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Expression and function of a new angiogenic factor AA98 target molecule at the maternal-embryonic boundary of rhesus monkey

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Chinese Science Bulletin

Abstract

The target molecule of monoclonal antibody AA98 (AA for short) is a new vascular endothelial cell related factor and plays a role in angiogenesis as indicated by the previous data. To investigate its role in angiogenesis and placentation in primate, we examined its expression in the implantation sites on D17, 19, 28 and 34 of gestation in rhesus monkey by immunohistochemistry and Western immunoblot. Western blot analysis showed that the primary antibody used in this study was specific for its epitope. AA protein was mainly expressed in small blood vessels and in some cytotrophoblast cells. The AA staining was found mainly in the endothelial cells and vascular small muscle. This observation supported the AA's role in angiogenesis. AA was spatio-temporarily expressed in cytotrophoblasts: weak in proliferating trophoblast within cell column and endovascular trophoblast, strong in trophoblastic subpopulation within the basal plate and vascular trophoblast; AA staining within the basal plate was down-regulated during early placentation. The shift of AA98 expression in extravillous trophoblasts suggestes a role of this new factor during the course of cytotrophoblast metastasis and spiral artery remodeling. The spatio-temporarily expression indicats that AA98 could be also used as a trophoblast cellular marker to characterize the acquisition of a vascular endothelial and invasive phenotype.

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Authors and Affiliations

  1. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100080, China

    Peng Wei, Zhihong Zhang, Xinlei Chen & Yixun Liu

  2. Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100080, China

    Xiyun Yan

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  1. Peng Wei
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  2. Zhihong Zhang
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  3. Xinlei Chen
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  4. Xiyun Yan
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  5. Yixun Liu
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Correspondence to Yixun Liu.

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Wei, P., Zhang, Z., Chen, X. et al. Expression and function of a new angiogenic factor AA98 target molecule at the maternal-embryonic boundary of rhesus monkey. Chin.Sci.Bull. 48, 881–886 (2003). https://doi.org/10.1007/BF03325669

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  • DOI: https://doi.org/10.1007/BF03325669

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