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Isolation and Characterization of Plastidial Localized, Seed Expressive Lysophosphatidyl Acyl Transferase (LPAAT) cDNA from Brassica juncea L

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Abstract

In order to characterize different forms of Lysophosphatidyl acyl transferase (LPAAT) gene and to study its cellular location, PCR amplification of cDNA isolated from B. juncea was carried out using LPAAT gene specific primers. The amplicon obtained was of 1009 by and showed homology with BAT2 clone of B. napus. A multiple sequence analysis of LPAAT derived from different sources revealed that there were ten strongly conserved domains among different isoforms of LPAAT products with two most prominent conserved domain PEGTRS and NH. Southern blot analysis showed that there were two different isoforms of LPAAT gene in B. juncea. Northern blot analysis showed that maximum expression of the transcript was in case of seeds and low level in buds. However, the transcript was not detectable in leaves.

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Abbreviations

LPAAT:

Lysophosphatidyl acyl transferase

TAG:

Triacylglycerol

BAT:

Brassica acyl transferase

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Authors and Affiliations

  1. Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, 110 012, India

    Ranjeet R. Kumar & I. M. Santha

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  1. Ranjeet R. Kumar
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  2. I. M. Santha
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Correspondence to I. M. Santha.

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Kumar, R.R., Santha, I.M. Isolation and Characterization of Plastidial Localized, Seed Expressive Lysophosphatidyl Acyl Transferase (LPAAT) cDNA from Brassica juncea L. J. Plant Biochem. Biotechnol. 18, 225–228 (2009). https://doi.org/10.1007/BF03263324

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  • DOI: https://doi.org/10.1007/BF03263324

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