogens. Annu. Rev. Biochem. 56:881, 1987.

    Article  PubMed  CAS  Google Scholar 

  2. Downward J., Yarden Y., Mayes E., Scrace G., Totty N., Stockwell P., Ullrich A., Schlessinger J., Waterfield M.D. Close similarity of epidermal growth factor receptor and v-erbB oncogene protein sequences. Nature 307: 521, 1984.

    Article  PubMed  CAS  Google Scholar 

  3. Velu T.J., Beguinot L., Wass W.C., Willingham M.C., Merlino G.T., Pastan I., Lowy D.R. Epidermal growth factor dependent transformation by a human EGF receptor proto-oncogene. Science 238:1408, 1987.

    Article  PubMed  CAS  Google Scholar 

  4. Velu T.J., Beguinot L., Wass W.C., Zhang K., Pastan I., Lowy D.R. Retrovirus expressing different levels of the normal epidermal growth factor receptor: biological properties and new biossay. J. Cell. Biochem. 39:153, 1989.

    Article  PubMed  CAS  Google Scholar 

  5. Di Fiore P.P., Pierce J.H., Fleming T.P., Hazan R., Ullrich A., King C.R., Schlessinger J., Aaronson S.A. Overexpression of the human EGF receptors confers an EGF-dependent transformed phenotype to NIH 3T3 cells. Cell 57:1063, 1987.

    Article  Google Scholar 

  6. Hendler F., Ozanne B. Human squamous cell lung cancers express increased epidermal growth factors receptors. J. Clin. Invest. 74:647, 1984.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  7. Ozanne B., Shum A., Richards C.S., Cassells D., Grossman D., Trent J., Gusterson B., Hendler F. Evidence for an increase of EGF receptors in epidermoid malignancies. In: Cancer Cells, Growth Factors and Transformatio Cold Spring Harbor, NY, 1985, vol. III, p. 41.

    Google Scholar 

  8. Sainsbury J.R.C., Farndom J.R., Needham G.K., Malcolm A.J., Harris A.L. Epidermal growth factor receptor receptor status as predictor of early recurrence of and death from breast cancer. Lancet 7:1398, 1987.

    Google Scholar 

  9. Neal D.E., Marsh C., Bennett M.K., Abel P.D., Hall R.R., Sainsbury J.R.C., Harris A.L. Epidermal growth factor receptors in human bladder cancer: comparison of invasive and superficial tumors. Lancet 7:366, 1985.

    Article  Google Scholar 

  10. Libermann T.A., Razon N., Bartal A.D., Yarden Y., Schlessinger J., Soreq H. Expression of epidermal growth factors receptors in human brain tumors. Cancer Res. 44:753, 1984.

    PubMed  CAS  Google Scholar 

  11. Libermann T.A., Nusbaum H.R., Razon N., Kris R., Lax I., Soreq H., Whittle N., Waterfield M.D., Ullrich A., Schlessinger J. Amplification, enhanced expression and possible rearrangement of EGF receptor gene in primary human brain tumours of glial origin. Nature 313: 144, 1985.

    Article  PubMed  CAS  Google Scholar 

  12. Wong A.J., Bigner S.H., Bigner D.D., Kinzler K.W., Hamilton S.R., Vogelstein B. Increased expression of the epidermal growth factor receptor gene in malignant gliomas is invariably associated with gene amplification. Proc. Natl. Acad. Sci. USA 84: 6899, 1987.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  13. Filmus J., Pollak M.N., Cairncross J.G., Buick R.N. Amplified, overexpressed and rearranged epidermal growth factor receptor gene in a human astrocytoma cell line. Biochem. Biophys. Res. Commun. 131:207, 1985.

    Article  CAS  Google Scholar 

  14. Weisman A.S., Raguet S.S., Kelly P.A. Characterization of the epidermal growth factor receptor in human meningioma. Cancer Res. 47:2172, 1987.

    PubMed  CAS  Google Scholar 

  15. Yamazaki H., Fukui Y., Ueyama Y., Tamaoki N., Kawamoto T., Taniguchi S., Shibuya M. Amplification of the structurally and functionally altered epidermal growth factor receptor gene (c-erbB) in human brain tumors. Mol. Cell. Biol. 8:1816, 1988.

    PubMed Central  PubMed  CAS  Google Scholar 

  16. Zulch K.J. Principles of the new World Health Organization (WHO) classification of the brain tumors. Neuroradiology 79:59, 1980.

    Article  Google Scholar 

  17. Bradford M.M. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248, 1976.

    Article  PubMed  CAS  Google Scholar 

  18. Beguinot L., Werth D., Ito S., Richert N., Willingham M.C., Pastan I. Functional studies on the EGF receptor with an antibody that recognized the intracellular portion of the receptor. J. Biol. Chem. 267:1801, 1986.

    Google Scholar 

  19. Nicholson S., Sainsbury J.R.C., Needham G.K., Chambers P., Farndom J.R., Harris A.L. Quantitative assays of epidermal growth factor receptor in human breast cancer: cut-off points of clinical relevance. Int. J. Cancer 42: 36, 1988.

    Article  PubMed  CAS  Google Scholar 

  20. Slamon D.J., Clark G.M., Wong S.G., Levin W.J., Ullrich A., McGuire W.L. Human breast cancer: correlation of relapse and survival with amplification of the HER-2/neu oncogene. Science 235:177, 1987.

    Article  PubMed  CAS  Google Scholar 

  21. Kamata N., Chida K., Rikimaru K., Horikoshi M., Enomoto S., Kuroki T. Growth-inhibitory effects of epidermal growth factor and over expression of its receptors on human squamous cell carcinomas in culture. Cancer Res. 46:1648, 1986.

    PubMed  CAS  Google Scholar 

  22. Yao M., Shuin T., Misaki H., Kubota Y. Enhanced expression of c-myc and epidermal growth factor receptor (C-erbB-1) genes in primary human renal cancer. Cancer Res. 48:6753, 1988.

    PubMed  CAS  Google Scholar 

  23. Yamamoto T., Kamata N., Kawano H., Shimizu S., Kuroki T., Toyoshima K., Rikimaru K., Nomura N., Ishizaki R., Pastan I., Gamou S., Shimizu N. High incidence of amplification of the epidermal growth factor receptor gene in human squamous carcinoma cell lines. Cancer Res. 46:414, 1986.

    PubMed  CAS  Google Scholar 

  24. Humphrey P.A., Wong A.J., Vogelstein B., Zalutsky M.R., Fuller G.N., Archer G.E., Friedman H.S., Kwatra M.M., Bigner S.H., Bigner D.D. Anti-synthetic peptide antibody reacting at the fusion junction of deletion-mutant epidermal growth factor receptors gene in human glioblastoma. Proc. Natl. Acad. Sci. USA 87:4207, 1990.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  25. Sugawa N., Ekstrand A.J., James C.D., Collins V.P. Identical splicing of aberrant EGF-R transcripts from amplified rearranged genes in human glioblastomas. Proc. Natl. Acad. Sci. USA 87: 8602, 1990.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  26. Derynck R., Goeddel D.V., Ullrich A., Gutterman J.U., Williams R.D., Bringman T.S., Berger W.H. Synthesis of messenger RNAs for transforming growth factors alfa and beta and the epidermal growth factor receptor by human tumors. Cancer Res. 47:707, 1987.

    PubMed  CAS  Google Scholar 

  27. Elia S., Cecere C., Ferrante G., Di Carlo A., Mariano A., Macchia V. Epidermal growth factor receptor (EGFr) in human endothoracic neoplasms. Chest 96:131S, 1989.

    Google Scholar 

  28. Veale D., Kerr N., Gibson G.J., Harris A.L. Characterization of epidermal growth factor receptor in primary human non-small cell lung cancer. Cancer Res. 49:1313, 1989.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Centro di Endocrinologia ed Oncologia Sperimentale del CNR e Dipartimento di Biologia e Patologia Cellulare e Molecolare, II Facoltà di Medicina e Chirurgia, Università di Napoli, Via Sergio Pansini 5, 80131, Napoli, Italy

    A. Di Carlo, A. Mariano, P. E. Macchia &  Vincenzo Macchia

  2. Institute of Microbiology, University of Copenhagen, Denmark

    M. C. Moroni & L. Beguinot

Authors
  1. A. Di Carlo
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  2. A. Mariano
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  3. P. E. Macchia
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  4. M. C. Moroni
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  5. L. Beguinot
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  6. Vincenzo Macchia
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Di Carlo, A., Mariano, A., Macchia, P.E. et al. Epidermal growth factor receptor in human brain tumors. J Endocrinol Invest 15, 31–37 (1992). https://doi.org/10.1007/BF03348650

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