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Insulin signaling in adipose tissue of patients with primary aldosteronism

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Abstract

Objectve: We studied phosphorylation of insulin-receptors substrate downstream molecules: 1) in the ex-vivo visceral adipose tissue (VAT) of patients with aldosterone-producing adenoma (APA) (no.=7) and non-functioning adenoma (NFA) (no.=7) undergoing laparoscopic adrenalectomy; 2) in aldosterone-treated sc adipocytes of subjects (no.=5) who requested abdominoplasty. Patients and methods: Western blotting was used to detect phosphorylation of Akt and extracellular signal-regulated kinase (ERK) 1/2 in VAT from APA and NFA patients, and in subcutaneous adipocytes pre-treated with different aldosterone concentrations. Phosphorylation of Akt and ERK1/2 was similar in VAT of patients with APA and NFA. Pre-treatment in adipocytes with both physiological (1 nM) and pharmacological (10 μM) doses of aldosterone did not affect basal or insulin-induced phosphorylation of Akt and ERK1/2. Conclusions: Our data give further evidence that insulin signaling in human VAT is not affected by primary aldosterone overproduction.

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Authors and Affiliations

  1. Department of Medical and Surgical Sciences, University of Padova, Padova, Italy

    R. Urbanet, C. Pilon, R. Vettor & F. Fallo MD

  2. Department of Emergency and Organ Transplantation, Section of Internal Medicine, Endocrinology, Andrology and Metabolic Diseases, University of Bari, Bari, Italy

    F. Giorgino

Authors
  1. R. Urbanet
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  2. C. Pilon
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  3. F. Giorgino
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  4. R. Vettor
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  5. F. Fallo MD
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Correspondence to F. Fallo MD.

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Urbanet, R., Pilon, C., Giorgino, F. et al. Insulin signaling in adipose tissue of patients with primary aldosteronism. J Endocrinol Invest 34, 86–89 (2011). https://doi.org/10.1007/BF03347035

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  • DOI: https://doi.org/10.1007/BF03347035

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