Abstract
The purpose of this study was to evaluate the increases in plasma levels of E1, E1S and E2 during constant infusion of dehydroepiandrosterone (D) in premenarchal girls (PG) and in normal menstruating women (AW). Unlabelled D was infused for 48 h in 10 premenarchal girls and in 10 normal menstruating women. Plasma levels of D, estrone (E1), estrone sulfate (E1S) and estradiol (E2) were measured before and during 48 h infusion. Metabolic clearance rates of D (MCRD), production rates of D (PRD), and increases in plasma concentration of E1 E1S and E2, relative to the corresponding increase in plasma concentration of D, were determined. The baseline plasma levels of D were found significantly lower in premenarchal girls than in normal menstruating women. MCRD was found significantly higher in premenarchal girls than in normal menstruating women (2565 ± 467 L/day versus 1317 ± 242 L/day, p < 0.01). Administration of D increased the plasma levels of E1 and E1S in the group of normal menstruating women but not in the premenarchal girls. Considering all subjects studied, the increases in plasma levels of E1 and E1S during the infusion of D were significantly correlated with body weight (p< 0.01 and p< 0.001, respectively). From these results, we conclude that in premenarchal girls there is: a) a reduced peripheral contribution of D to E1 and E1S, and/or b) a higher MCRE1 and MCRE1S than in normal menstruating women.
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Feder H.H. Experimental analysis of hormone actions on the hypothalamus anterior pituitary and ovary. In: Adler N.T. (Ed.), Neuroendocrinology on reproduction. Plenum Press, New York and London, 1981, p. 243.
MacDonald P.C., Edman C.D., Kerber I.J., Siiteri P.K. Plasma precursor of estrogen III. Conversion of plasma dehydroisoandrostenedione to estrogen in young non pregnant women. Gynecol. Invest. 7: 165, 1976.
Styne D.M., Grembach M.M. Puberty in male and female: its physiology and disorders. In: Yen S.S.C., Jaffe R.B. (Eds.), Reproductive endocrinology. W.B. Saunders Co, Philadelphia, London, Toronto, 1978, p. 189.
Geigy J.R. Documenta Geigy, Tables scientifiques, ed. 6. Geigy J.R. (Ed.), Geigy J.R., S.A. Basel, 1963, p. 634.
Buster J.E., Abraham G.E. Radioimmunoassay of plasma dehydroepiandrosterone, androsterone. Analyt. Lett. 5: 203, 1975.
Jasonni V.M., Bulletti C., Bolelli G.F., Franceschetti F., Bonavia M., Flamigni C. Estrone sulfate, Estrone, Estradiol concentrations in normal and cirrhotics postmenopausal women. Steroids 41: 569, 1983.
Tait J.F. Review: The use of isotopic steroids for measurement of production rate in vivo. J. Clin. Endocrinol. Metab. 23: 1285, 1963.
Belisle S., Shiff I., Tulchinsky D. The use of constant infusion of unlabelled dehydroepiandrosterone for the assessment of its Metabolic Clearance Rate, its half life and its conversion into estrogens. J. Clin. Endocrinol. Metab. 50: 117, 1980.
Gurpide E. Tracer methods in hormone research. Springer Verlag, New York, Heidelberg, Berlin, 1975, p. 5.
Frish R.E., Revelle R. Height and weight at menarche and a hypothesis of critical body weight and adolescent events. Science 169: 397, 1970.
Grodin J.M., Siiteri P.K., MacDonald P.C. Sources of estrogens production in postmenopausal women. J. Clin. Endocrinol. Metab. 36: 207, 1973.
Siiteri P.K., MacDonald P.C. Role of extraglandular estrogen in human endocrinology. In: Greep R.O., Astwood E. (Eds.), Handbook of Physiology, Section 7, Endocrinology. American Physiological Society, Washington D.C., 1973, Vol.2, p. 615.
Jaffe R.B. The role of ovarian steroids in regulation of hormonal events in human menstrual cycle. In: Givens J.R., Flamigni C. (Eds.), The gonadotropins: basic science and clinical aspects in females. Academic Press, London and New York, 1981, p. 124.
Frish R.E., Revelle R., Cook S. Components of weigth at menarche and the initiation of the adolescent growth spurt in girls: estimated total water, lean body weight and fat. Hum. Biol. 45: 469, 1973.
Jeumiewic N., Brown G.M., Garfinkel P.E., Maldorfsky H. Hypothalamic function as related to body weight and body fat in anorexia nervosa. Psychosom. Med. 40: 187, 1978.
Rosenthal H.E., Pietzak E., Slaunwhite W.R., Sanderberg A.A. Binding of estrone sulfate in human plasma. J. Clin. Endocrinol. Metab. 34: 805, 1972.
Hopper B.R., Yen S.S.C. Circulating concentration of dehydroepiandrosterone sulfate during the puberty. J. Clin. Endocrinol. Metab. 40: 489, 1975.
Zumoff B., Walsh B.T., Katz J.L., Levin J., Rosenfield R.S., Weiner H. Subnormal plasma dehydroepiandrosterone to corticol ratio in anorexia nervosa: A second hormonal parameter of oncogenic regression. J. Clin. Endocrinol. Metab. 56: 668, 1983.
Rich B.H., Rosenfield R.L., Lucky A.W., Heike J.C., Otto P. Adrenarche: Changing adrenal response to adrenocorticotropin. J. Clin. Endocrinol. Metab. 52: 1129, 1981.
Reiter E.O., Fuldamer V.G., Root A.W. Secretion of the adrenal androgen, dehydroepiandrosterone sulfate, during normal infancy, childhood and adolescence, in sick infants and in children with endocrinologic abnormalities. J. Pediatr. 90: 766, 1977.
Stone G. Some in vivo and in vitro interactions of estrogens sulphates with the mouse uterus and vagina. Aust. J. Biol. Sci. 27: 147, 1974.
Dolly J.O., Dodgson K.S., Rose F.A. Studies on the estrogen sulfatase and arylsulfatase C activities of rat liver. Biochem. J. 128: 337, 1972.
Eugell L., Groman E.V. Human placental estradiol dehydrogenase: characterization and structural studies. Recent Prog. Horm. Res. 30: 139, 1974.
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Bulletti, C., Jasonni, V.M., Naldi, S. et al. Reduced conversion of dehydroepiandrosterone into estrogens in premenarchal girls. J Endocrinol Invest 9, 375–381 (1986). https://doi.org/10.1007/BF03346946
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DOI: https://doi.org/10.1007/BF03346946