Abstract
Gangiiosides are known to play an important role in different cell processes. We have investigated the effect of TSH on [14C] glucosamine incorporation into gangiiosides of cultured pathological cells isolated from colloid nodular goiter, follicular adenoma, and toxic adenoma. The effect of thyroid stimulating immunoglobulins (TSI) has also been similarly studied on primary cultures from colloid nodular goiter and from porcine thyroid cells. Immunoglobulins were purified from sera of Graves’ disease patients. Cells were cultured with or without either stimulator and labelled with [14C] glucosamine during 24 h. Total lipid extracts were fractionated on DEAE Sephadex A 25. Eluted gangiiosides were analysed by thin layer chromatography, detected by autoradiography and scraped off for radioactivity measurement. Radioactivity was related to the free cholesterol content. The ganglioside pattern of human thyroid cells in primary cultures is found to vary and to closely reflect the ganglioside pattern of the corresponding gland. No ganglioside pattern could be related to a specific thyroid disorder. When cells are treated with TSH, the amount of [14C] glucosamine incorporated in total gangliosides varies according to diseases. The radioactivity incorporated in gangliosides from colloid nodular goiter cells is not similarly affected by TSH and TSI. In porcine thyroid cells, TSH and TSI have the same effect on glucosamine incorporation into gangliosides. Thus it appears that TSH and TSI may differently affect the gangliosides labelling according to the origin of the thyroid cells.
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References
Hakompri S.I. Glycosphingolipids in cellular interaction, differentiation and oncogenesis. Ann. Rev. Biochem. 50: 733, 1981.
Monsigny M., Kieda C., Roche A.C. Membrane glycoproteins glycolipids and membrane lectins as recognition signals in normal and malignant cells. Biol. Cell. 47: 95, 1983.
Ando S. Gangliosides in the nervous system. Neurochemistry Internal. 5: 507, 1983.
Cuatrecasas P. Interaction of Vibrio Cholerae enterotoxin with cell membranes. Biochemistry 12: 3547, 1973.
Grollman E.F., Lee G., Ramos S., Lazo P., Kaback H.R., Friedman R.M., Kohn L.D. Relationship of the structure and function of the interferon receptor to hormone receptors and establishment of the antiviral state. Cancer Res. 38: 4172, 1978.
Mullin B.R., Fishman P.H., Lee G., Aloj S., Ledley F.D., Winand R.J., Kohn L.D., Brady R.O. Thyrotropin-ganglioside interactions and their relationship to the structure and function of thyrotropin receptors. Proc. Natl. Acad. Sci. USA. 73: 842, 1976.
Gardas A., Nauman J. Presence of gangliosides in the structure of membrane receptor for thyrotropin. Acta Endocrinol. (Kbh.). 98: 519, 1981.
Dufrane S.P., Poss A., Goormaghtigh E., Ruysschaert J.M. Cholesterol mediates thyrotropin binding to liposomes containing gangliosides. Experientia 37: 524, 1981.
Dacremont G., DeBaets M., Kaufman J.M., Elewaut A., Vermeulen A. Inhibition of adenylate cyclase activity of human thyroid membranes by gangliosides. Biochem. Biophys. Acta 770: 142, 1984.
Dumont J.E., Lamy F. The regulation of thyroid cell metabolism, function, growth, and differentiation. In: De Visscher M. (Ed.), The thyroid gland. Raven Press, New York, 1980, p. 153.
Beckner S.K., Brady R.O., Fishman P.H. Reevaluation of the role of gangliosides in the binding and action of thyrotropin. Proc. Natl. Acad. Sci. USA. 78: 4848, 1981.
Gosselin L.E., Etienne-Decerf J., Winand R. Triggering by thyrotropin of an increased synthesis of triglycerides in cultured human thyroid cells. Biochem. Biophys. Res. Commun. 97: 391, 1980.
Winand R.J., Kohn L.D. Thyrotropin effects on thyroid cells in culture. J. Biol. Chem. 250: 6534, 1975.
Etienne-Decerf J., Winand R. A sensitive technique for determination of thyroid-stimulating immunoglobulin (TSI) in unfractionated serum. Clin. Endocrinol. (Oxf.) 14: 83, 1981.
Joustra M.J., Lundgren M. Preparation of freeze-dried monomeric and immunochemically pure IgG by a rapid and reproducible Chromatographic technique. In: Protides of Biological Fluids. Proceedings of the 17th Colloquium, Bruges, Belgium. Pergamon Press, Oxford, 1970.
Lowry O.M., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265, 1951.
Laine R.A., Stellner K., Hakomori S.I. Isolation and characterization of membrane glycosphingolipids. In: Korn E.D. (Ed.)., Methods in membrane biology. Plenum Press, New York, 1974, vol. 2, p. 205.
Ledeen R.W., Yu R.K., Eng L.F. Gangliosides of human myelin: Sialosyl galactosylceramide as a major component. J. Neurochem. 21: 829, 1973.
Svennerholm L. Quantitative estimation of sialic acids. Colorimetric resorcinol hydrochloric acid method. Biochem. Biophys. Acta 24: 604, 1957.
Fewster M.E., Burns B., Mead J.F. Quantitative densitometric thin layer chromatography of lipids using copper acetate reagent. J. Chromatogr. 43: 120, 1969.
Franc J.L., Hovsepian S., Fayet G., Bouchilloux S. Differential effects of thyrotropin on various glycosyltransferases in porcine thyroid cells. Biochem. Biophys. Res. Commun, 118: 910, 1984.
Wadeleux P.A., Winand R.J. Some unexpected effects of thyrotropin on the metabolism of thyroid cells isolated from non toxic goiter. Acta Endocrinol. (Kbh.) 98: 533, 1981.
Holmes S.D., Gitlin J., Titus G., Field J.B. Effect of increased circulating thyroid-stimuiation hormone on in vitro thyroid hormone stimulation of thyroid and adipose tissues. Endocrinology 106: 1892, 1980.
Witte A., Henderson B., Zakarija M., McKenzie J.M. Thyrotropin binding to rat thyroid membranes: reduced capacity associated with goitrogenesis. Endocrinology 106: 1489, 1980.
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Etienne-Decerf, J., Gosselin-Rey, C., Gosselin, L. et al. Effects of thyrotropin and thyroid stimulating immunoglobulins on gangliosides labelling of human thyroid cultured pathological cells. J Endocrinol Invest 9, 57–63 (1986). https://doi.org/10.1007/BF03348065
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DOI: https://doi.org/10.1007/BF03348065