Abstract
Twelve Indian mango cultivars were fingerprinted using anchored-ISSR primers. Out of total 161 bands amplified, 113 (70.2%) were polymorphic, the polymorphism ranging from 50% to 94.1% depending upon the primer. One primer [5′ HVH(CA)7T 3′] with highest genotype index could uniquely identify each of the cultivars studied. Fingerprints based on polymorphic markers amplified by the 10 primers had a 6.75 x 10-12 probability of identical match by chance, indicating a high degree of uniqueness in the anchored-ISSR based fingerprinting. UPGMA dendrogram based on Jaccard’s similarity showed Himayath as the most diverse of the 12 cultivars and the rest of the cultivars clustered into two groups at 0.69 similarity coefficient.
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- ISSR:
-
Inter Simple Sequence Repeats
- PCR:
-
Polymerase Chain Reaction
- RAPD:
-
Random Amplified Polymorphic DNA
- AFLP:
-
Amplified Fragment Length Polymorphism
- SSR:
-
Simple Sequence Repeats
- UPGMA:
-
Unweighted Pair Group Method with Arithmetic Averages
References
Negi SS, Acta Hort, 509 (2000) 69.
Ravishankar KV, Anand L & Dinesh MR, J Hort Sci Biotech, 75 (2000) 198.
Hemanth Kumar NV, Narayanaswamy P, Theertha Prasad D, Mukunda GK & Sondur SN, J Hort Sci Biotech, 76 (2001) 529.
Karihaloo JL, Dwivedi YK, Archak S & Gaikwad AB, J Hort Sci Biotech, 78 (2003) 285.
Saghai-Maroof MA, Soliman KM, Jorgensen RA & Allard RW (1984) Proc Nat Acad Sci, USA, 81 (1984) 8014.
McGregor CE, Lambert, CE, Greyling MM, Louw JH & Warnich L, Euphytica, 113 (2000) 135.
Archak S, Gaikwad AB, Gautam D, Rao E, Swamy K & Karihaloo JL, Euphytica, 230 (2003) 397.
Prevost A & Wilkinson MJ, Theor Appl Genet, 98 (1999) 107.
Powell W, Morgante M Andre C, Hanafey M, Vogel J, Tingey S & Rafalski A, Mol Breed, 2 (1996) 225.
Rohlf FJ, NTSYSpc Numerical Taxonomy and Multivariate Analysis System Version 2.11X. Applied Biostatistics, New York (2000).
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Singh, S., Karihaloo, J.L. & Gaikwad, A.B. DNA Fingerprinting of Some Mango (Mangifera indica L) Cultivars Using Anchored-ISSR Markers. J. Plant Biochem. Biotechnol. 16, 113–117 (2007). https://doi.org/10.1007/BF03321984
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DOI: https://doi.org/10.1007/BF03321984