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Testosterone responses to intensive interval versus steady-state endurance exercise


Free testosterone (FT) hormonal responses were compared between high-intensity interval exercise (IE) and steady-state endurance exercise (SSE) in endurance trained males (no.=15). IE session was repeated periods of 90-sec treadmill running at 100–110% maximal oxygen uptake (VO2max) and 90-sec active recovery at 40% VO2max for 42–47 min. The SSE session consisted of a continuous 45-min run at 60–65% VO2max. Total work output was equal for each exercise session. A 45-min supine rest control session (CON) was also performed. All three sessions were on separate days. Pre-session (PRE), immediate post-session (POST), and 12-h post-session (12POST) blood samples were collected and used to determine FT, SHBG, LH, 3-α-androstanediol glucuronide (3-α Diol G) and cortisol. Analysis of variance compared IE and SSE biomarker responses to the reference CON session. IE and SSE each caused an increase (p<0.01) in FT, but IE more so than SSE (p<0.05). The 5α-reduc-tase marker 3-α Diol G response at 12POST IE was elevated while FT was reduced (p<0.05); no such change occurred following SSE. These findings suggest IE might produce a more pronounced turnover of FT by androgen sensitive tissue than the SSE form of exercise.

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  1. 1.

    McMurray RG, Hackney AC. The endocrine system and exercise. In: Exercise & Sports Science. Garrett W & Kirkendall D (eds). Philadelphia: Williams & Wilkins Publisher. 2000, 135–62.

    Google Scholar 

  2. 2.

    Brooks GA, Fahey TD, White TP. Neural-endocrine control of metabolism. In: Exercise Physiology: Human Bioenergetics and its Application, 2nd edition. Toronto: Mayfield Publishing. 1996, 156–96.

    Google Scholar 

  3. 3.

    Viru A, Viru M. Biochemical Monitoring of Sport Training. Champaign IL: Human Kinetics Publishing. 2001, 91–142.

    Google Scholar 

  4. 4.

    Joyner MJ, Coyle EF. Endurance exercise performance: the physiology of champions. J Physiol 2008, 586: 35–44.

    PubMed Central  PubMed  Article  CAS  Google Scholar 

  5. 5.

    Laursen PB, Jenkins DG. The scientific basis of high intensity interval training. Sports Med 2002, 32: 53–73.

    PubMed  Article  Google Scholar 

  6. 6.

    Hackney AC, Viru A. Research methodology: endocrinologic measurements in exercise science and sport medicine. J Athletic Training 2008, 43: 631–9.

    Article  Google Scholar 

  7. 7.

    ACSM’s Guidelines for Exercise Testing and Prescription, 8th edition. Baltimore, Maryland: Lippincott, Williams & Wilkins. 2009, 97–154.

  8. 8.

    Hackney AC, Kallman A, Hosick KP, Rubin DA, Battaglini CL. Thyroid hormonal responses to intensive interval versus steady-state endurance exercise sessions. Hormones (Athens) 2012, 11: 54–7.

    Google Scholar 

  9. 9.

    ACSM’s Metabolic Calculations and Handbook. Baltimore, Maryland: Lippincott, Williams & Wilkins. 2007, 35–40.

  10. 10.

    Tietz NW. Clinical Guide to Laboratory Tests. Philadelphia: Saunders Publishing. 1990, 78–130.

    Google Scholar 

  11. 11.

    Levene H. Robust tests for equality of variances. In: Contributions to Probability and Statistics. Olkin I (ed), Palo Alto, CA: Stanford Univ. Press. 1960, 278–92.

    Google Scholar 

  12. 12.

    Derbré F, Vincent S, Maitel B, et al. Androgen responses to sprint exercise in young men. Int J Sports Med 2010, 31: 291–7.

    PubMed  Article  CAS  Google Scholar 

  13. 13.

    Zouhal H, Jacob C, Delamarche P, Gratas-Delamarche A. Catecholamines and the effects of exercise, training and gender. Sports Med 2008, 38: 401–23.

    PubMed  Article  Google Scholar 

  14. 14.

    Ross RK, L Bernstein, RA Lobo, et al. 5-alpha-reductase activity and risk of prostate cancer among Japanese and US white and black males. Lancet 1992, 339: 887–9.

    PubMed  Article  CAS  Google Scholar 

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Correspondence to A. C. Hackney.

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Hackney, A.C., Hosick, K.P., Myer, A. et al. Testosterone responses to intensive interval versus steady-state endurance exercise. J Endocrinol Invest 35, 947–950 (2012).

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  • Exercise endocrinology
  • HIT
  • hormones
  • overtraining
  • stress