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The effects of relaxin on the mouse mammary gland. II the epithelium

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Abstract

The study deals with the effects of relaxin (RLX) on epithelial cells of the mammary ducts in ovariectomized virgin mice, evaluated by light and electron microscopy. One μg (3 GPU) pure porcine RLX (NIH-RXN-P1) was administered either alone or after pretreatment with estrogen. The possibility of a non specific binding of RLX to insulin receptors on the mammary epithelial cells was excluded by comparing the effects of the administration of the two hormones at the same doses. Our findings indicate that RLX greatly enhances the growth of the mammary duct epithelial cells, as demonstrated by the extensive branching and elongation of the ducts, and by the rather frequent occurrence of mitoses in the epithelial cell precursors and of the intermediate stages of their differentiation into mature duct cells. Three GPU RLX, administered alone, had only an incostant and slight effect on the epithelial cell growth, a priming dose with estrogen being necessary to obtain the highest levels of duct growth. Moreover the treatment with 1 μg of insulin for the same time showed a very inconstant and lesser effect in comparison to RLX. We did not find any evidence of alveolar maturation and milk secretion in the RLX-treated mice. The recognition of a stimulatory role of RLX on the proliferation and growth of the mammary duct epithelium may be of a certain importance in the understanding of hormonal control of the mammary development in physiological conditions and the genesis and growth of mammary neoplasms.

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References

  1. Hamolsky M., Sparrow R.C. Influence of relaxfn on mammary development in sexually immature female rats. Proc. Soc. Exp. Biol. Med. 60: 8, 1945.

    Article  PubMed  CAS  Google Scholar 

  2. Trentin J.J. Relaxin and mammary growth in the mouse. Proc. Soc. Exp. Biol. Med. 78: 9, 1951.

    Article  PubMed  CAS  Google Scholar 

  3. Garret F.A., Talmage R.V. The influence of relaxin on mammary gland development in guinea-pigs and rabbits. J. Endocrinol. 8: 336, 1952.

    Article  Google Scholar 

  4. Smith T.C. The action of relaxin on mammary gland growth in the rat. Endocrinology 54: 59, 1954.

    Article  PubMed  CAS  Google Scholar 

  5. Wada H., Turner C.W. Effect of relaxin on mammary gland growth in female mice. Proc. Soc. Exp. Biol. Med. 101: 707, 1959.

    Article  CAS  Google Scholar 

  6. Wright L.C., Anderson R.R. Effect of relaxin on mammary growth in the hypophysectomized rat. In: Anderson R.R. (Ed.), Relaxin. Advances in experimental Medicine and Biology. Plenum Press, New York and London, vol. 143, 1982, p. 341.

    Google Scholar 

  7. Zarrow M.X., McClintock J.A. Localization of 131 I-labelled antibody to relaxin. J. Endocrinol. 36: 377, 1966.

    Article  PubMed  CAS  Google Scholar 

  8. McMurtry J.P., Kwok S.C.M., Bryant-Greenwood G.D. Target tissues for relaxin identified in vitro with 125I-labelled porcine relaxin. J. Reprod. Fertil. 53: 209, 1978.

    Article  PubMed  CAS  Google Scholar 

  9. Bani G., La Malfa A., Petrucci F., Bigazzi M. Effects of relaxin on mammary gland growth in female mice. In: Bigazzi M., Greenwood F.C., Gasparri F. (Eds.), Biology of relaxin and its role in the human. Excerpta Medica, Amsterdam, 1983, p. 106.

    Google Scholar 

  10. Bani G., Bigazzi M. Morphological changes induced on mouse mammary gland by porcine and human relaxin. Acta Anat. 119: 149, 1984.

    Article  PubMed  CAS  Google Scholar 

  11. Steinetz B.G., Beach V.L., Kroc R.L., Stasilli N.R., Nussbaum R.E., Nemith P.J., Dun R.K. Bioassay of relaxin using a reference standard: A simple and reliable method utilizing direct measurement of interpubic ligament formation in mice. Endocrinology 67: 102, 1960.

    Article  PubMed  CAS  Google Scholar 

  12. Bani G., Bigazzi M., Bani D. Effects of relaxin on the mouse mammary gland. I. The myoepithelial cells. J. Endocrinol. Invest. 8: 207, 1985.

    Article  PubMed  CAS  Google Scholar 

  13. Mayer G., Klein M. Histology and cytology of the mammary gland. In: Kon S.K., Cowie AT. (Eds.), Milk: the mammary gland and its secretion. Academic Press, New York, vol. 1, 1961, p. 47.

    Google Scholar 

  14. Sekhri K.K., Pitelka D.R., De Orne K.B. Studies of mouse mammary glands. I. Cytomorphology of the normal mammary gland. J. Natl. Cancer Inst. 39: 459, 1967.

    PubMed  CAS  Google Scholar 

  15. Girardie J. Histocytomorphologie de la glande mammaire de la souris C3H et de trois autres roungeurs. Z. Zellforsch. 87: 478, 1968.

    Article  PubMed  CAS  Google Scholar 

  16. Salazar H., Tobon H. Morphologic changes of the mammary gland during development, pregnancy and lactation. In: Josimovich J.B., Reynolds M., Cobo E. (Eds.), Lactogenic hormones, fetal nutrition and lactation. John Wiley and Sons, New York, 1974, p. 221.

    Google Scholar 

  17. Fanger H., Ree H.J. Cyclic changes of human mammary gland epithelium in relation to the menstrual cycle — An ultrastructural study. Cancer 34: 574, 1974.

    Article  Google Scholar 

  18. Reece R.P. Mammary gland development and function. In: Velardo J.T. (Ed.), The endocrinology of reproduction. University Press, New York, 1958, p. 213.

    Google Scholar 

  19. Pitelka D.R., Hamamoto S.T., Duafala J.G., Nemanik M.K. Cell contact in the mouse mammary gland. I. Normal gland in postnatal development and the secretory cycle. J. Cell Biol. 56: 797, 1973.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  20. Nardi E., Bigazzi M., Agrimonti F., Dogliotti L., Massi G.B., Ferrari A.M., Ciardetti P., De Luca V., Angeli A. Relaxin and fibrocystic disease of the mammary gland. In: Bigazzi M., Greenwood F.C., Gasparri F. (Eds.), Biology of relaxin and its role in the human. Excerpta Medica, Amsterdam, 1983, p. 417.

    Google Scholar 

  21. Plunkett E.R., Gammal E.B. The effect of relaxin upon DMBA-induced mammary cancer in female rats. Br. J. Cancer 21: 592, 1967.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  22. Segaloff A. The role of the ovary in the synergism between radiation and estrogen in the production of mammary cancer in the rat. In: Bigazzi M., Greenwood F.C., Gasparri F. (Eds.), Biology of relaxin and its role in the human. Excerpta Medica, Amsterdam, 1983, p. 410.

    Google Scholar 

  23. Benson G.K., Cowie A.T., Cox C.P., Flux D.S., Folley S.J. Studies on the hormonal induction of mammary growth and lactation in the goat. 2. Functional and morphological studies of hormonal developed udders with special reference of the effect of ‘triggering’ doses of oestrogen. J. Endocrinol. 13: 46, 1955.

    Article  PubMed  CAS  Google Scholar 

  24. Lyons W.R., Li C.H., Johnson R.E. The hormonal control of mammary growth and lactation. Recent Prog. Horm. Res. 14: 219, 1958.

    PubMed  CAS  Google Scholar 

  25. Nandi S. Hormonal control of mammogenesis and lactogenesis in C3H/HeCrgl mouse. University California Publ. Zool. 65: 1, 1959.

    Google Scholar 

  26. Ceriani R.L. Hormones and other factors controlling growth in the mammary gland. A review. J. Invest. Dermatol. 63: 93, 1974.

    Article  CAS  Google Scholar 

  27. Porter J.C. Hormonal regulation of breast development and activity. J. Invest. Dermatol. 63: 85, 1974.

    Article  PubMed  CAS  Google Scholar 

  28. Harness J.R., Anderson R.R. Effect of relaxin oh mammary gland growth and lactation in the rat. Proc. Soc. Exp. Biol. Med. 148: 933, 1975.

    Article  PubMed  CAS  Google Scholar 

  29. Sheffield L.G., Anderson R.R. Effect of estradiol and relaxin on growth of fibroblastic cells isolated from guinea pig mammary glands. Biol. Reprod. 30: 338, 1984.

    Article  PubMed  CAS  Google Scholar 

  30. Mercado-Simmen R.C., Bryant-Greenwood G.D., Greenwood F.C. Relaxin receptors in the rat myometrium: regulation by estrogen and relaxin. Endocrinology 110: 220, 1982.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Animal Biology and Genetics, University of Florence, Florence, Italy

    G. Bani

  2. Endocrine Unit, I.N.R.C.A., Florence, University of Florence, 50100, Florence, Italy

    M. Bigazzi

  3. Institute of Histology and General Embryology, University of Florence, 50100, Florence, Italy

    D. Bani

  4. Dipartimento di Biologia animale e Genetica, Laboratorio di Istologia, Via Romana 17, 50125, Firenze, Italy

    G. Bani

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  1. G. Bani
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  2. M. Bigazzi
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  3. D. Bani
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Bani, G., Bigazzi, M. & Bani, D. The effects of relaxin on the mouse mammary gland. II the epithelium. J Endocrinol Invest 9, 145–152 (1986). https://doi.org/10.1007/BF03348086

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  • DOI: https://doi.org/10.1007/BF03348086

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