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Comparative Assessment of ISSR and RAPD Marker Assays for Genetic Diversity Analysis in Jojoba [Simmondsia chinensis (Link) Schneider]

Abstract

A collection of male and female plants of ten Jojoba [Simmondsia chinensis (Link) Schneider] genotypes was analyzed with 50 RAPID and 55 ISSR markers to compare the efficiency and utility of these techniques for detecting genetic polymorphism. RAPID and ISSR analysis yielded 442 and 566 scorable amplified products, respectively, of which 60.7 and 69.3% were polymorphic. ISSRs revealed efficiency over RAPDs due to high EMR (effective multiplex ratio), DI (diversity index, mean PIC per primer) and MI (marker index) values. Jaccard similarity matrices among male plants, among female plants and between male and female plants of the ten jojoba genotypes varied between 0.705-0.784. Dendrograms generated by cluster analysis (UPGMA, NTSYS-pc) supported by bootstrap values using RAPID and ISSR datasets led to grouping of most of male and females genotypes in separate clusters. While pattern of clustering remained more or less same, the two dendrograms did differ with respect to the grouping of a few male and female genotypes. The value of the Mantel test shows poor correlation (r = 0.41) between ISSR and RAPID marker datasets.

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Abbreviations

RAPD:

random amplified polymorphic DNA

ISSR:

inter simple sequence repeat

PIC:

polymorphic information content

UPGMA:

unweighted paired group method using arithmetic averages

NTSYS:

Numerical Taxonomy and Multivariate Analysis System

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Correspondence to Meenakshi Bhardwaj.

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Bhardwaj, M., Uppal, S., Jain, S. et al. Comparative Assessment of ISSR and RAPD Marker Assays for Genetic Diversity Analysis in Jojoba [Simmondsia chinensis (Link) Schneider]. J. Plant Biochem. Biotechnol. 19, 255–258 (2010). https://doi.org/10.1007/BF03263351

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Key words

  • genetic diversity
  • ISSR
  • jojoba
  • molecular markers
  • RAPID