Abstract
Apoptosis as a mechanism of deleting cells from tissues plays an important role in physiological and varieties of pathological situations, especially cancer conditions. In order to search for tumor cells apoptosis inducers, the inhibition effects on K562 cells of N-phosphoryl dipeptide methyl esters were studied by MTT assays, and (DIPP-L-Leu)2-L-Lys-OCH3 was the compound which had the best activity. From the studies of the typical apoptotic morphologic changes, DNA agarose gel electrophoresis, and flow cytometry analysis, it could be concluded that (DIPP-L-Leu)2-L-Lys-OCH3 could induce apoptosis of K562 cells in a dose-dependent manner, and the IC50 was 22.66 μmol/L according to MTT assays.
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Pérez, J. M, Quiroga, A. G, Montero, E. I. et al., A cycloplatinated compound of p-isopropylbenzaldehyde thiosemicarbazone and its chloro-bridged derivative induce apoptosis in cis-DDP resistant cells which overexpress the H-ras oncogene, Journal of Inorganic Biochemistry, 1999, 73: 235–243.
Kerr, J. F. R, Wyllie, A. H, Currie, A. R., Apoptosis: A basic biological phenomenon with wide ranging implications in tissue kinetics, British Journal of Cancer, 1972, 26: 239–257.
Steller, H., Mechanisms and genes of cellular suicide, Science, 1995, 267: 1445–1449.
Zhao, Y. F, Ju, Y, Li, Y. M. et al., Self-activation of N-phosphoamino acids and N-phosphodipeptides in oligopeptide formation, International Journal of Peptide and Protein Research, 1995, 45: 514–518.
Bertenshaw, S. R, Rogers, R. S, Stern, M. K. et al., Phosphoruscontaining inhibitors of endothelin converting enzyme: Effects of the electronic nature of phosphorus on inhibitor potency, Journal of Medicinal Chemistry, 1993, 36: 173–176.
Doherty, A. M., Endothelin: A new challenge, Journal of Medicinal Chemistry, 1992, 35: 1493–1508.
Kortylewicz, Z. P., Phosphoramidate peptide inhibitors of human skin fibroblast collagenase, Journal of Medicinal Chemistry, 1990, 33: 263–273.
Ji, G. J, Xue, C. B, Zeng, J. N. et al., Synthesis of N-O, O-diisopropyl phosphoryl amino acid and dieptides, Synthesis, 1988, 6: 444–448.
Adachi, S, Cross, A. R, Babior, B. M. et al., Bcl-2 and the outer mitochondrial membrane in the inactivation of cytochrome c during fas-mediated apoptosis, Journal of Biological Chemistry, 1997, 272: 21878–21882.
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Niu, Y., Jiang, Y., Cao, S. et al. Apoptosis induced by (DIPP-L-Leu)2-L-Lys-OCH3 in K562 cells. Chin.Sci.Bull. 48, 869–872 (2003). https://doi.org/10.1007/BF03325666
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DOI: https://doi.org/10.1007/BF03325666