Genetic Diversity Analysis of Elite Pearl Millet Inbred Lines using RAPD and SSR Markers

  • A. C. Chandra-Shekara
  • B. M. Prasanna
  • S. R. Bhat
  • B. B. Singh
Article

Abstract

Pearl millet [Pennisetum glaucum (L) R Br] is one of the widely grown cereal crops in the arid and semi-arid regions of Africa and India. We undertook a study to ascertain the genetic diversity in 21 elite inbreds (parental lines of 13 pearl millet hybrids in India) using 20 Random Amplified Polymorphic DNA (RAPD) and 21 Simple Sequence Repeat (SSR) markers. Based on Polymorphism Information Content (PIC) and unique banding profiles, 6 RAPD primers OPD12, OPA16, OPB6, OPA19, OPB5 and OPB1, and 3 SSR markers Xpsmp2208, Xpsmp2223 and Xpsmp2220, were found to be highly discriminative. The PIC values ranged from 0.28 to 0.48 for the RAPD and from 0.24 to 0.60 for the SSR markers. Cluster analysis and principal component analysis of the combined dataset of RAPD and SSR markers indicated moderate genetic divergence among the elite pearl millet germplasm, besides unraveling the genetic relationships among the male sterile lines and the restorers.

Key words

DNA fingerprinting genetic diversity Pennisetum glaucum RAPD SSR 

Abbreviations

PCA

Principal Component Analysis

PCR

Polymerase Chain Reaction

GS

Genetic Similarity

PIC

Polymorphism Information Content

RAPD

Random Amplified Polymorphic DNA

SSR

Simple Sequence Repeat

UPGMA

Unweighted Pair Group Method using Arithmetic Averages

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References

  1. 1.
    Budak H, Pedraza F, Cregan PB, Baenziger PS & Dweikat I, Crop Sci, 43 (2003) 2284.CrossRefGoogle Scholar
  2. 2.
    Berwal KK & Khairwal JS, Intl Sorghum Millet Newslett, 38 (1997) 103.Google Scholar
  3. 3.
    Liu CJ, Witcombe JR, Pittaway TS, Nash M, Busso CS, Hash CT & Gale MD, Theor Appl Genet, 89 (1994) 481.Google Scholar
  4. 4.
    Govila OP, In Breeding field crops (VL Chopra, Editor), Oxford & IBH Publishing Co., New Delhi (2001) pp 121–148.Google Scholar
  5. 5.
    Karp A, Edwards K, Bruford M, Vosman B, Morgante M, Seberg O, Klremer A, Boursot P, Alexander P, Tauz D & Hewitt G, Nature Biotech, 15 (1997) 625.CrossRefGoogle Scholar
  6. 6.
    Williams JKF, Kubelik AR, Livak KG, Rafalki JA & Tingey SV, Nucleic Acids Res, 18 (1990) 6531.PubMedCrossRefGoogle Scholar
  7. 7.
    Welsh J & McClelland M, Nucleic Acids Res, 19 (1991) 5275.PubMedCrossRefGoogle Scholar
  8. 8.
    Harris SA, In Molecular systematics and plant evolution (PM Hollingsworth, RM. Bateman, RJ Gornall, Editors), Taylor and Francis, London (1999), pp 211–228.Google Scholar
  9. 9.
    Stojalowski A, Lapinski M & Masojc P, Plant Breed, 123 (2004) 428.CrossRefGoogle Scholar
  10. 10.
    Varshney RK, Graner A & Sorrells ME, Trends Biotech, 23 (2005) 48.CrossRefGoogle Scholar
  11. 11.
    Bertin I, Zhu JH & Gale MD, Theor Appl Genet, 110 (2005) 1467.PubMedCrossRefGoogle Scholar
  12. 12.
    Antunes MS, Vasconcelos MJ & Netto DAM, Intl Sorghum Millet Newslett, 38 (1997) 146.Google Scholar
  13. 13.
    Chowdhari KV, Davierwala AP, Gupta VS, Ranjekar PK & Govila OP, Theor Appl Genet, 97 (1998) 154.CrossRefGoogle Scholar
  14. 14.
    Chowdhari KV, Venkatachalam SR, Davierwala AP, Gupta VS, Ranjekar PK & Govila OP, Theor Appl Genet, 97 (1998) 163.CrossRefGoogle Scholar
  15. 15.
    Mariac C, Luong V, Kapran I, Mamadou A, Sagnard F, Deu M, Chantereau J, Gerard B, Ndjeunga J, Bezançon G, Pham J-L & Vigouroux Y, Theor Appl Genet, 114 (2006) 49.PubMedCrossRefGoogle Scholar
  16. 16.
    Saghai-Maroof MA, Soliman KM, Jorgensen RA & Allard RW, Proc Natl Acad Sci USA, 81 (1984) 8014.PubMedCrossRefGoogle Scholar
  17. 17.
    Chandra-Shekara AC, Prasanna BM, Bhat SR & Singh BB, J Plant Biochem Biotech, 14 (2005) 21.CrossRefGoogle Scholar
  18. 18.
    Qi X, Lindup S, Pittaway TS, Allouis S, Gale MD & Devos KM, Biotechniques, 31 (2001) 355.PubMedGoogle Scholar
  19. 19.
    Nair SK, Prasanna BM, Garg A, Rathore RS, Setty TAS & Singh NN, Theor Appl Genet, 110 (2005) 1384.PubMedCrossRefGoogle Scholar
  20. 20.
    McGregor CE, Lambert CA, Greyliing MM, Louw JH & Warnich L, Euphytica, 113 (2000) 135.CrossRefGoogle Scholar
  21. 21.
    Anderson JA, Churchill GA, Autrique JE, Tanksley SD & Sorrells ME, Genome, 36 (1993) 181.PubMedCrossRefGoogle Scholar
  22. 22.
    Roldan-Ruiz I, Calsyn E, Gilliland TJ, Coll R, van Eijk MJT & De Loose M, Mol Breed, 6 (2000) 593.CrossRefGoogle Scholar
  23. 23.
    Jaccard P, Bull Soc Vaud Nat, 44 (1908) 223.Google Scholar
  24. 24.
    Sneath PHA & Sokal RR, Numerical taxonomy, WH Truman and Co., San Francisco (1973).Google Scholar
  25. 25.
    Mohammadi SA & Prasanna BM, Crop Sci, 43 (2003) 1235.CrossRefGoogle Scholar
  26. 26.
    Qi X, Pittaway TS, Lindup S, Liu H, Waterman E, Padi FK, Hash CT, Zhu J, Gale MD & Devos KM, Theor Appl Genet, 109 (2004) 1485.PubMedCrossRefGoogle Scholar
  27. 27.
    Rai KN, Talukdar BS, Singh SD, Rao AS, Rao AM & Andrews DJ, Crop Sci, 34 (1994) 1430.CrossRefGoogle Scholar
  28. 28.
    Kumar KA, Rai KN, Andrews DJ, Talukdar BS, Singh SD, Rao AS, Babu PP & Reddy BS, Crop Sci, 36 (1995) 605.CrossRefGoogle Scholar

Copyright information

© Springer 2007

Authors and Affiliations

  • A. C. Chandra-Shekara
    • 1
  • B. M. Prasanna
    • 1
  • S. R. Bhat
    • 2
  • B. B. Singh
    • 1
  1. 1.Division of GeneticsIndian Agricultural Research InstituteNew DelhiIndia
  2. 2.National Research Center on Plant BiotechnologyIndian Agricultural Research InstituteNew DelhiIndia

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