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Comparative Assessment of ISSR and RAPD Marker Assays for Genetic Diversity Analysis in Jojoba [Simmondsia chinensis (Link) Schneider]

  • Meenakshi BhardwajEmail author
  • Sanjogta Uppal
  • Sunita Jain
  • Pushpa Kharb
  • Ravinder Dhillon
  • Rajinder K. Jain
Short Communication

Abstract

A collection of male and female plants of ten Jojoba [Simmondsia chinensis (Link) Schneider] genotypes was analyzed with 50 RAPID and 55 ISSR markers to compare the efficiency and utility of these techniques for detecting genetic polymorphism. RAPID and ISSR analysis yielded 442 and 566 scorable amplified products, respectively, of which 60.7 and 69.3% were polymorphic. ISSRs revealed efficiency over RAPDs due to high EMR (effective multiplex ratio), DI (diversity index, mean PIC per primer) and MI (marker index) values. Jaccard similarity matrices among male plants, among female plants and between male and female plants of the ten jojoba genotypes varied between 0.705-0.784. Dendrograms generated by cluster analysis (UPGMA, NTSYS-pc) supported by bootstrap values using RAPID and ISSR datasets led to grouping of most of male and females genotypes in separate clusters. While pattern of clustering remained more or less same, the two dendrograms did differ with respect to the grouping of a few male and female genotypes. The value of the Mantel test shows poor correlation (r = 0.41) between ISSR and RAPID marker datasets.

Key words

genetic diversity ISSR jojoba molecular markers RAPID 

Abbreviations

RAPD

random amplified polymorphic DNA

ISSR

inter simple sequence repeat

PIC

polymorphic information content

UPGMA

unweighted paired group method using arithmetic averages

NTSYS

Numerical Taxonomy and Multivariate Analysis System

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Copyright information

© Springer 2010

Authors and Affiliations

  • Meenakshi Bhardwaj
    • 1
    Email author
  • Sanjogta Uppal
    • 1
  • Sunita Jain
    • 2
  • Pushpa Kharb
    • 3
  • Ravinder Dhillon
    • 4
  • Rajinder K. Jain
    • 3
  1. 1.Department of GeneticsCCS Haryana Agricultural UniversityHisarIndia
  2. 2.Bioinformatics SectionCCS Haryana Agricultural UniversityHisarIndia
  3. 3.Department of Biotechnology and Molecular BiologyCCS Haryana Agricultural UniversityHisarIndia
  4. 4.Department of ForestryCCS Haryana Agricultural UniversityHisarIndia

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