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Nachweis von Mastzellen mit VPAC2-Rezeptor-Protein in normaler Haut und bei Bullösem Pemphigoid mittels Immunhistochemie

Detection of mast cells with VPAC2 receptor protein in normal skin and bullous pemphigoid using immunohistochemistry

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Zusammenfassung

Nachdem in einer vorangegangenen NeuroMastER-Studie eine Lokalisation der mRNA des V PAC2-Rezeptors für Vasoaktives Intestinales Polypeptid (VIP) erfolgte, war es das Ziel der vorliegenden Studie, in situ Proteindaten zur Lokalisation von V PAC2 bei Bullösem Pemphigoid (BP) zu sammeln. Diese Immunerkrankung unterliegt einer Vielzahl von Umwelteinflüssen.

Mittels unterschiedlicher immunhistochemischer Verfahren, gekoppelt mit Dunkelfeld- und Hellfeld-visualisierenden Techniken, konnte dabei eine Expression von V PAC2-Immunreaktivität in folgenden Zellen von normaler und BP-Haut festgestellt werden: Keratinozyten, Zellen der Gefäßwände, Zellen ekkriner Schweißdrüsen und holokriner Talgdrüsen, Haarfollikelzellen und Zellen der glatten Muskulatur des M. erector pili. Auch Nervenfaserbündel in der Dermis zeigten vereinzelt V PAC2-Immunreaktivität.

Zusammenfassend bestätigen diese Daten auf der translationalen Ebene frühere Studien, die auf der transkriptionellen Ebene VPAC2 mRNA untersucht haben.

V PAC2, VIP und Mastzellen können als wichtige Kandidaten für die Mediation von Prozessen zwischen dem Körper und der Umwelt gelten.

Abstract

After the localisation of mRNA of the V PAC2 receptor of Vasoactive Intestinal Polypeptide (VIP) in a previous NeuroMastER-study, the present study aimed to generate in situ protein data for the localisation of V PAC2 in bullous pemphigoid (BP). This immune disease is subject to numerous environmental influences.

Using different immunohistochemical methods coupled to darkfield and brightfield visualising techniques, the expression of V PAC2 immunoreactivity was found in follwing cells of normal and BP skin: Keratinocytes, cells of vessel walls, eccrine and holocrine glandular cells, hair follicular cells, and cells of the smooth muscle of m. erector pili. Also nerve bundles of the dermis displayed immunoreactivity in part.

In summary, the present data confirm previous transcriptional data on the translational level. V PAC2, VIP and mast cells can be regarded as important candidates for the mediation of interactions between the organism and its environment.

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Literatur

  • Bennett, L. A., Johnson, J. M., Stephens, D. P. et al. (2003). Evidence for a role for vasoactive intestinal peptide in active vasodilatation in the cutaneous vasculature of humans. J. Physiol. 552, 223–232

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Church, M. K., el-Lati, S. & Caulfield, J. P. (1991). Neuropeptide-induced secretion from human skin mast cells. Int. Arch. Allergy Appl. Immunol. 94, 310–318

    Article  CAS  PubMed  Google Scholar 

  • Cordell J.L., Falini B., Erber W.N. et al. (1984). Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal antialkaline phosphatase (APAAP complexes). J Histochem Cytochem. 32(2):219–29

    CAS  PubMed  Google Scholar 

  • Dvorakova, M. C. (2005). Cardioprotective role of the VIP signaling system. Drug News Perspect. 18, 387–391

    Article  CAS  PubMed  Google Scholar 

  • Fizanne, L., Sigaudo-Roussel, D., Saumet, J. L. et al. (2004). Evidence for the involvement of VPAC1 and VPAC2 receptors in pressureinduced vasodilatation in rodents. J. Physiol. 554, 519–528

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Granoth, R., Fridkin, M. & Gozes, I. (2000). VIP and the potent analog, stearyl-Nle(17)-VIP, induce proliferation of keratinocytes. FEBS Lett. 475, 78–83

    Article  CAS  PubMed  Google Scholar 

  • Grant, S., Lutz, E. M., McPhaden, A. R. et al. (2006). Location and function of VPAC1, VPAC2 and NPR-C receptors in VIP-induced vasodilation of porcine basilar arteries. J. Cereb. Blood Flow Metab. 26, 58–67

    Article  CAS  PubMed  Google Scholar 

  • Hartschuh, W., Weihe, E., Yanaihara, N. et al. (1983). Immunohistochemical localization of vasoactive intestinal polypeptide (VIP) in Merkel cells of various mammals: evidence for a neuromodulator function of the Merkel cell. J. Invest Dermatol. 81, 361–364

    Article  CAS  PubMed  Google Scholar 

  • Kurosawa, M. & Ishizuka, T. (1993). Inhibitory effects of vasoactive intestinal peptide on superoxide anion formation by N-formylmethionyl-leucyl-phenylalanine-activated inflammatory cells in vitro. Int. Arch. Allergy Immunol. 100, 28–34

    Article  CAS  PubMed  Google Scholar 

  • Ludolph-Hauser, D., Rueff, F., Sommerhoff, C. P. et al. (1999). Tryptase, a marker for the activation and localization of mast cells. Hautarzt. 50, 556–561

    Article  CAS  PubMed  Google Scholar 

  • Said, S. I. (1996). Molecules that protect: the defense of neurons and other cells. J. Clin. Invest. 97, 2163–2164

    Article  CAS  PubMed Central  PubMed  Google Scholar 

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Authors and Affiliations

  1. Klinische Forschergruppe Allergologie, Charité — Universitätsmedizin, Freie Universität Berlin und Humboldt Universität zu Berlin, Berlin, Deutschland

    David Quarcoo & Beatrix Kloft

  2. Centrum für Anatomie, Charité — Universitätsmedizin, Freie Universität Berlin und Humboldt Universität zu Berlin, Berlin, Deutschland

    Carolin Bester, Marc Ohle & Pia Welker

  3. Klinik für Dermatologie, Allergologie und Venerologie, Charité — Universitätsmedizin, Freie Universität Berlin und Humboldt Universität zu Berlin, Berlin, Deutschland

    Tanja C. Fischer

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  1. David Quarcoo
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  2. Carolin Bester
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  3. Beatrix Kloft
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  4. Marc Ohle
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  5. Pia Welker
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  6. Tanja C. Fischer
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Correspondence to David Quarcoo.

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Quarcoo, D., Bester, C., Kloft, B. et al. Nachweis von Mastzellen mit VPAC2-Rezeptor-Protein in normaler Haut und bei Bullösem Pemphigoid mittels Immunhistochemie. Zbl Arbeitsmed 61, 346–352 (2011). https://doi.org/10.1007/BF03345015

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