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Somatic Embryogenesis in Capparis decidua (Forsk) Edgew — A Multipurpose Agroforestry Plant

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Abstract

An efficient protocol for direct somatic embryogenesis in Capparis decidua has been developed. Mature zygotic embryos cultured in Murashige and Skoog (MS) liquid medium with 2,4-0 (0.1 mg l−1) and BA (0.5 mg l−1) produced somatic embryos directly without an intervening callus phase from the subepidermal cells. Treatment with ABA promoted maturation of somatic embryos and BA (1 mg l−1)promoted germination. One zygotic embryo produced approximately 230 somatic embryos within 14 to 15 weeks. Embryos germinated in eight weeks and acclimatized plants were transferred to pots.

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Abbreviations

ABA:

Abscisic acid

BA:

Benzyladenine

2,4-D:

2,4-dichlorophenoxyacetic acid

GA3:

Gibberellic acid

IBA:

Indole-3-butyric acid

MS:

Murashige and Skoog

TBA:

Tertiary butyl alcohol

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Authors and Affiliations

  1. Department of Botany, Universityof Rajasthan, Jaipur, 302 004, India

    Purnima Tyagi, Shruti Khanduja & S. L. Kothari

Authors
  1. Purnima Tyagi
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  2. Shruti Khanduja
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  3. S. L. Kothari
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Corresponding author

Correspondence to S. L. Kothari.

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Tyagi, P., Khanduja, S. & Kothari, S.L. Somatic Embryogenesis in Capparis decidua (Forsk) Edgew — A Multipurpose Agroforestry Plant. J. Plant Biochem. Biotechnol. 14, 197–200 (2005). https://doi.org/10.1007/BF03263246

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  • DOI: https://doi.org/10.1007/BF03263246

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