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Efficient In vitro Plant Regeneration Protocol from Leaf Explant of Jatropha curcas L — A Promising Biofuel Plant

  • Shrawan Kumar
  • Suman KumariaEmail author
  • Pramod Tandon
Short Communication

Abstract

An efficient in vitro plant regeneration from leaf-disc culture of Jatropha curcas L has been established. Adventitious shoot buds along with callus were induced from leaves of 2-year-old J. curcas plants cultured on Murashige and Skoog’s (MS) medium supplemented with TDZ (2 μM) BAP (2 μM) and IBA (1 μM), wherein 63.3% leaf explants responded. The multiplication of shoots was achieved from the adventitious shoot buds after transferring them to shoot induction medium. The highest number of shoots (9.7/explant) was achieved after 6 weeks of culture on MS medium containing 3 μM of BAR The welldeveloped shoots were rooted on MS medium supplemented with IBA (1.5 μM) with the rooting frequency of 53.3%. Addition of phloroglucinol (200 μM) to the medium enhanced the frequency of rooting to 76.7%. Regenerated plantlets were successfully transferred to field after initial acclimatization.

Key words

Adventitious shoot buds Jatropha curcas growth regulators plant regeneration 

Abbreviations

BAP

6-benzylaminopurine

IBA

indole-3-butydcacid

MS

Murashige and Skoog

SE

Standard error

TDZ

thidiazuron

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Copyright information

© Springer 2010

Authors and Affiliations

  1. 1.Plant Biotechnology Laboratory, Department of BotanyNorth-Eastern Hill UniversityShillongIndia

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