Abstract
Late-onset 21-hydroxylase deficiency (21OHD) presents biochemical evidence of 21OHD and virilization in peri-or postpubertal age; it has been demonstrated that late-onset 21OHD is linked to HLA system. We present the HLA typing, the baseline and the ACTH-stimulated hormonal Ievelsin5 patients with late-onset 21 OHD and in their family members. We identified 3 HLA identical male sibs within their respective families, 2 sibs sharing one haplotype with the affected member and 2 homozygous normal sibs. We observed elevated baseline (> 4 ng/ml) and ACTH-stimulated 17-hydroxyprogesterone levels, increased baseline Androstenedione levels, slightly elevated or normal DHEA-S and Testosterone values and subnormal response of Cortisol levels to AGTH in patients and in the HLA-identical sibs, reduced SHBG levels in patients but not in their identical sibs. The heterozygous family members presented hyperresponsiveness of 17-hydroxyprogesterone but not of androgens after ACTH. We confirm that late-onset 21 OHD is an autosomal recessive disease linked to HLA-B; there is in fact biochemical evidence of mild 21 OHD in patients and in their HLA identical sibs and 17-hydroxyprogesterone levels in the range of heterozygotes for classical 21 OHD in parents and sibs predicted by HLA to be carriers. Thus HLA typing and hormonal data, particularly 17-hydroxyprogesterone, are useful, also in this form of congenital hyperplasia, in detecting heterozygotes.
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Blankstein J., Faiman C., Reyes F.I., Schroeder M.L., Winter J.S.D. Adult-onset familiar adrenal 21 -hydroxylase deficiency. Am. J. Med. 68: 441, 1980.
Rosenwaks Z., Lee P.A., Jones G.S., Migeon C.J., Wentz A.C. An attenuated form of congenital virilizing adrenal hyperplasia. J. Clin. Endocrinol. Metab. 49: 335, 1979.
New M.I., Lorenzen F., Pang S., Gunczler P., Dupont B., Levine L.S. “Acquired” adrenal hyperplasia with 21 -hydroxylase deficiency is not the same genetic disorder as congenital adrenal hyperplasia. J. Clin. Endocrinol. Metab. 48: 356, 1979.
Kauschansky A., Kaufman H., Zamir R., Elian E. Late-onset adrenal hyperplasia (21-hydroxylase deficiency): 17-hydroxyprogesterone response to ACTH stimulation and HLA typing. Horm. Res. 14: 73, 1981.
Bouchard P., Kutten F., Mowszowicz I., Schaison G., Raux-Eurin M.C., Mauvais-Jarvis P. Congenital adrenal hyperplasia due to partial 21-hydroxylase deficiency, a study of five cases. Acta Endocrinol. (Kbh.) 96: 107, 1981.
Dupont B., Oberfield S.E., Smithwick E.M., Lee T.D., Levine L.S. Close genetic linkage between HLA and congenital adrenal hyperplasia (21 -hydroxylase deficiency). Lancet 2: 1309, 1977.
Kohn B., Levine L.S., Pollack M.S., Pang S., Lorenzen F., Levy D., Lerner A.J., Rondanini G.F., Dupont B., New M.I. Late-onset steroid 21 -Hydroxylase deficiency: a variant of classical congenital adrenal hyperplasia. J. Clin. Endocrinol. Metab. 55: 817, 1982.
Migeon C.J., Rosenwaks Z., Lee P.A., Urban M.D., Bias W.B. The attenuated form of congenital adrenal hyperplasia as an allelic form of 21 -hydroxylase deficiency. J. Clin. Endocrinol. Metab. 51: 647, 1980.
Lee P.A., Rosenwaks Z., Urban M.D., Migeon C.J., Bias W.D. Attenuated form of congenital adrenal hyperplasia due to 21 -hydroxylase deficiency. J. Clin. Endocrinol. Metab. 55: 866, 1982.
Laron Z., Pollack M.S., Zamir R., Roitman A., Dickerman Z., Levine L.S., Lorenzen F., O’Neill G.J., Pang S., New M.I., Dupont B. Late-onset 21-hydroxylase deficiency and HLA in the Ashkenazi population: a new allele at the 21 -hydroxylase locus. Hum. Immunol. 1: 155, 1980.
Terasaky P.I., McClelland J.D. Microdroplet assay of human serum cytotoxins. Nature 204: 998, 1964.
Chetkowskì R.J., deFazio J., Shamonki I., Judd H.L., Chang R.J. The incidence of late-onset congenital adrenal hyperplasia due to 21 -hydroxylase deficiency among hirsute women. J. Clin. Endocrinol. Metab. 58: 595, 1984.
Gourmelen M., Pham Huu Trung M.T., Bredon M.G., Girard F. 17-hydroxyprogesterone in the Cosyntropin test: results in normal and hirsute women and in mild congenital adrenal hyperplasia. Acta Endocrinol. (Kbh.) 90: 481, 1979.
Weil J., Bidlingmaier F., Sippel W.G., Butenandl O., Knorr D. Comparison of two tests for heterozygosity in congenital adrenal hyperplasia. Acta Endocrinol. (Kbh.) 91: 109, 1979.
Levine L.S., Dupont B., Lorenzen F., Pang S., Pollack M., Oberfield S., Kohn B., Lerner A., Cacciari E., Mantero F., Scaroni C., Chiumello G., Rondanini G.F., Gargantini L., Giovannelli G., Virdis R., Bartolotta E., Migliori C., Pintor C., Tato L., Barboni F., New M.I. Cryptic 21 -hydroxylase deficiency in families of patients with classical congenital adrenal hyperplasia. J. Clin. Endocrinol. Metab. 51: 1312, 1980.
Petersen K.E., Svejgaard A., Nielsen M.D., Dissing J. Heterozygotes and cryptic patients in families of patients with congenital adrenal hyperplasia (21-hydroxylase deficiency). Horm. Res. 16: 151, 1982.
Boué A., Couillin P., Pomarede R., Rappaport R., Boué J. Génétique du déficit en 21 -hydroxylase. Ann. Endocrinol. (Paris) 43: 3, 1982.
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Scaroni, C., Orlandini, E., Pasini, C.V. et al. HLA and hormonal studies in 5 patients with late-onset 21-hydroxylase deficiency syndrome (21 OHDS). J Endocrinol Invest 9, 65–70 (1986). https://doi.org/10.1007/BF03348067
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DOI: https://doi.org/10.1007/BF03348067