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Type I 5′-iodothyronine deiodinase activity and mRNA are remarkably reduced in renal clear cell carcinoma

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Abstract

The purpose of this study was to compare thyroid hormone metabolism between non-cancerous tumor-surrounding human kidney tissues and renal clear cell carcinomas (RCCC). The material consisted of samples taken from 10 RCCC patients of both sexes and three grades of differentiation, G1 to G3. We showed that, similar to rat tissue, type I 5′ monodeiodinase (5′DI) expression is heterogeneous within the human kidney. We also found a poor correlation between 5′DI activity and mRNA level in noncancerous tumor-surrounding tissue suggesting significant post-transcriptional regulation of 5′DI expression by an unidentified process in the human kidney. In all RCCC tissues both 5′DI activity and mRNA levels were undetectable. This suggests either loss of human 5′DI gene expression during neoplastic transformation or the origination of RCCC from a tubular cell type that does not express 5′DI.

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Authors and Affiliations

  1. Department of Internal Medicine and Endocrinology, Medical University of Warsaw, ul. Banacha 1a, 02-097, Warsaw, Poland

    Janusz Pachucki & J. Nauman

  2. Department of Biochemistry, Medical Centre of Postgraduate Education, Ostroleka, Poland

    M. Ambroziak, J. Luczak & A. Nauman

  3. Department of Urology, Regional Hospital, Ostroleka, Poland

    Z. Tanski

  4. Department of Endocrinology, Medical Research Centre, Polish Academy of Science, Warsaw, Poland

    J. Nauman

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  1. Janusz Pachucki
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  2. M. Ambroziak
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  3. Z. Tanski
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  4. J. Luczak
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  5. J. Nauman
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  6. A. Nauman
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Correspondence to Janusz Pachucki.

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Pachucki, J., Ambroziak, M., Tanski, Z. et al. Type I 5′-iodothyronine deiodinase activity and mRNA are remarkably reduced in renal clear cell carcinoma. J Endocrinol Invest 24, 253–261 (2001). https://doi.org/10.1007/BF03343855

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