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Insulin inhibition of protein degradation in cells expressing wild-type and mutant insulin receptors

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Abstract

The mechanism by which insulin decreases protein degradation is unknown. We examined insulin binding and degradation (125I- [A14]insulin) and protein degradation (3H-leucine labeling) in Chinese hamster ovary (CHO) cells transfected with wild-type (WI) and mutant human insulin receptors. The ΔExon-16 mutant is missing the juxtamembrane domain that mediates endocytosis. The Δ343 mutant receptor lacks the tyrosine kinase structural domain but retains the juxtamembrane internalization domain. The mutant ΔNPEY lacks the single NPEY sequence located 16 residues after the end of the transmembrane domain. Null transfected cells (NEO) not expressing human receptors were studied as controls. The WT and ΔNPEY cells equivalently internalized and degraded insulin; Δ343 cells internalized and degraded insulin, but at a reduced rate; ΔExon-16 cells internalized and degraded significantly less insulin than the other mutants; NEO cells showed essentially no internalization and degradation. In contrast, all cell types showed the same efficacy at inhibition of protein degradation, albeit at different potencies. These results suggest insulin actions are mediated by multiple and redundant effector systems, but that receptor tyrosine kinase activity is not required for inhibition of protein degradation.

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Authors and Affiliations

  1. Research Service, Department of Veterans Affairs Medical Center, University of Nebraska Medical Center, 4101 Woolworth Ave., Omaha, Nebraska, 68105, USA

    F. G. Hamel PhD & R. G. Bennett

  2. Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska, USA

    F. G. Hamel PhD, C. I. Andersen & R. G. Bennett

  3. Department of Veterans Affairs Phoenix, Carl T. Hayden Medical Center, USA

    J. Fawcett & W. C. Duckworth

  4. Molecular and Cellular Biology Program, Arizona State University Tempe, USA

    W. C. Duckworth

  5. Department of Internal Medicine, University of Arizona School of Medicine, Tucson, Arizona, USA

    W. C. Duckworth

  6. Division of Endocrinology, Metabolism and Diabetes, Wayne State, Detroit, Michigan, USA

    P. Berhanu

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  1. F. G. Hamel PhD
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  2. J. Fawcett
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  3. C. I. Andersen
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  4. P. Berhanu
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  5. R. G. Bennett
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  6. W. C. Duckworth
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Correspondence to F. G. Hamel PhD.

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Hamel, F.G., Fawcett, J., Andersen, C.I. et al. Insulin inhibition of protein degradation in cells expressing wild-type and mutant insulin receptors. J Endocrinol Invest 26, 1088–1094 (2003). https://doi.org/10.1007/BF03345255

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  • DOI: https://doi.org/10.1007/BF03345255

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