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Reciprocal effects of an acute load of thyroxine or triiodothyronine on their peripheral metabolism and deiodination in the cold-acclimated rat

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Abstract

Wistar rats acclimated to cold received iv 25μg T4/100g bw, 21 μg T3/100g bw or 2.5 μg T3/100g bw, to observe the changes induced in the peripheral metabolism of 125I-T4 and 125I-T3 relative to cold-adapted untreated animals. Each animal was injected with a tracer dose of either labelled hormone in addition to the T4 or T3 load, and placed in metabolic cages for 24 hour collection of urine and feces. Sequential heparinized blood samples were obtained by cardiac puncture. A T4 load decreased the deiodination of 125I-T4 and 125I-T3 (p < 0.01) as revelated by urinary 125I. A T3 load, in the two dosages employed, decreased the deiodination of 125I-T3(p < 0.01) but had no effect on deiodination of 125I-T4. Similarly, a T4 load increased the fecal excretion of both radioactive iodothyronines (p < 0.01) whereas a T3 load failed to alter the excretion of 125I-T4. In cold-adapted animals plasma TSH was elevated (p < 0.05) and plasma T4 was low (p < 0.001) as compared to rats housed at 22 C. It is concluded that the relative contribution of T4 and T3 to the metabolic state in the rat is not significantly altered by cold exposure.

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Authors and Affiliations

  1. Endocrine Research Laboratory, CONICET. Center of Nuclear Medicine, Hospital de Clínicas José de San Martín, University of Buenos Aires and the Atomic Energy Commission, C.C. 29 Sue 53, 1453, Buenos Aires, Argentina

    R. Boado (Research Fellow), E. Ulloa (Research Fellow) &  Angel A. Zaninovich

Authors
  1. R. Boado
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  2. E. Ulloa
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  3. Angel A. Zaninovich
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Supported by Grants 3728d and 3728e/79from CONICET.

Established Investigador. CONICET.

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Boado, R., Ulloa, E. & Zaninovich, A.A. Reciprocal effects of an acute load of thyroxine or triiodothyronine on their peripheral metabolism and deiodination in the cold-acclimated rat. J Endocrinol Invest 5, 217–221 (1982). https://doi.org/10.1007/BF03348326

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