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Thyroid hormones homeostasis in rats refed after short-term and prolonged fasting

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Abstract

Effects of starvation on thyroid hormone homeostasis were usually determined after 2 or more days of fasting, however, both in man and in rodents, natural feeding cycles comprise far shorter fasting periods. Therefore serum levels of T4, FT4, T3, FT3 and rT3 were measured in rats refed chow diet for 1, 4, 8 or 24 hours after 14 or 48 hours of starvation. Both short-term (14 h) and long-term fasting (48 h) decreased body weight and serum glucose level. Short-term fast decreased serum FT3 and did not change serum levels of T4, FT4, T3 and rT3. Total T3 and reverse T3 increased after one and 4 hours, free T3 after 4 hours and total T4 after 4 and 8 hours of refeeding. Percent of FT3 did not change after short-term fast, declined after 1 and 4 hours of refeeding, and normalised thereafter. Prolonged starvation (48 h) decreased serum T4, T3, FT3 and % FT3 with no changes in FT4 and rT3. After 24 hours of refeeding only FT3 and % FT3 returned to control levels while total T4 and total T3 were still diminished, and reverse T3 levels did not change. The results suggest that the length of preceding fasting period may strongly influence thyroid hormone homeostasis during fasted-to-fed transition.

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References

  1. Danforth E. Jr. Effects of fasting and altered nutrition on thyroid hormone metabolism in man. In: Henneman G. (Ed.), Thyroid hormones metabolism. Dekker, New York, 1986, p. 353.

    Google Scholar 

  2. Hugues J.N., Burger A.G., Pekary I.A., Hershman J.M. Rapid alteration of serum thyrotropin, triiodothyronine and reverse triiodothyronine levels to short-term starvation and refeeding. Acta Endocrinol. (Copenh.) 105: 194, 1984.

    CAS  Google Scholar 

  3. Blake N.G., Eckland D.J.A., Foster O.J.F., Lightman S.L Inhibition of hypothalamic thyrotropin-releasing hormone messenger ribonucleic acid during food deprivation. Endocrinology 129: 2714, 1991.

    Article  PubMed  CAS  Google Scholar 

  4. Mori M., Michimata T., Yamada M., Yamaguchi M., Irichijima T., Cobayashi S. Food deprivation decreased the blood TRH concentration in the rat. Exp. Clin. Endocr. 91: 97, 1988.

    Article  CAS  Google Scholar 

  5. Rondeel J.M.M., Heide R., de Greef W.J., van Toor H., Klootwijk W., Visser T.J. Effect of starvation and subsequent refeeding on thyroid functionand release of hypothalamic Thyrotropin-Releasing-Hormone. Neuroendocrinology 56: 348, 1992.

    Article  PubMed  CAS  Google Scholar 

  6. Harris A.R.C., Fang S.L., Aziz F., Lipworth L, Vagenakis A.G., Braverman L.E. Effect of starvation on hypothalamic-pituitary thyroid function in rat. Metabolism 27: 1074, 1978.

    Article  PubMed  CAS  Google Scholar 

  7. Harris A.R.C., Fang S.L., Vagenakis A.G., Braverman L.E. Effect of starvation, nutriment replacement, and hypothyroidism on in vitro hepatic T4 to conversion in the rat. Metabolism 27: 1680, 1978.

    Article  PubMed  CAS  Google Scholar 

  8. Hugues J.N., Enjalbert A., Burger A.G., Voirol M-J., Sebaoun J., Epelbaum J. Sensitivity of thyrotropin (TSH) secretion to 3, 5,3′-triiodothyronine and TSH-Releasing Hormone in rat during starvation. Endocrinology 119: 253, 1986.

    Article  PubMed  CAS  Google Scholar 

  9. Kaplan M.M., Utiger R.D. lodothyronine metabolism in rat liver homogenates. J. Clin. Invest. 61: 459, 1978.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  10. Campbell G.A., Kurz M., Marshall S. Effects of starvation in rats on serum levels of follicle stimulating hormone, luteinizing hormone, thyrotropin, growth hormone and thyrotropin releasing hormone. Endocrinology 100: 580, 1977.

    Article  PubMed  CAS  Google Scholar 

  11. Rodriguez M., Rodriguez F., Jolin T. Effect of restricted feeding, fasting, and diabetes on the relationship between thyroid hormone receptor occupancy, growth hormone induction, and inhibition of thyrotropin release in thyroidectomized rats. Endocrinology 131: 1612, 1992.

    PubMed  CAS  Google Scholar 

  12. Balsam A., Ingbar S.H., Sexton F. The influence of fasting diabetes and several pharmacological agents on the pathways of thyroxine metabolism in rat liver. J. Clin. Invest. 62: 415, 1978.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  13. Nishida M., Pittman CS. Glucose-evoked recovery of hepatic thyroxine 5′-deiodinase independent of de novo protein synthesis in fasted rat. Experientia 46: 67, 1989.

    Article  Google Scholar 

  14. Mysliwski A., Kmiec Z. Effect of aging on glycogen synthesis in liver of starved-refed rats. Arch. Gerontol. Geriatr. 14: 85, 1992.

    Article  PubMed  CAS  Google Scholar 

  15. Werner W., Rey H., Wielinger H. Ein neuer Reagent zur enzymatischen Bestimmung von Glucose mit Glucose-oxidase Methode. Fresenius’ Z. Anal. Chem. 252: 224, 1970.

    Article  CAS  Google Scholar 

  16. Dauncey M.J., Ramsden D.B., Kapadi A.L., Macari M., Ingram D.L. Increase in serum concentrations of 3,5,3′-tri-iodothyronine and thyroxine after a meal, and its dependence on energy intake. Horm. Metab. Res. 15: 499, 1983.

    Article  PubMed  CAS  Google Scholar 

  17. Dubuc P.U. Thyroid hormone responses to feeding in ob/ob mice. Horm. Metab. Res. 21: 546, 1989.

    Article  PubMed  CAS  Google Scholar 

  18. Cohen J.H., Ales S., DeVito W.J., Braverman L.E., Emerson C.H. Fasting associated changes in serum thyrotropin in the rat are influenced by the gender. Endocrinology 124: 3025, 1989.

    Article  PubMed  CAS  Google Scholar 

  19. Young R.A., Rajatanavin R., Moring A.F., Braverman L.E. Fasting induces the generation of serum thyronine-binding globulin in Zucker rats. Endocrinology 116: 1248, 1985.

    Article  PubMed  CAS  Google Scholar 

  20. Burger A.G., Berger M., Wimpfheimer K., Danforth E. Interrelationships between energy metabolism and thyroid hormone metabolism during starvation in the rat. Acta Endocrinol. 93: 322, 1980.

    PubMed  CAS  Google Scholar 

  21. Hugues J.N., Burger A.G., Grouselle D., Voirol M-J., Chabert P., Modigliani E., Sebaoun J. Evidence of a thyrothropin releasing hormone (TRH) dependent increase in plasma thyrotropin (TSH) during refeeding of starved rats. Endocrinology 112: 715, 1983.

    Article  PubMed  CAS  Google Scholar 

  22. Young R.A., Braverman L.E, Rajatanavin R. Low protein-high carbohydrate diet induces alterations in the serum thyronine-binding proteins in the rat. Endocrinology 110: 1607, 1982.

    Article  PubMed  CAS  Google Scholar 

  23. Jordan D., Rousset B., Perrin F., Fournier M., Orgiazzi J. Evidence for circadian variations in serum thyrotropin, 3,5,3′-triiodothyronine, and thyroxine in the rat. Endocrinology 107: 1245, 1980.

    Article  PubMed  CAS  Google Scholar 

  24. Rookh H.V., Azukizawa M., DiStefano III J.J., Ogihara T., Hershman J.M. Pituitary-thyroid hormone periodicities in serially sampled plasma of unanesthetized rats. Endocrinology 104: 851, 1979.

    Article  PubMed  CAS  Google Scholar 

  25. Garcin H., Higuret P. Free and protein bound triiodothyronine in the serum of vitamin A deficient rats. J. Endocrinol. 84: 135, 1980.

    Article  PubMed  CAS  Google Scholar 

  26. Glass A.R., Vigersky R.A., Rajatanavin R., Pardrige W., Smallridge R.C., Wartofsky L, Burman K.D. Low serum thyroxine and high serum triiodothyronine in nephrotic rats: etiology and implications for bioavailibility of protein-bound hormone. Endocrinology 114: 1745, 1984.

    Article  PubMed  CAS  Google Scholar 

  27. Wade S., Bleiberg-Daniel F., Le Moullac B. Rat transthyretin: effects of acute short-term food deprivation and refeeding on serum and cerebrospinal fluid concentration and on hepatic mRNA level. J. Nutrition 118: 199, 1988.

    CAS  Google Scholar 

  28. Chopra I.J. Alterations in monodeiodination of iodothyronines in the fasting rat: effects of reduced nonprotein sulfhydryl groups and hypothyroidism. Metabolism 29: 161, 1980.

    Article  PubMed  CAS  Google Scholar 

  29. Kinlaw W.B., Schwartz H.L., Oppenheimer H. Decreased serum triiodothyronine in starving rats is due primarily to diminished thyroidal secretion of thyroxine. J. Clin. Invest. 75: 1238, 1985.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  30. DiStefano J.J. III, Thuvan T., Nguyen T., Yi-Meng Y. Direct measurement of whole body thyroid hormone pool sizes and interconversion rates in fasted rats: hormone regulation implications. Endocrinology 134: 1700, 1993.

    Google Scholar 

  31. De Jong M., Docter R., Van der Hoek H.J., Vos R.A., Krenning E.P., Hennemann G. Transport of 3,5,3′-triiodothyronine into the rerfused rat liver and subsequent metabolism are inhibited by fasting. Endocrinology 131: 463, 1992.

    PubMed  Google Scholar 

  32. Kuhn E.R., Bellon K., Huybrechts L, Heyns W. Endocrine differences between the Wistar and Sprague-Dawley laboratory rat: influence of cold adaptation. Horm. Metab. Res. 15: 491, 1983.

    Article  PubMed  CAS  Google Scholar 

  33. Galan X., Llobera M., Ramirez I. Lipoprotein lipase and hepatic lipase in Wistar and Sprague-Dawley rat tissues. Differences in the effects of gender and fasting. Lipids 29: 333, 1994.

    CAS  Google Scholar 

  34. Panov A., Solovyov V., Vavilin V. Interstrain differences in organization of metabolic processes in the rat liver. I. The dynamics of changes in the contents of adenine nucleotides, glycogen and fatty acyl-CoAs in the course of short-term starvation in the livers of rats of Wistar, August and WAG strains. Int. J. Biochem. 23: 875, 1991.

    Article  PubMed  CAS  Google Scholar 

  35. Refetoff S., Nicoloff J.T. Thyroid hormone transport and metabolism. In: L.J. DeGroot (Ed.), Endocrinology. W.B. Saunders, Philadelphia, 1995, p. 560.

    Google Scholar 

  36. Visser T.J. Importance of deiodination and conjugation in the hepatic metabolism of thyroid hormone. In: Greer M.A. (Ed ), The thyroid Gland. Raven Press, New York, 1990, p. 255.

    Google Scholar 

  37. LoPresti J.S., Gray D., Nicoloff J.T. Influence of fasting and refeeding on 3,3′,5′- triiodothyronine in man. J. Clin. Endocrinol. Metab. 72: 130, 1991.

    Article  PubMed  CAS  Google Scholar 

  38. Heber D. Endocrine responses to starvation, malnutrition, and illness. In: DeGroot L.J. (Ed.), Endocrinology. W.B. Saunders, Philadelphia, 1995, vol. 3, p. 2663.

    Google Scholar 

  39. Munday M.R., Milic M.R., Takhar S., Holness M.J., Sugden M.C. The short-term regulation of hepatic acetyl-CoA carboxylase during starvation and re-feeding in the rat. Biochem. J. 280: 733, 1991.

    PubMed Central  PubMed  CAS  Google Scholar 

  40. Liverini G., Iossa S., Barletta A. Relationship between resting metabolism and hepatic metabolism: effect of hypothyroidism and 24 hours fasting. Horm. Res. 38: 154, 1992.

    Article  PubMed  CAS  Google Scholar 

  41. O’Mara B.A., Dittrich W., Lauterio T.J., St-Germain D.L Pretranslational regulation of type I 5′-deiodinase by thyroid hormones and in fasted and diabetic rats. Endocrinology 133: 1715, 1993.

    PubMed  Google Scholar 

  42. Gardner D.R., Kaplan M.M., Stanley C.A., Utiger R.D. Effect of triiodothyronine replacement on the metabolic and pituitary responses to starvation. N. Engl. J. Med. 300: 579, 1979.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Dept. Histology and Immunology, Medical University of Gdansk, ul. Debinki 1, 80–210, Gdansk, Poland

    Z. Kmiec, G. Kotlarz, B. Smiechowska & A. Mysliwski

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  1. Z. Kmiec
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  2. G. Kotlarz
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  3. B. Smiechowska
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  4. A. Mysliwski
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Kmiec, Z., Kotlarz, G., Smiechowska, B. et al. Thyroid hormones homeostasis in rats refed after short-term and prolonged fasting. J Endocrinol Invest 19, 304–311 (1996). https://doi.org/10.1007/BF03347867

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