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Housekeeping Gene Selection for Real Time-PCR Normalization in Female Hop (Humulus lupulus L) Tissues

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Abstract

The variability of transcript accumulation of six genes encoding Chloropyll-a/b (Chla/b) binding protein, Nicotinamide Adenine Dinucleotide Hydride (NADH) dehydrogenase, Histone H3 (H3), DEAD-box RNA helicase 1 (DRH1) and Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and of the 7SL component of the signal recognition particle (7SL-RNA), was estimated in leaves and female inflorescences of the two hop cultivars, White Golding and Admiral at different developmental stages by RT-PCR. The value of these genes as internal, normalization controls in gene transcript accumulation studies was assessed. The combination of the three housekeeping genes DRH1, GAPDH and 7SL-RNA as internal standards for hop, provided reliable results in the quantitative analysis of the transcript accumulation of Hua Enhancer 1 transcription factor (HEN1) homologue in hop tissues and is recommended for future studies of gene expression in female hop tissues.

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Abbreviations

Chla/b:

Chloropy1l-a/b

NADH:

Nicotinamide Adenine Dinucleotide Hydride

DRH1:

DEAD-box RNA Helicase 1

GAPDH:

Glyceraldehyde-3-Phosphate Dehydrogenase

H3:

Histone 3

7SL-RNA:

7SL component of the signal recognition particle

HEN1:

Hua Enhancer 1

Ct-value:

the point at which the measured fluorescence of PCR products crosses a threshold value

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Correspondence to Isabel Roldán-Ruiz.

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Maloukh, L., Matousek, J., Van Bockstaele, E. et al. Housekeeping Gene Selection for Real Time-PCR Normalization in Female Hop (Humulus lupulus L) Tissues. J. Plant Biochem. Biotechnol. 18, 53–58 (2009). https://doi.org/10.1007/BF03263295

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  • DOI: https://doi.org/10.1007/BF03263295

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