Abstract
The dexamethasone suppression test (DST) is considered an indicator of the function of the adrenal pituitary axis. The effect of the steroid is mediated by its binding to corticosteroid receptors. We previously suggested that the measurement of corticosteroid receptors in lymphocytes is an index of an analogous pattern in brain. In the present study, corticosteroid Type I and Type II receptors in mononuclear leukocytes were measured in 10 elderly subjects and in 9 young adults, before and after overnight DST (1 mg). Receptors were measured by radioreceptor assay. In all the subjects, dexamethasone was able to suppress plasma cortisol. The number of Type I and Type II receptors before the test was lower in elderly subjects than in adults. In the control group, dexamethasone produced a significant depression of Type I receptors (from 267±72 to 169±71 receptors per cell), which can be interpreted as a primary involvement of Type I receptors in the response to dexamethasone; Type II receptors decreased in half the subjects (from 2849±703 to 2345±569 receptors per cell). In elderly healthy subjects, Type II receptors were also significantly decreased (from 1796±671 to 720±345). We suggest that in young subjects Type II receptors are initially up-regulated by dexamethasone, and then down-regulated, while in aged subjects an up-regulation cannot be achieved, as suggested by the higher values of plasma cortisol usually found in aging subjects.
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References
Seeman T.E., Robbins R.J.: Aging and hypothalamic pituitary adrenal response. Endocr. Rev. 15: 233–269, 1994.
Parnetti L., Mecocci P., Neri C., Palazzetti D., Fiacconi M., Santucci A., Santucci C., Ballatori E., Reboldi G.P., Caputo N., Signorini E., Senin U.: Neuroendocrine markers in aging brain: clinical and neurobiological significance of dexamethasone suppression test. Aging Clin. Exp. Res. 2: 173–179, 1990.
Sapolski R.M., Krey L.C., Mc Evans R.M.: The neuroendocrinology of stress and aging: the glucocorticoid cascade hypothesis. Endocr. Rev. 7: 284–301, 1986.
Landfield P.W., Eldridge J.V.C.: The glucocorticoid hypothesis of brain aging and neurodegeneration: recent modifications. Acta Endocrinol. (Copenh.) 125: 54–64, 1991.
Armanini D., Karbowiak I., Scali M., Orlandini E., Zampollo V., Vittadello G.: Corticosteroid receptors and lymphocyte subsets in mononuclear leukocytes in aging. Am. J. Physiol. 282: E464–466, 1992.
Armanini D., Scali M., Vittadello G., Ribecco M., Zampollo V., Pratesi C., Orlandini E., Zovato S., Zennaro M.C., Karbowiak I.: Corticosteroid receptors and aging. J. Steroid Biochem. Molec. Biol. 45: 191–194, 1993.
Armanini D.: Corticosteroid receptors in lymphocytes:a possible marker of brain involution? J. Steroid Biochem. Molec. Biol. 49: 429–434, 1994.
Chrousos G.P.: The hypothalamic-pituitary-adrenal axis and immune-mediated inflammation. N. Engl. J. Med. 332: 1351–1362, 1995.
Bradbury M.J., Akana S.F., Cascio C.S., Levin N., Jacobson L., Dallman M.F.: Regulation of the basal ACTH secretion by corticosterone is mediated by both Type I (MR) and Type II (GR) receptors in rat brain. J. Steroid Biochem. Molec. Biol. 40: 133–142, 1991.
Funder J.W.: Glucocorticoid receptors. J. Steroid Biochem. Molec. Biol. 43: 389–394, 1992.
Lowy M.T.: Corticosterone regulation of brain and lymphoid corticosteroid receptors. J. Steroid Biochem. Molec. Biol. 39: 147–154, 1991.
Pardes E.M., de Vampey J.W., Soto R.J., Mosed D.F., De Nicola A.F.: A correlative study between glucocorticoid receptor levels in human mononuclear leukocytes and biochemical data in Cushing’s disease. Acta Endocrinol. (Copenh.) 120: 55–61, 1989.
Armanini D., Strasser T., Weber P.C.: Characterization of aldosterone binding sites in human mononuclear leukocytes. Am. J. Physiol. 248: E388–E490, 1985.
Armanini D., Strasser T., Weber P.C.: Parallel determination of glucocorticoid receptors in mononuclear and polymorphonuclear leukocytes after Percoll separation. J. Endocrinol. Invest. 8: 45–48, 1985.
Funder J.W., Pearce P., Smith R.: Mineralocorticoid action: target tissue specificity is enzyme not receptor mediated. Science 242: 583–587, 1988.
Armanini D., Kuhnle U., Strasser T., Dorr H., Weber P.C., Butenandt I., Stockigt J., Pearce P., Funder J.W.: Pseudohypoaldosteronism: demonstration of aldosterone receptors deficiency. N. Engl. J. Med. 313: 1178–1181, 1985.
Zennaro M.C., Borensztein M., Jeunemaitre X., Armanini D., Soubrier F.: No alteration in primary structure of the mineralocorticoid receptor in a family with pseudohypoaldosterosnism. J. Clin. Endocrinol. Metab. 79: 32–40, 1994.
Karbowiak I., Scapolan E., Orlandini E., Zovato S., Gottardo C., Basso A., Ferro Milone D., Armanini D.: Corticosteroid receptors in mononuclear leukocytes and lymphocyte subpopulations in Alzheimer’s disease. The Endocrine Society, Washington, June 14–17, 1995, pp. 484.
Zovato S., Simoncini M., Spinella P., Karbowiak I., Spigariol A., Scapolan M., Naaomi O., Zampollo V., Basso A., Armanini D.: Corticosteroid effector mechanism in anorexia nervosa. J. Endocrinol. Invest. 18 (Suppl. 5): 76, 1995.
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Zovato, S., Simoncini, M., Gottardo, C. et al. Dexamethasone suppression test: Corticosteroid receptors regulation in mononuclear leukocytes of young and aged subjects. Aging Clin Exp Res 8, 360–364 (1996). https://doi.org/10.1007/BF03339594
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DOI: https://doi.org/10.1007/BF03339594