Abstract
Loss of major mitochondrial complex I subunit Nad 7 in the leaves of CMS II mutant of Nicotiana sylvestris caused increase in both in vivo and in vitro nitrate reductase activities and the per cent activation state of NR was also higher in CMS II as against wild type. The differences suggest a possibility for export of mitochondrial NADH due to impairment of complex I, a major and high affinity sink for NADH. Loss of complex I subunit also resulted in constitutive expression of alternate oxidase (Aox) thereby providing a mechanism for continuous supply of carbon skeletons required for nitrate assimilation. The possibility of close coordination between mitochondrial redox perturbation and nitrate reduction and its further assimilation is discussed.
Abbreviations
- NR:
-
nitrate reductase
- Aox:
-
alternate oxidase
- NRmax:
-
nitrate reductase maximum
- NRact:
-
nitrate reductase actual
References
Crawford NM & Glass ADM, Trends Plant Sci, 3 (1998) 389.
Baurain D, Dinant M, Coosemans N & Rene F Matagne, Plant Physiol, 131 (2003) 1418.
Ramrao CS, Srinivasan & Naik MS, New Phytol, 87 (1981) 517.
Naik MS & Nicholas DJD, Phytochemistry, 25 (1986) 571.
Li XQ, Chetrit P, Mathieu C, Vedal F, Paepe R, Remy R & Ambard-Bretterille F, Curr Genet, 13 (1988) 261.
Jaworski EG, Biochem Biophys Res Commun, 43 (1971) 1274.
Hageman RH & Hucklesby DP, Methods Enzymol, 23 (1971) 491.
Kaiser WM, Kandlbinder A, Stoimenovia M & Clabb J, Planta, 210 (2000) 801.
Heineke D, Reins B, Grosse H, Hoferichter P, Peter U & Flugge UF, Plant Physiol, 95 (1991) 1131.
Dutilleul C, Lelarge C, Prioul JL, Depaepe R, Foyar CH & Noctor G, Plant Physiol, 139 (2005) 64.
Sabar M De Paepe R & de Kouchkovsky Y, Plant Physiol, 124 (2000) 1239.
Naik RM, Mehta SL & Singh P, J Plant Biochem Biotech, 7 (1998) 29.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Naik, R.M. Mitochondrial Complex I Impairment and Nitrate Reductase Activity in Leaves of Nicotiana sylvestris . J. Plant Biochem. Biotechnol. 16, 67–69 (2007). https://doi.org/10.1007/BF03321932
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF03321932