Abstract
Human erythrocyte membrane has been demonstrate to possess an insulin-degrading activity. This activity is not due to a contamination by cytosolic factors and seems to be specific toward insulin. The fractionation of the erythrocyte membrane by Triton X-100 leads to the appraisal of an insulin-degrading activity in the Triton-extracted membranes higher than that present in the solubilized protein fraction. The degrading activity found in the extracted membranes is inhibited by the addition of the solubilized material. This last fraction seems to modulate, in the intact membrane, the whole insulin-degrading system.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Terris S., Steiner D.F. Binding and degradation of 125I-insulin by rat hepatocytes. J. Biol. Chem. 250:8389,1975.
Gliemann J., Sonne O. Binding and receptor-mediated degradation of insulin in adipocytes. J. Biol. Chem. 253:7857,1978.
Herber C.T., Solomon S.S., Duckworth W.C. Time course of insulin-degradation in perfused isolated rat adipose cells. J. Clin. Invest. 65:461,1980.
Varandani P.T. Insulin degradation. X Unmasking of glutathione-insulin transhydrogenase in rat liver microsomal membranes. Biochim. Biophys. Acta 304: 642,1973.
Yokono K., Imamura Y., Sakay H., Baba S. I nsulin degrading activity of plasma membranes from rat skeletal muscle Diabetes 28: 810,1979.
Duckworth W.C. Insulin and glucagon degradation by the kidney. II. Characterization of the mechanism at neutral pH. Biochem. Biophys. Acta 437: 518,1976.
Burghen G.A., Kitabchi A.E., Brush J.S. Characterization of a rat liver protease with specificity for insulin. Endocrinology 91: 633,1972.
Brush J.S. Studies on inhibitors of the insulin protease of rat liver. Biochem. Farmacol. 26: 2349,1977.
Freychet P., Kahn C.R., Roth J., Neville D.M. Insulin interaction with liver plasma membranes. Indipendence of binding of the hormone and its degradation. J. Biol. Chem., 247: 3953, 1972.
Gambhir K.K., Nerurkar S.G., Das P.D., Archer J.A., Henry W.C. Insulin binding and degradation by human erythrocytes at physiological temperature. Endocrinology 109: 1787,1981.
Bellomo G., Bellazzi R., Nicotera P.L., Francesconi R., Fratino P. Binding and degradation of 125l-insulin in human erythrocytes. Comparative studies with hemolysate and membranes. J. Endocrinol. Invest. 5: 287,1982.
Boyoum A. A one stage procedure for isolation of granulocytes from human blood. Scand. J. Clin. Lab. Invest., 21 (Suppl. 97): 51,1968.
Gambhir K.K., Archer J.A., Bradley C.J. Characteristics of human erythrocytes insulin receptors. Diabetes 27: 701,1978.
Dodge J.T., Mitchell C., Hamahn D.J. The preparation and chemical characteristic of hemoglobin-free ghost of human erythrocytes. Arch. Biochem. Biophys. 100: 119,1963.
Lowry H.O., Rosebrough R.J., Farr A.L., Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem., 53: 265,1958.
Lux S.E. Dissecting the Red Cell Membrane Skeleton Nature 281: 426,1979.
Engel P.C. Enzyme kinetics Halsted Press Division of John Wiley and Sons, Inc. New York, 1977.
Gammeltoft S., Gliemann J. Binding and degradation of 125I-labelled insulin by isolated rat fat cells. Biochim. Biophys. Acta 320: 16, 1972.
Hammond J.M., Jarret C. Insulin degradation by isolated fat cells and they subcellular fractions. Diabetes 24: 1011,1975.
Suzuki K., Kono T. Internalization and degradation of fat cell bound insulin. J. Biol. Chem., 254: 9786, 1979.
Dial L.K., Mivamoto S., Arquilla E.R. Modulation of 125I-insulin degradation by receptors in liver plasma membranes. Biochim. Biophys. Res. Commun. 74: 545,1977.
Lang U., Kahn C.R., Chrambach A. Characterization of the insulin receptor and insulin degrading activity from human lymphocytes by quantitative polycrylamide gel electrophoresis. Endocrinology 106: 40,1980.
Pontremoli S., Salamino F., Sparatore B., Melloni E., Morelli A., Benotti U., De Flora A. Isolation and partial characterization of three acidic proteinase in erythrocyte membranes. Biochem. J., 787:559, 1979.
Tokes Z.A., Chambers S.M. Proteolitic activity associated with human erythrocyte membranes. Self-digestion of isolated human erythrocyte membranes. Biochem. and Biophys. Acta 389: 325,1975.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bellomo, G., Nicotera, P.L., Parini, A. et al. Insulin degradation-in human erythrocytes. Effect of Triton X-100 treatment on insulin-degrading activity of membranes. J Endocrinol Invest 6, 441–444 (1983). https://doi.org/10.1007/BF03348343
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF03348343