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In Vitro Propagation of a Threatened, Anticarcinogenic Herb, Curculigo orchioides Gaertn

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Abstract

Shoot multiplication of Curculigo orchioides Gaertn (Kali Musali), a threatened medicinal herb, was achieved using shoot bud explants. Single explants cultured on MS + 0.2 mg l−1 BA + 2.5 mg l−1 spermidine produced multiple shoots with an average of 7 shoots per explant that readily rooted on the same medium. Plantlets were acclimatized before successful transfer to soil. The developed protocol demonstrates the use of spermidine for mass propagation that can help in conservation of germplasm of Curculigo orchioides.

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Abbreviations

BA:

6-benzyladenine

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

MS:

Murashige and Skoog’s medium

NAA:

α-naphthaleneacetic acid

spermidine:

N-(3-aminopropyl)butane-1,4-diamine

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Authors and Affiliations

  1. Tissue Culture and Cryopreservation Unit, National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, 110 012, India

    Neelam Sharma, S. Vimala Devi & Ruchira Pandey

Authors
  1. Neelam Sharma
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  2. S. Vimala Devi
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  3. Ruchira Pandey
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Corresponding author

Correspondence to Neelam Sharma.

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Sharma, N., Vimala Devi, S. & Pandey, R. In Vitro Propagation of a Threatened, Anticarcinogenic Herb, Curculigo orchioides Gaertn. J. Plant Biochem. Biotechnol. 16, 63–65 (2007). https://doi.org/10.1007/BF03321931

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  • DOI: https://doi.org/10.1007/BF03321931

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