Abstract
Background and aims: The C385A polymorphism of FAAH gene (rs324420C>A) has been associated with obesity. We investigate the role of this polymorphism on anthropometric and insulin resistance responses to a high polyunsaturated fat hypocaloric diet. Methods: Obese individuals (no.=99) were assessed at baseline and after 3 months of a high polyunsaturated fat hypocaloric diet. Results: Seventy-one patients (71.7%) had the genotype C385C and 28 (28.3%) patients had the C385A (26 patients, 26.3%) or A358A (2 patients, 2.0%) (A allele carriers group) genotype. In A allele carriers and after dietary intervention, total cholesterol (−16.3±37.4 mg/dl) and LDL-cholesterol (−12.9±6.5 mg/dl) levels decreased. In subjects with C385C genotype, the decreases were significant in total cholesterol (−12.3±27.4 mg/dl), LDL-cholesterol (−7.5±20.5 mg/dl), insulin (−2.2±6.2 mUI/l), and homeostasis model assessment of insulin resistance (HOMA-R) (−0.79±1.15 units) levels. The weight loss was similar in both genotype groups (−4.1 ±3.8 kg vs −4.2±3.2 kg). Only leptin levels had a significant similar decrease in both genotypes. Conclusion: Subjects with C385C genotype of the FAAH showed an improvement on insulin and HOMA-R levels with a high polyunsaturated fat hypocaloric diet after weight loss during 3 months.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Nguyen DM, El-Serag HB. The epidemiology of obesity. Gastroenterol Clin North Am 2010, 39: 1–7.
Ameri A. The effects of cannabinnoids on the brain. Prog Neurobiol 1999, 58: 315–48.
Ueda N, Puffenbarger RA, Yamamoto S, Deutsch DG. The fatty acid amide hydrolase (FAAH). Chem Phys Lipids 2000, 108: 107–21.
McKinney MK, Cravatt BF. Structure and function of Fatty Acid Amide hydrolase. Annu Rev Biochem 2005, 74: 411–32.
Sipe JC, Chiang K, Gerber AL, Beutler E, Cravatt BF. A Missense mutation in human fatty acid amide hydroxylase associated with problem drug abuse. Proc Natl Acad Sci USA 2002, 99: 8394–9.
Aberle J, Fedderwitz I, Klages N, George E, Beil FU. Genetic variation in two proteins of the endocannabinoid system and their influence on body mass index and metabolism under low fat diet. Horm Metab Res 2007, 39: 395–7.
De Luis DA, Gonzalez Sagrado M, Aller R, Izaola O, Conde R. Effects of C385A missense polymorphism of the endocannabinoid degrading enzyme fatty acid amide hydrolase on weight loss after a hypocaloric diet. Metabolism Clin and Exper 2012, 60: 730–4.
De Luis DA, Gonzalez Sagrado M, Aller R, Izaola O, Conde R. Effects of C358A missense polymorphism of the degrading enzyme fatty acid amide hydrolase on weight loss, adipocytokines, and insulin resistance after 2 hypocaloric diets Metabolism Clin and Exper 2010, 59: 1387–92.
Itariu BK, Zeyda M, Hochbrugger EE, et al. Long-chain n-3 PUFAs reduce adipose tissue and systemic inflammation in severely obese nondiabetic patients: a randomized controlled trial. Am J Clin Nutr 2012, 96: 1137–49.
Duart MJ, Arroyo CO, Moreno JL. Validation of an insulin model for the reactions in RIA. Clin Chem Lab Med 2002, 40: 1161–7.
Mathews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF. Homeostasis model assessment: insulin resistance and beta cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia 1985, 28: 412–4.
Friedewald WT, Levy RJ, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma without use of the preparative ultracentrifuge. Clin Chem 1972, 18: 499–502.
Lukaski H, Johson PE. Assessment of fat-free mass using bioelectrical impedance measurements of the human body. Am J Clin Nutr 1985, 41: 810–7.
Mataix J, Mañas M. Tablas de composición de alimentos españoles. Ed: University of Granada, 2003.
Knoll N, Volckmar AL, Pütter C, et al. The fatty acid amide hydrolase (FAAH) gene variant rs324420 AA/AC is not associated with weight loss in a 1-year lifestyle intervention for obese children and adolescents. Horm Metab Res 2012, 44: 75–7.
Papazoglou D, Panagopoulos I, Papanas N, et al. The FAAH Pro129Thr polymorphism is not associated with severe obesity in Greek Subjects. Horm Metab Res 2008, 40: 907–10.
Jensen D, Andreasen C, Andersen M, et al. The functional Pro129Thr variant of the FAAH gene is not associated with various fat accumulation phenotypes in a population-based cohort of 5, 801 whites. J Mol Med 2007, 85: 445–9.
Sipe JC, Chiang K, Gerber AL, Beutler E, Cravatt BF. A missense mutation in human fatty acid amide hydroxylase associated with problem drug abuse. Proc Natl Acad Sci USA 2002, 99: 8394–9.
Di Marzo V, Goparaju SK, Wang L, et al. Leptin. Regulated endocannabinoids are involved in maintaining food intake. Nature 2001, 410: 822–5.
Juárez-López C, Klünder-Klünder M, Madrigal-Azcárate A, Flores-Huerta S. Omega-3 polyunsaturated fatty acids reduce insulin resistance and triglycerides in obese children and adolescents. Pediatr Diabetes 2013, 14: 377–83.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
de Luis, D.A., Izaola, O., Aller, R. et al. Effects of C358A polymorphism of the endocannabinoid degrading enzyme fatty acid amide hydrolase (FAAH) on weight loss, adipocytokines levels, and insulin resistance after a high polyunsaturated fat diet in obese patients. J Endocrinol Invest 36, 965–969 (2013). https://doi.org/10.1007/BF03346760
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF03346760