Effect of licorice on the reduction of body fat mass in healthy subjects


The history of licorice, as a medicinal plant, is very old and has been used in many societies throughout the millennia. The active principle, glycyrrhetinic acid, is responsible for sodium retention and hypertension, which is the most common side-effect. We show an effect of licorice in reducing body fat mass. We studied 15 normalweight subjects (7 males, age 22–26 yr, and 8 females, age 21–26 yr), who consumed for 2 months 3.5 g a day of a commercial preparation of licorice. Body fat mass (BFM, expressed as percentage of total body weight, by skinfold thickness and by bioelectrical impedance analysis, BIA) and extracellular water (ECW, percentage of total body water, by BIA) were measured. Body mass index (BMI) did not change. ECW increased (males: 41.8±2.0 before vs 47.0±2.3 after, p<0.001; females: 48.2±1.4 before vs 49.4±2.1 after, p<0.05). BFM was reduced by licorice: (male: before 12.0±2.1 vs after 10.8±2.9%, p<0.02; female: before 24.9±5.1 vs after 22.1±5.4, p<0.02); plasma renin activity (PRA) and aldosterone were suppressed. Licorice was able to reduce body fat mass and to suppress aldosterone, without any change in BMI. Since the subjects were consuming the same amount of calories during the study, we suggest that licorice can reduce fat by inhibiting 11β-hydroxysteroid dehydrogenase Type 1 at the level of fat cells.

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  1. 1.

    Edwards CRW, Stewart PM, Burt D, et al. Localization of 11-hydroxysteroid dehydrogenase-tissue specific protector of the mineralocorticoid receptor. Lancet 1988, 2: 986–9.

    PubMed  Article  CAS  Google Scholar 

  2. 2.

    Funder JW, Pearce P, Smith R, Smith AI. Mineralcorticoid action: target tissue specificity is enzyme, not receptor mediated. Science 1988, 242: 583–5.

    PubMed  Article  CAS  Google Scholar 

  3. 3.

    Stewart PM and Krozowski ZS. 11β-hydroxysteroid dehydrogenase. Vitam Hormon 1999, 57: 249–324.

    Article  CAS  Google Scholar 

  4. 4.

    Quinkler M, Oelkers W, Diederhich S. Clinical implications of glucocorticoid metabolism by 11β-hydroxysteroid dehydrogenase in target tissues. Europ J Endocrinol 2001, 144: 87–97.

    Article  CAS  Google Scholar 

  5. 5.

    Sandeep TC, Walker BR. Pathophysiology of modulation of local glucocorticoid levels by 11β-hydroxysteroid dehydrogenases. Trends Endocrinol Metab 2001, 12: 446–53.

    PubMed  Article  CAS  Google Scholar 

  6. 6.

    Bujalska IW, Kumar S, Stewart PM. Does central obesity reflect “Cushing’s distese of the omentum”? Lancet 1997, 349: 1210–3.

    PubMed  Article  CAS  Google Scholar 

  7. 7.

    Bujalska IW, Kumar S, Hewison M, Stewart PM. Differentiation of adipose stromal cells: the roles of glucocorticoids and 11β- hydroxysteroid dehydrogenase. Endocrinology 1999, 140: 3188–96.

    PubMed  CAS  Google Scholar 

  8. 8.

    Stewart PM and Tomlinson JW. Cortisol, 11β-hydroxysteroid dehydrogenase Type 1 and central obesity. Trends Endocrinol Metab 2002, 13: 94–6.

    PubMed  Article  CAS  Google Scholar 

  9. 9.

    Whorwood CB, Sheppard MC, Stewart PM. Licorice inhibits 11β-hydroxysteroid dehydrogenase messanger ribonucleic acid levels and potentiates glucocorticoid hormone action. Endocrinology 1993, 132: 2287–92.

    PubMed  CAS  Google Scholar 

  10. 10.

    Krahenbuhl S, Hasler F, Frey BM, et al. Kinetics and dynamics of orally administered 18β-glycyrrhetinic acid in humans. J Clin Endocrinol Metab 1994, 78: 581–5.

    PubMed  CAS  Google Scholar 

  11. 11.

    Farese RV Jr., Biglieri EG, Irony I, Gomes Fontes R. Licorice-induced hypermineralocorticoidism. N Engl J Med 1991, 325: 1223–7.

    PubMed  Article  Google Scholar 

  12. 12.

    Pratesi C, Scali M, Zampollo V, et al. Effects of licorice on urinary metabolites of cortisol and cortisone. J Hypertens 1991, 9: S274–5.

    Article  CAS  Google Scholar 

  13. 13.

    Armanini D, Lewicka S, Pratesi C, et al. Further studies on the mineralocorticoid action of licorice in humans. J Endocrinol Invest 1996, 19: 624–9.

    PubMed  CAS  Article  Google Scholar 

  14. 14.

    Armanini D, Wehling M, Weber PC. Mineralocorticoid effector mechanism of liquorice derivates in human leukocytes. J Endocrinol Invest 1989, 12: 303–6.

    PubMed  CAS  Article  Google Scholar 

  15. 15.

    Armanini D, Karbowiak I, Funder JW. Affinity of liquorice derivatives for mineralocorticoid and glucocorticoid receptors. Clin Endocrinol (Oxf) 1983, 19: 609–12.

    Article  CAS  Google Scholar 

  16. 16.

    Armanini D, Bonanni G, Palermo M. Reduction of serum testosterone in men by licorice. N Engl J Med 1999, 341: 1158.

    PubMed  Article  CAS  Google Scholar 

  17. 17.

    Armanini D, Fiore C, Mattarello MJ, Bielenberg J, Palermo M. History of the endocrine effects of licorice. Exp Clin Endocrinol Diabetes 2002, 110: 257–61.

    PubMed  Article  CAS  Google Scholar 

  18. 18.

    Hort A. Theophrastus: Enquiry into plants and minor works on odours an weather signs Heinemann. Cambridge Mass Harvard University Press, 1949, vol II Buch IX, Kap 132.

    Google Scholar 

  19. 19.

    Kumagai A, Nishino K, Yamamoto M, Nanaboshi M, Yamamura Y. An inhibitory effect of glycyrrhizin on metabolic actions of cortisone. Endocrinol Jpn 1966, 13: 416–9.

    PubMed  Article  CAS  Google Scholar 

  20. 20.

    Yamamoto M, Takeuchi M, Kotani S, Kumagai A. Effect of glycyrrhizin and cortisone on cholesterol metabolism in the rat. Endocrinol Jpn 1970, 17: 339–48.

    PubMed  Article  CAS  Google Scholar 

  21. 21.

    Fuhrman B, Volkova N, Kaplan M, et al. Antiatherosclerotic effects of licorice extract supplementation on hypercholesterolemic patients: increased resistance of LDL to atherogenic modification, reduced plasma lipid level, and decreased systolic blood pressure. Nutrition 2002, 18: 268–73.

    PubMed  Article  CAS  Google Scholar 

  22. 22.

    Fuhrman B, Buch S, Vaya J, et al. Licorice extract and its major polyphenol glabridin protect low-density lipoprotein against lipid peroxidation: in vitro and ex vivo studies in humans and in atherosclerotic apolipoprotein E-deficient mice. Am J Clin Nutr 1997, 66: 267–75.

    PubMed  CAS  Google Scholar 

  23. 23.

    Kiso Y, Tohkin M, Hikinoi H, Hattori M, Sakamoto T, Namba T. Mechanism of antihepatotoxic activity of glycyrrhizin, I: effect on free radical generation and lipid peroxidation. Planta Medica 1984, 15: 298–302.

    Article  Google Scholar 

  24. 24.

    Bernardi M, D’Intino PE, Trevisani F. et al. Effect of prolonged ingestion of graded doses of licorice by healthy volunteers. Life Sci 1994, 55: 863–72.

    PubMed  Article  CAS  Google Scholar 

  25. 25.

    Durnin JW, Rahman MM The assessement of the amount of fat in the human body from measurement of skinfold thickness. Br J Nutr 1967, 21: 681–9.

    PubMed  Article  CAS  Google Scholar 

  26. 26.

    Durnin JVGA, Womerseley J. Body assessment from total body and women aged from 17 to 72 years. Br J Nutr 1974, 32: 77–97.

    PubMed  Article  CAS  Google Scholar 

  27. 27.

    Visser M, Deurenberg P, van Staveren WA. Multi-frequency bioelectrical impedance for assessing total body water and extracellular water in elderly subjects. Eur J Clin Nutr 1995, 49: 256–66.

    PubMed  CAS  Google Scholar 

  28. 28.

    Kyle UG, Genton L, Hans D, Karsegard L, Slosman DO, Pichard C. Age-related differences in fat-free mass, skeletal muscle, body cell mass and fat mass between 18 and 94 years. Eur J Clin Nutr 2001, 55: 663–72.

    PubMed  Article  CAS  Google Scholar 

  29. 29.

    Palermo M, Gomes-Sanches C, Roitmann E, Shackleton CHL. Quantitation of cortisol and related 3-oxo-4ene steroids in urine using gas chromatography/mass spectrometry with stable isotope-labeled internal standards. Steroids 1996, 61: 583–9.

    PubMed  Article  CAS  Google Scholar 

  30. 30.

    Beretta-Piccoli C, Salvadé G, Crivelli PL, Weidmann P. Body-sodium and blood volume in a patient with licoriceinduced hypertension. J Hypertension 1985, 3: 19–23.

    Article  CAS  Google Scholar 

  31. 31.

    James B. The use of liquorice in weight reduction. Lancet 1956, 10: 996.

    Article  Google Scholar 

  32. 32.

    Scali M, Pratesi C, Zennaro MC, Zampollo V, Armanini D. Pseudohyperaldosteronism from liquorice-containing laxatives. J Endocrinol Invest 1990, 13: 847–8.

    PubMed  CAS  Article  Google Scholar 

  33. 33.

    Tempel DL, McEwen BS, Leibowitz SF. Effects of adrenal steroid agonists on food intake and macronutrient selection. Physiol Behav 1992, 52: 1161–6.

    PubMed  Article  CAS  Google Scholar 

  34. 34.

    Goodfriend TL, Kelley DE, Goodpaster BH, Winters SJ. Visceral obesity and insulin resistance are associated with plasma aldosterone levels in women. Obes Res 1999, 7: 355–62.

    PubMed  Article  CAS  Google Scholar 

  35. 35.

    Colls J, Rees A, Ward C. Identification and monitoring of disordered water balance. Bioelectrical impedance analysis as an alternative to the target weight procedure. Aust N Z J Mental Health Nurs 2000, 9: 177–83.

    Article  CAS  Google Scholar 

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Correspondence to Prof. D. Armanini.

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Armanini, D., De Palo, C.B., Mattarello, M.J. et al. Effect of licorice on the reduction of body fat mass in healthy subjects. J Endocrinol Invest 26, 646–650 (2003). https://doi.org/10.1007/BF03347023

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  • Licorice
  • bioelectrical impedance analysis
  • cortisol
  • cortisone
  • volume expansion
  • fat disposition
  • 11β-hydroxysteroid dehydrogenase Type 1