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A comparison of the direct immunobead test and other tests for sperm antibodies detection

Journal of Endocrinological Investigation volume 14, pages 443–449 (1991)Cite this article

Abstract

The authors report the results of a correlation study between the direct Immunobead Test (d-IBT) and other techniques for antisperm antibodies detection. The Gelatin Agglutination Test (GAT) and Tray Agglutination Test (TAT) were used to detect antibodies in blood serum and seminal plasma (“indirect methods”). The Direct IgG Mixed Antiglobulin Reaction Test (d-MAR test) was used to detect sperm antibodies bound to the sperm surface (“direct method”). A good concordance between the methods, measured by phi and K tests, was found and satisfactory mathematical models were established by regression analyses.

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References

  1. Shulman S., Dondero F. Current Trends in Immunology of Human Reproduction. EOS-J. Immunol. Immunopharmacol. Special Issue, 6, 1986.

  2. Rose N.R., Hjort T., Rumke Ph., Harper M.J.K., Vyazov O. Techniques for detection of iso and auto antibodies to human spermatozoa. Clin. Exp. Immunol. 23: 175, 1976.

    Google Scholar 

  3. Shulman S., Dondero F., Nicotra M. Immunological Factors in Human Reproduction. Academic Press., London-New York, 1982.

    Google Scholar 

  4. Bronson R., Cooper G., Hjort T., Ing R., Jones W.R., Wang S., Mathur S., Williamson H.O., Rust P.F., Fundenberg H.H., Mettler L., Czuppon A.B., Sudo N.V. Anti-sperm antibodies, detected by agglutination, immobilization, microcytotoxicity and immunobeadbinding assays. J. Reprod. Immunol. 8: 279, 1985.

    PubMed  Article  CAS  Google Scholar 

  5. Shulman S. Agglutinating and immobilizing antibodies to spermatozoa. In: Cohen J., Hendry W.P. (Eds.), Spermatozoa, Antibodies and Infertility. Blackwell Scientific Publications Oxford-London, 1978, p. 81.

    Google Scholar 

  6. Meinertz H., Hjort T. Detection of autoimmunity to sperm: mixed antiglobulin reaction (MAR) test or sperm agglutination? A study on 537 men from infertile couples. Fertil. Steril. 46: 86, 1986.

    PubMed  CAS  Google Scholar 

  7. Bronson R., Cooper G.W., Rosenfeld D.L. Ability of antibody-bound human sperm to penetrate zona-free hamster ova in vitro. Fertil. Steril. 36: 778, 1981.

    PubMed  CAS  Google Scholar 

  8. Clarke G.N., Elliott P.J., Smaila C. Detection of sperm antibodies in semen using the immunobead test: a survey of 813 consecutive patients. Am. J. Reprod. Immunol. Microbiol. 7: 118, 1985.

    PubMed  CAS  Google Scholar 

  9. Shulman S., Pretorius E., Keane T. Antibodies to spermatozoa: XI. The use of Immunobeads for the detection of sperm antibodies in serum. Am. J. Reprod. Immunol. Microbiol. 9: 62, 1985.

    PubMed  CAS  Google Scholar 

  10. Lenzi A., Gandini L., Claroni F., Lombardo F., Dondero F. The use of Immunobeads in the diagnosis of male infertility: laboratory study. EOS-J. Immunol. Immunopharmacol. 7: 102, 1987.

    Google Scholar 

  11. Clarke G.N., Stojanoff A., Cauchi M.N. Immunoglobulin class of sperm-bound antibodies in semen. In: K. Bratanov (Ed.), Immunology of Reproduction. Bulgarian Academy of Sciences Press., Sofia, 1982, p. 482.

    Google Scholar 

  12. Kibrick S., Belding D., Merrill B. Methods for the detection of antibodies against mammalian spermatozoa. II. A gelatin agglutination test. Fertil. Steril. 3: 430, 1952.

    PubMed  CAS  Google Scholar 

  13. Friberg J. A simple and sensitive micro-method for demonstration of spermagglutinating activity in serum from infertile men and women. Acta Obstet. Gynecol. Scand. (Suppl.) 36: 21, 1974.

    Article  CAS  Google Scholar 

  14. Jager S., Kremer J., Van Slochteren-Draaisma T. A simple method of screening for antisperm antibodies in the human male. Detection of spermatozoal surface IgG with the direct mixed antiglobulin reaction carried out on untreated fresh human semen. Int. J. Fertil. 23: 12, 1978.

    PubMed  CAS  Google Scholar 

  15. Cerasaro M., Valenti M., Massacesi A., Lenzi A., Dondero F. Correlation between the direct IgG MAR test (mixed antiglobulin reaction test) and seminal analysis in men from infertile couples. Fertil. Steril. 44: 390, 1985.

    PubMed  CAS  Google Scholar 

  16. Colton T. Statistics in Medicine. Little, Brown and Company, Boston, 1979.

    Google Scholar 

  17. Dixon W.J. BMDP Statistical Software. University of California Press, Berkeley, 1985.

    Google Scholar 

  18. Haas G.G. Jr. Clarifying antibody-mediated infertility. Am. J. Reprod. Immunol. Microbiol. 7: 148, 1985.

    PubMed  Google Scholar 

  19. Bronson R.A., Cooper J.W., Rosenfeld D.L. Correlation between regional specificity of antisperm antibodies to the spermatozoa surfaces and complement-mediated sperm immobilization. Am. J. Reprod. Immunol. Microbiol. 2: 222, 1982.

    Article  CAS  Google Scholar 

  20. Clarke G.N., Stojanoff A., Cauchi M.N., Johnson W.I.H. The immunoglobulin class of antispermatozoal antibodies in serum. Am. J. Reprod. Immunol. Microbiol. 7: 143, 1985.

    PubMed  CAS  Google Scholar 

  21. Bronson R.A., Cooper G.W., Rosenfeld D.L. Seminal fluid antisperm antibodies do not reflect those present on the sperm surface. Fertil. Steril. 45: 505, 1987.

    Google Scholar 

  22. Pretorius E., Windt M.L., Menkveld R., Kruger T. Evaluation of immunobead test (IBT), tray agglutination test (TAT), and sperm immobilization test (SIT). Arch. Androl. 20: 159, 1988.

    PubMed  Article  CAS  Google Scholar 

  23. Carson S.A., Reither J., Scommegna A., Prims G.S. Antibody binding patterns in infertile males and females as detected by immunobead test, gelatin agglutination test and sperm immobilization test. Fertil. Steril. 49: 487, 1988.

    PubMed  CAS  Google Scholar 

  24. McClure R.D., Tom R.A., Watkins M., Murthy S. SpermCheck: a simplified screening assay for immunological infertility. Fertil. Steril. 52: 650, 1989.

    PubMed  CAS  Google Scholar 

  25. Hellstrom W.J.C., Samuels S.J., Waits A.B., Overstreet J.W. A comparison of the usefulness of SpermMar test and immunobead tests for detection of antisperm antibodies. Fertil. Steril. 52: 1027, 1989.

    PubMed  CAS  Google Scholar 

  26. Haas G.G., Lambert M., Stern J.E., Manganello P. Comparison of the direct radiolabeled antiglobulin assay and the direct immunobead test for detection of sperm-associated antibodies. AJRI 22: 130, 1990.

    PubMed  Google Scholar 

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Affiliations

  1. Laboratorio di Immunologia della Riproduzione e Seminologia, V Clinica Medica, Università di Roma “La Sapienza”, 00161, Roma, Italy

    Franco Dondero, A. Lenzi, L. Gandini & F. Lombardo

  2. Dipartimento di Medicina Sperimentale, Università di Roma “La Sapienza”, Roma, Italy

    F. Culasso

Authors
  1. Franco Dondero
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  2. A. Lenzi
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  3. L. Gandini
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  4. F. Lombardo
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  5. F. Culasso
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Dondero, F., Lenzi, A., Gandini, L. et al. A comparison of the direct immunobead test and other tests for sperm antibodies detection. J Endocrinol Invest 14, 443–449 (1991). https://doi.org/10.1007/BF03346835

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  • DOI: https://doi.org/10.1007/BF03346835

Key-words

  • Antisperm antibodies
  • immunobead test
  • gelatin agglutination test
  • tray agglutination test
  • spermatozoa
  • fertility
  • immunological infertility