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Thermodynamic Characterization of Two Nearly Simultaneous Protein Denaturations

  • JCAT′97 28th Conference on Calorimetry and Thermal Analysis Dunkirk, France May 27–30, 1997
  • Published:
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Abstract

Near-UV circular dichroism and differential scanning calorimetry were used to analyse the thermal denaturation of bovine α-lactalbumin at pH 7.5 and various Ca2+ concentrations. Both analyses revealed that the denaturated protein consists of two fractions, a Ca2+-loaded and a Ca2+-free fraction. Despite this agreement, the two methods afforded significantly different values for the thermodynamic parameters of the denaturations. It was demonstrated that the ellipticity changes were more appropriate than the excess heat capacities for analysis of the two types of denaturation. The values of the thermodynamic parameters obtained with the former method are therefore more reliable.

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References

  1. K. R. Acharya, D. I. Stuart, N. R C. Walker, M. Lewis and D. C. Philips, J. Mol. Biol., 208 (1989) 99.

    Article  CAS  Google Scholar 

  2. H. A. McKenzie and F. H. White, Adv. Protein Chem., 41 (1991) 173.

    Article  CAS  Google Scholar 

  3. J. Desmet and F. Van Cauwelaert, Biochim. Biophys. Acta, 912 (1988) 211.

    Article  Google Scholar 

  4. P. Relkin, Crit. Rev. Food Sci. Nutr., 36 (1996) 565.

    Article  CAS  Google Scholar 

  5. P. Haezebrouck, A. De Baetselier, M. Joniau, H. Van Dael, S. Rosenberg and I. Hanssens, Protein Eng., 6 (1993) 643.

    Article  CAS  Google Scholar 

  6. R. Kuroki, K. Nitta and K. Yutani, J. Biol. Chem., 267 (1992) 24297.

    CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Interdisciplinary Research Center, Katholieke Universiteit Leuven Campus Kortrijk, B-8500, Kortrijk, Belgium

    I. Hanssens & G. Vanderheeren

Authors
  1. I. Hanssens
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  2. G. Vanderheeren
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Hanssens, I., Vanderheeren, G. Thermodynamic Characterization of Two Nearly Simultaneous Protein Denaturations. Journal of Thermal Analysis 51, 871–877 (1998). https://doi.org/10.1007/BF03341464

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  • DOI: https://doi.org/10.1007/BF03341464

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