Abstract
To examine androgenic potential of polycystic ovaries (PCO), slices of follicular and stromal tissues from the same ovary obtained by wedge resection from two markedly, two moderately and one slightly enlarged PCO were incubated separately with [1-14C] acetate. Incorporation into progestins, androgens and estrogens was assessed by reverse dilution technique with recrystallization to constant specific activity. Although the greatest incorporation into androstenedione with much lesser incorporation into testosterone and dehydroepiandros-terone was observed with all the five follicles, the amount of incorporation into the three steroids increased gradedly with histologically defined magnitude of thecal cell hyperplasia in atretic follicles. Only the stromal tissues from two markedly enlarged PCO produced androgens with a similar pattern of 14C distribution among the steroids, thereby incorporation into the three androgens remaining 5.5% or less of that by the follicles from the same ovary. Preoperative levels of plasma androstenedione and urinary 17-ketosteroids were shown to increase in four patients with PCO containing atretic follicles with thecal cell hyperplasia, but not in one patient with slightly enlarged PCO containing atretic follicles without thecal cell hyperplasia. It is inferred that atretic follicles with thecal cell hyperplasia is a significant source of androgen overproduction by PCO.
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References
Yen S.S.C. The polycystic ovary syndrome. Clin. Endocrinol. (Tokyo) 12: 177, 1980.
Serio M., Dell’Acqua S., Calagresi E., Borrelli D. Androgen secretion by the human ovary: Measurement of androgens in ovarian vein blood. In: James V.H.T., Serio M., Giusti G. (Eds.), The endocrine function of the human ovary. Academic Press, New York, 1976, p. 471.
McNatty K.P., Makris A., DeGrazia C., Osathanondh R., Ryan K.J. The production of progesterone, androgens, and es- trogens by granulosa cells, thecal tissue, and stromal tissue from human ovaries in vitro. J. Clin. Endocrinol. Metab. 49: 687, 1979.
Rice B.F., Savard K. Steroid hormone formation in the human ovary. IV. Ovarian stromal compartment; formation of radioactive steroids from acetate-1-14C and action of gonadotropins. J. Clin. Endocrinol. Metab. 26: 593, 1966.
McNatty K.P., Smith D.M., Makris A., DeGrazia C., Tulchinsky D., Osathanondh R., Schiff I., Ryan K.J. The intraovarian sites of androgen and estrogen formation in women with normal and hyperandrogenic ovaries as judged by in vitro experiments. J. Clin. Endocrinol. Metab. 50: 755, 1980.
Short R.V. Further observations on the defective synthesis of ovarian steroids in the Stein-Leventhal syndrome. J. Endocrinol. 24: 359, 1962.
Shippel S. The ovarian thecal cell. IV. The hyperthecosis syndrome. J. Obstet. Gynaecol. Brit. Emp. 62: 321, 1955.
Fienberg R. Thecosis: A study of diffuse stromal thecosis of the ovary and superficial collagenization with follicular cysts (Stein-Leventhal ovary). Obstet. Gynecol. 21: 687, 1963.
Ryan K.J., Petro Z., Kaiser J. Steroid formation by isolated and recombined ovarian granulosa and thecal cells. J. Clin. Endocrinol. Metab. 28: 355, 1968.
Tsang B.K., Moon Y.S., Simpson C.W., Armstrong D.T. Androgen biosynthesis in human ovarian follicles: Cellular source, gonadotropic control, and adenosine 3′, 5′-monophosphate mediation. J. Clin. Endocrinol. Metab. 48: 153, 1979.
Stein I.F., Leventhal M.L. Amenorrhea associated with bilateral polycystic ovaries. Am. J. Obstet. Gynecol. 29: 181, 1935.
Bardin C.W., Lipsett M.B. Testosterone and androstenedione blood production rates in normal women and women with idiopathic hirsutism or polycystic ovaries. J. Clin. Invest. 46: 891, 1967.
Patton W.C., Berger M.J., Thompson I.E., Chong A.P., Grimes E.M., Taymor M.L. Pituitary gonadotropin responses to synthetic luteinizing hormone-releasing hormone in patients with typical and atypical polycystic ovary disease. Am. J. Obstet. Gynecol. 121: 382, 1975.
Mori T., Fujita Y., Suzuki A., Kinoshita Y., Nishimura T., Kambegawa A. Functional and structural relationships in steroidogenesis in vitro by human ovarian follicles during maturation and ovulation. J. Clin. Endocrinol. Metab. 47: 955, 1978.
Savard K., Marsh J.M., Rice B.F. Gonadotropins and ovarian steroidogenesis. Recent Prog. Horm. Res. 21: 285, 1965.
Himelstein-Braw R., Byskov A.G., Peters H., Fabor M. Follicular atresia in the infant human ovary. J. Reprod. Fertil. 46: 55, 1976.
Mori T., Fujita Y., Nihnobu K., Ezaki Y., Kubo K., Nishimura T. Steroidogenesis in vitro by human ovarian follicles during the process of atresia. Clin. Endocrinol. (Tokyo) (In press).
Mori T. Steroid hormone formation in human ovarian follicles in vitro. Endocrinol. Jpn. 23: 365, 1976.
Judd H.L., Scully R.E., Herbst A.L., Yen S.C.C., Ingersol F.M., Kliman B. Familial hyperthecosis: Comparison of endocrinologic and histologic findings with polycystic ovarian disease. Am. J. Obstet. Gynecol. 117: 976, 1973.
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Mori, T., Fujita, Y., Nihnobu, K. et al. Significance of atretic follicles as the site of androgen production in polycystic ovaries. J Endocrinol Invest 5, 209–215 (1982). https://doi.org/10.1007/BF03348325
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DOI: https://doi.org/10.1007/BF03348325