Abstract
Hyperglycemia is likely to be one of the important determinants of ion transport as it is known to induce oxidative stress and may thus enhance non-specific permeability of membranes. The aim of the present study was to evaluate the effects of an acute increase in glycemia on 86Rb+ (a marker for K+) influx and lipid peroxidation. We evaluated the 75-g oral glucose tolerance test (OGTT)-induced modification on 86Rb+ influx and plasma lipid peroxidation in 20 subjects with normal glucose tolerance (NGT). After 2-hour glucose loading, the levels of passive 86Rb+ influx and plasma lipid peroxidation were significantly increased, whereas the active influx of 86Rb+ was unchanged. The total and passive influx of 86Rb+ into erythrocytes was significantly correlated with the level of plasma lipid peroxidation. This study demonstrates that acute hyperglycemia induces an increase in the passive influx of 86Rb+ in subjects with NGT, suggesting that acute hyperglycemia may produce an oxidative stress in plasma. These changes may be among the earliest changes occurring in response to hyperglycemia.
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Ceriello A. Acute hyperglycemia and oxidative stress generation. Diabet. Med. 1997, 14: S45–S91.
Ceriello A., Motz E., Cavarape A. Hyperglycemia counterbalances the anti-hypertensive effect of glutathione in diabetic patients. Evidence linking hypertension and glycemia through the oxidative stress in diabetes mellitus. J. Diabetes Complications 1997, 11: 250-255.
Ceriello A., Giacomello R., Stel G., Motz E., Taboga C., Tonutti L., Pirisi M., Bartoli E. Hyperglycemia-induced thrombin formation in diabetes. The possible role of oxidative stress. Diabetes 1995, 44: 924–928.
Ceriello A., Falleti E., Motz E., Taboga C., Tonutti L., Ezsol Z., Gonano F., Bartoli E. Hyperglycemia-induced circulating ICAM-1 increase in diabetes mellitus: the possible role of oxidative stress. Horm. Metab. Res. 1998, 30: 146–149.
Ceriello A., Bortolotti N., Crescentini A., Motz E., Lizzio S., Russo A., Ezsol Z., Tonutti L., Taboga C. Antioxidant defences are reduced during the oral glucose tolerance test in normal and non-insulin-dependent diabetic subjects. Eur. J. Clin. Invest. 1998, 28: 329–333.
Konukoglu D., Hatemi H., Ozer E.M., Gönen S., Akcay T. The erythrocyte glutathione levels during oral glucose tolerance test. J. Endocrinol. Invest. 1997, 20: 471–475.
Kaplan P., Matejovicova M., Mezesova V. Iron-induced inhibition of Na+, K(+)-ATPase and Na/Ca2+ exchanger in synaptosomes: protection by the pyridoindole stobadine. Neurochem Res. 1997, 22: 1523–1529.
Jain S.K., McVie R., Duett J., Herbst J.J. Erythrocyte membrane lipid peroxidation and glycosylated hemoglobin in diabetes. Diabetes 1989, 38: 1539–1543.
Novgorodov S.A., Gudz T.I., Kushnareva Y.E., Roginsky V.A., Kudrjashov Y.B. Mechanism accounting for the induction of nonspecific permeability of the inner mitochondrial membrane by hydroperoxides. Biochim. Biophys. Acta 1991, 1058: 242–248.
Onaran I., Yalcin A.S., Sultuybek G. Effect of donor age on the susceptibility of erythrocytes and erythrocyte membranes to cumene hydroperoxide-induced oxidative stress. Mech. Ageing Dev. 1997, 98: 127–138.
Latorre R., Miller C. Conductance and sensitivity in potassium channels. J. Membr. Biol. 1983, 71: 11–30.
Jan L.Y., Jan Y.N. Potassium channels and their evolving gates. Nature 1994, 371: 119–122.
Rajan A.S., Agullar-Bryan L., Nelson D.A., Yaney G.C., Hsu W.H., Konze D.L., Boyd A.E. Ion channels and insulin secretion. Diabetes Care 1990, 13: 340–363.
Sobey W.J., Beer S.F., Carrington C.A. Sensitivity and specific two site immunoradiometric assay for human insulin and proinsulin. Biochem. J. 1989, 260: 535–359.
WHO Study Group. Diabetes mellitus. Technical Report Series, No. 727. WHO, Geneva, 1985.
Conti M., Morand P.C., Levillain P., Lemonnier A. Improved fluorometric determination of malondialdehyde. Clin. Chem. 1991, 37: 1273–1275.
Wolff S.P., Dean R.T. Glucose auto-oxidation and protein modification: The potential role of autooxidative glycosylation in diabetes. Biochem. J. 1987, 245: 243–250.
Ceriello A., Quatraro A., Giugliano D. New insights on non-enzymatic glycosylation may lead to therapeutic approachhes for the prevention of diabetic complications. Diabet. Med. 1992, 9: 297–299.
Cosentino F., Hishikawa K., Katusic Z.S., Lusher T.F. High glucose increases nitric oxide synthase expression and superoxide anion generation in human aortic endothelial cell. Circulation 1997, 96: 25–28.
Baynes J.W. Role of oxidative stress in development of complications in diabetes. Diabetes 1991, 40: 405–412.
Hunt J.V., Smith C.C.T., Wolff S.P. Autoxidative glycosylation and possible involvement of peroxides and free radicals in LDL modification by glucose. Diabetes 1990, 39: 1420–1424.
Mullarkey C.J., Edelstein D., Brownlee M. Free radical generation by early glycation products: a mechanism for accelerated atherogenesis in diabetes. Biochem. Biophys. Res. Commun. 1990, 173: 932–939.
Marfella R., Verrazzo G., Acampora R., La Marca C., Giunta R., Lucarelli C., Paolisso G.C. Glutathione reverses systemic hemodynamic changes induced by acute hyperglycemia in healthy subjects. Am. J. Physiol. Endocrinol. 1995, 268: E1167–E1173.
Jain S.K. Hyperglycemia can cause membrane lipid peroxidation and osmotic fragility in human red blood cells. J. Biol. Chem. 1989, 264: 21340–21345.
Andersson T.G.L., Vinge E. The efflux of 86Rb+ and [3H]5-HT from human platelets during continuous perfusion: effects of potassium-induced membrane depolarization and thrombin stimulation. Acta Physiol. Scand. 1991, 141: 421–428.
Dwight J.F., Hendry B.M. The effects of tert-butyl hydroperoxide on human erythrocyte membrane ion transport and the protective actions of antioxidants. Clin. Chim. Acta 1996, 249: 167–181.
Ney P.A., Christopher T., Hebbel T. Synergistic effects of oxidation and deformation on erythrocyte monovalent cation leak. Blood 1990, 75: 1192–1198.
Satoh K. Serum lipid peroxide in cerebrovascular disorder determined by a new colorimetric method. Clin. Chim. Acta 1978, 90: 37–43.
Gusev G.P., Sherstobitov A.O. Effect of metabolic inhibitors on K+ transport across the lampley erythrocyte membrane. Gen. Physiol. Biophys. 1994, 13: 459–468.
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Güven, M., Onaran, I., Ulutin, T. et al. Effect of acute hyperglycemia on potassium (86Rb+) permeability and plasma lipid peroxidation in subjects with normal glucose tolerance. J Endocrinol Invest 24, 231–235 (2001). https://doi.org/10.1007/BF03343852
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DOI: https://doi.org/10.1007/BF03343852